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Information on EC 2.5.1.142 - nerylneryl diphosphate synthase and Organism(s) Solanum lycopersicum and UniProt Accession K7WQ45

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IUBMB Comments
Isolated from the plant Solanum lycopersicum (tomato).
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This record set is specific for:
Solanum lycopersicum
UNIPROT: K7WQ45
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The taxonomic range for the selected organisms is: Solanum lycopersicum
The enzyme appears in selected viruses and cellular organisms
Synonyms
cis-prenyl transferase, CPT1, CPT2, NNPP synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NNPP synthase
-
-
SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:isopentenyl-diphosphate cistransferase (adding 3 isopentenyl units)
Isolated from the plant Solanum lycopersicum (tomato).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + 3 isopentenyl diphosphate
3 diphosphate + nerylneryl diphosphate
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate
3 diphosphate + nerylneryl diphosphate
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + Z,Z-farnesyl diphosphate
?
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + 3 isopentenyl diphosphate
3 diphosphate + nerylneryl diphosphate
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate
3 diphosphate + nerylneryl diphosphate
show the reaction diagram
-
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0038
isopentenyl diphosphate
-
at pH 7.5 and 30°C
0.0229
Z,Z-farnesyl diphosphate
-
at pH 7.5 and 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0026
isopentenyl diphosphate
-
at pH 7.5 and 30°C
0.0026
Z,Z-farnesyl diphosphate
-
at pH 7.5 and 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.68
isopentenyl diphosphate
-
at pH 7.5 and 30°C
0.11
Z,Z-farnesyl diphosphate
-
at pH 7.5 and 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CPT2_SOLLC
314
0
36020
Swiss-Prot
Chloroplast (Reliability: 3)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HIS-Select HF nickel affinity Gel chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Akhtar, T.A.; Matsuba, Y.; Schauvinhold, I.; Yu, G.; Lees, H.A.; Klein, S.E.; Pichersky, E.
The tomato cis-prenyltransferase gene family
Plant J.
73
640-652
2013
Solanum lycopersicum (K7WQ45)
Manually annotated by BRENDA team
Matsuba, Y.; Zi, J.; Jones, A.D.; Peters, R.J.; Pichersky, E.
Biosynthesis of the diterpenoid lycosantalonol via nerylneryl diphosphate in Solanum lycopersicum
PLoS ONE
10
e0119302
2015
Solanum lycopersicum
Manually annotated by BRENDA team
Pelot, K.A.; Hagelthorn, D.M.; Hong, Y.J.; Tantillo, D.J.; Zerbe, P.
Diterpene synthase-catalyzed biosynthesis of distinct clerodane stereoisomers
Chembiochem
20
111-117
2020
Solanum lycopersicum
Manually annotated by BRENDA team