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Information on EC 2.5.1.19 - 3-phosphoshikimate 1-carboxyvinyltransferase and Organism(s) Mycobacterium tuberculosis and UniProt Accession P9WPY5

for references in articles please use BRENDA:EC2.5.1.19
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This record set is specific for:
Mycobacterium tuberculosis
UNIPROT: P9WPY5 not found.
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Word Map
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  • 2.5.1.19
  • excitatory
  • postsynaptic
  • depolarization
  • ipsps
  • afferent
  • monosynaptic
  • hippocampal
  • pyramidal
  • presynaptic
  • synapses
  • latency
  • fire
  • nmda
  • motoneuron
  • interneurons
  • glyphosate
  • firing
  • hyperpolarization
  • electrophysiological
  • antidromic
  • summation
  • subthreshold
  • bicuculline
  • gabaa
  • disynaptic
  • non-nmda
  • tetanic
  • 6-cyano-7-nitroquinoxaline-2,3-dione
  • volley
  • schaffer
  • quantal
  • unitary
  • paired-pulse
  • orthodromic
  • glyphosate-resistant
  • short-latency
  • thalamocortical
  • biotechnology
  • electrotonic
  • reticulospinal
  • radiatum
  • homonymous
  • drug development
  • agriculture
  • synthesis
  • interstimulus
  • funiculus
  • suprathreshold
  • preganglionic
  • low-threshold
  • impale
  • decerebrate
  • biocytin
  • afterhyperpolarization
The taxonomic range for the selected organisms is: Mycobacterium tuberculosis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
epsps, 5-enolpyruvylshikimate-3-phosphate synthase, epsp synthase, cp4 epsps, mepsps, 5-enolpyruvylshikimate 3-phosphate synthase, 5-enol-pyruvylshikimate-3-phosphate synthase, caepsps, 5-enol-pyruvyl-shikimate-3-phosphate synthase, 3-phosphoshikimate 1-carboxyvinyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mycobacterium tuberculosis 5-enolpyruvylshikimate-3-phosphate synthase
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3-enolpyruvylshikimate 5-phosphate synthase
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3-enolpyruvylshikimic acid-5-phosphate synthetase
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5'-enolpyruvylshikimate-3-phosphate synthase
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5-enolpyruvyl-3-phosphoshikimate synthase
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5-enolpyruvylshikimate-3-phosphate synthase
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5-enolpyruvylshikimate-3-phosphate synthetase
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5-enolpyruvylshikimate-3-phosphoric acid synthase
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5-enolpyruvylshikimic acid-3-phosphate synthase
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enolpyruvylshikimate phosphate synthase
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EPSP synthase
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synthase, 5-enolpyruvoylshikimate 3-phosphate
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
enolpyruvate group transfer
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SYSTEMATIC NAME
IUBMB Comments
phosphoenolpyruvate:3-phosphoshikimate 5-O-(1-carboxyvinyl)-transferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9068-73-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphoenolpyruvate + 3-phosphoshikimate
phosphate + 5-enolpyruvylshikimate 3-phosphate
show the reaction diagram
phosphoenolpyruvate + 3-phosphoshikimate
phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphoenolpyruvate + 3-phosphoshikimate
phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
show the reaction diagram
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the 5-enolpyruvylshikimate-3-phosphate synthase catalyses the sixth step of the shikimate pathway that is responsible for synthesizing aromatic compounds
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?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glyphosate
Kd: 350 micromolar (pH 6.9, 25°C), binding induces conformational change that leads to increase in alpha helix (40% to 57%) and decrease in beta-sheet (30% to 23%) content (revealed by circular dichroism spectroscopy) and prevents access of solvent to core molecule (revealed by hydrogen/deuterium exchange, peptic digestion and LC-ESI-MS)
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18.13
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recombinant enzyme
5.68
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activity of recombinant enzyme in crude extract
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46430
46425
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x * 46425, deduced from nucleotide sequence
46458
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x * 46458, electrospray ionization mass spectrometry
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
restrained-based, molecular modelling; open conformation model of apoenzyme generated based on coordinates from PDB: 2BJB (MtEPSPS), closed conformation model of enzyme/3-phosphoshikimate/glyphosate complex generated based on PDB: 1G6S
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, ammonium sulfate precipitate, 4 months, no loss of activity
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme, Q-Sepharose, one step purification
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli
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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
pellet from 80% (w/v) ammonium sulfate solution, resolubilization and dialysis against 5 mM sodium phosphate, pH 6.9
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Oliveira, J.S.; Pinto, C.A.; Basso, L.A.; Santos, D.S.
Cloning and Overexpression in Soluble Form of Functional Shikimate Kinase and 5-Enolpyruvylshikimate 3-Phosphate Synthase Enzymes from Mycobacterium tuberculosis
Protein Expr. Purif.
22
430-435
2001
Mycobacterium tuberculosis
Manually annotated by BRENDA team
Oliveira, J.S.; Mendes, M.A.; Palma, M.S.; Basso, L.A.; Santos, D.S.
One-step purification of 5-enolpyruvylshikimate-3-phosphate synthase enzyme from Mycobacterium tuberculosis
Protein Expr. Purif.
28
287-292
2003
Mycobacterium tuberculosis
Manually annotated by BRENDA team
Borges, J.C.; Pereira, J.H.; Vasconcelos, I.B.; dos Santos, G.C.; Olivieri, J.R.; Ramos, C.H.; Palma, M.S.; Basso, L.A.; Santos, D.S.; de Azevedo, W.F.
Phosphate closes the solution structure of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) from Mycobacterium tuberculosis
Arch. Biochem. Biophys.
452
156-164
2006
Mycobacterium tuberculosis
Manually annotated by BRENDA team
Marques, M.R.; Vaso, A.; Neto, J.R.; Fossey, M.A.; Oliveira, J.S.; Basso, L.A.; dos Santos, D.S.; de Azevedo Junior, W.F.; Palma, M.S.
Dynamics of glyphosate-induced conformational changes of Mycobacterium tuberculosis 5-enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) determined by hydrogen-deuterium exchange and electrospray mass spectrometry
Biochemistry
47
7509-7522
2008
Mycobacterium tuberculosis (P9WPY5), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WPY5)
Manually annotated by BRENDA team