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thiamine
?
there is degradation of thiamine without a base substrate, possibly the ring-opened form of thiamine functions as the nucleophile
-
-
?
thiamine + 4-nitrothiophenolate
4-methyl-5-(2-hydroxyethyl)thiazole + 2-methyl-5[[(4-nitrophenyl)sulfanyl]methyl]pyrimidin-4-amine
no activity with thiaminase II
-
-
?
thiamine + veratrylamine
bis-(4-amino-2-methyl)pyrimidinyl-3,4-dimethoxybenzylamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
the reaction can occur without base substrate
-
?
thiamine + 4-nitrothiophenolate
?
-
-
-
?
thiamine + aniline
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
thiamine + pyridine
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
thiamine + quinoline
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium
-
-
-
-
?
additional information
?
-
-
primary substrates inactivate the enzyme at 0.006 mM and secondary substrates or acceptor bases reactivate the enzyme at 10 mM, each substrate at high concentrations eliminates the inactivation of the other substrate
-
-
?
thiamine + aniline
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
-
-
?
thiamine + aniline
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
-
-
?
thiamine + aniline
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
-
-
?
thiamine + aniline
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
-
-
?
thiamine + aniline
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
one molecule of thiamine is cleaved releasing one molecule of methylhydroxyethylthiazole for one molecule of enzyme
-
?
thiamine + pyridine
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
-
-
?
thiamine + pyridine
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
-
-
?
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Fujita, A.
Thiaminase
Adv. Enzymol. Relat. Subj. Biochem.
15
389-421
1954
Acanthogobius flavimanus, Anadara inflata, Aneurinibacillus aneurinilyticus, Aneurinibacillus aneurinilyticus BKA, Anguilla japonica, Athyrium nipponicum, Auxis hira, Carassius auratus, Celosia crista, Charybdis 6-dentata, Circe scripta, Clava kochi, Corbicula leana, Cristaria plicata, Cyprinus carpio, Dicranopteris linearis, Dicranopteris sp., Dryopteris erythrosora, Dryopteris lacera, Eleotris oxycephala, Equisetum arvense, Eviota abax, Gnathopogon mayedae, Haliotis gigantea, Haliotis japonica, Hypomesus olidus, Ischikauia steenackeri, Leptochilus ellipticus, Leucopsarion petersii, Lycopodium clavatum, Mactra quadrangularis, Mactra sulcataria, Meretrix meretrix, Misgurnus anguillicaudatus, Mugil cephalus, no activity in Astroconger myriaster, no activity in Cambaroides japonicus, no activity in Camposcia retusa, no activity in Metapenaeopsis acclivis, no activity in Plecoglossus altivelis, no activity in Squilla oratoria, Odontobutis obscura, Osmunda japonica, Ostrea laperousei, Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus BMM, Panulirus japonicus, Paphia philippinarum, Polystichum aculeatum, Polystichum falcatum, Polystichum fortunei, Polystichum tsus-simense, Portunus trituberculatus, Pteridium aquilinum, Rhinogobius similis, Silurus asotus, Solen gouldi, Struthiopteris nipponica, Thiara libertina, Turbo cornutus, Umbonium costatum, Viviparus malleatus
brenda
Wittliff, J.L.; Airth, R.L.
The extracellular thiaminase I of Bacillus thiaminolyticus. I. Purification and physicochemical properties
Biochemistry
7
736-744
1968
Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus M
brenda
Suzuki, K.; Ooba, J.I.
Reversible inactivation of cellular thiaminase I in Bacillus thiaminolyticus by thiamine and heteropyrithiamine
J. Biochem.
72
1053-1055
1972
Paenibacillus thiaminolyticus
brenda
Suzuki, K.; Ooba, J.I.
Reversible inactivation of extracellular thiaminase I in Bacillus thiaminolyticus. I. Inactivation by the primary substrate and reactivation by the secondary substrate
Biochim. Biophys. Acta
293
111-117
1973
Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus YUSM I00I
brenda
Agee, C.C.; Airth, R.L.
Reversible inactivation of thiaminase I of Bacillus thiaminolyticus by its primary substrate, thiamine
J. Bacteriol.
115
957-965
1973
Paenibacillus thiaminolyticus
brenda
Hutter, J.A.; Slama, J.T.
Inhibition of thiaminase I from Bacillus thiaminolyticus. Evidence supporting a covalent 1,6-dihydropyrimidinyl-enzyme intermediate
Biochemistry
26
1969-1973
1987
Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus BMM
brenda
Costello, C.A.; Kelleher, N.L.; Abe, M.; McLafferty, F.W.; Begley, T.P.
Mechanistic studies on thiaminase I. Overexpression and identification of the active site nucleophile
J. Biol. Chem.
271
3445-3452
1996
Paenibacillus thiaminolyticus (P45741), Paenibacillus thiaminolyticus
brenda
Kelleher, N.L.; Nicewonger, R.B.; Begley, A.P.; McLafferty, F.W.
Identification of modification sites in large biomolecules by stable isotope labeling and tandem high resolution mass spectrometry. The active site nucleophile of thiaminase I
J. Biol. Chem.
272
32215-32220
1997
Paenibacillus thiaminolyticus (P45741)
brenda
Ruml, T.; Silhankova, L.; Brunerova, M.
Purification of thiaminase I for analytical purposes
Potravinarske Vedy
13
181-187
1995
Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus DBM 1068
-
brenda
Campobasso, N.; Begun, J.; Costello, C.A.; Begley, T.P.; Ealick, S.E.
Crystallization and preliminary x-ray analysis of thiaminase I from Bacillus thiaminolyticus: space group change upon freezing of crystals
Acta Crystallogr. Sect. D
54
448-450
1998
Paenibacillus thiaminolyticus (P45741), Paenibacillus thiaminolyticus
brenda
Wu, M.; Papish, E.T.; Begley, T.P.
Mechanistic studies on thiaminase I. Identification of the product of thiamin degradation in the absence of the nucleophilic cosubstrate
Bioorg. Chem.
28
45-48
2000
Paenibacillus thiaminolyticus (P45741)
-
brenda
Hanes, J.W.; Kraft, C.E.; Begley, T.P.
An assay for thiaminase I in complex biological samples
Anal. Biochem.
368
33-38
2007
Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus (P45741)
brenda
Soriano, E.V.; Rajashankar, K.R.; Hanes, J.W.; Bale, S.; Begley, T.P.; Ealick, S.E.
Structural similarities between thiamin-binding protein and thiaminase-I suggest a common ancestor
Biochemistry
47
1346-1357
2008
Paenibacillus thiaminolyticus (P45741)
brenda
Liu, S.; Monks, N.R.; Hanes, J.W.; Begley, T.P.; Yu, H.; Moscow, J.A.
Sensitivity of breast cancer cell lines to recombinant thiaminase I
Cancer Chemother. Pharmacol.
66
171-179
2010
Paenibacillus thiaminolyticus
brenda
Liu, S.; Bae, Y.; Leggas, M.; Daily, A.; Bhatnagar, S.; Miriyala, S.; St Clair, D.K.; Moscow, J.A.
Pharmacologic properties of polyethylene glycol-modified Bacillus thiaminolyticus thiaminase I enzyme
J. Pharmacol. Exp. Ther.
341
775-783
2012
Paenibacillus thiaminolyticus
brenda
Kraft, C.; Gordon, E.; Angert, E.
A rapid method for assaying thiaminase I activity in diverse biological samples
PLoS ONE
9
e92688
2014
Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus 8103
brenda