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Information on EC 2.5.1.137 - 2-acyl-4-prenylphloroglucinol 6-prenyltransferase and Organism(s) Humulus lupulus and UniProt Accession A0A0B4ZTQ2

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EC Tree
IUBMB Comments
The enzyme, characterized from hop (Humulus lupulus), catalyses two successive prenylations of a 2-acyl-4-prenylphloroglucinol during the synthesis of bitter acids. Forms a complex with EC 2.5.1.136, 2-acylphloroglucinol 4-prenyltransferase, which catalyses the initial prenylation of the substrates. Requires Mg2+.
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This record set is specific for:
Humulus lupulus
UNIPROT: A0A0B4ZTQ2
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The taxonomic range for the selected organisms is: Humulus lupulus
The expected taxonomic range for this enzyme is: Humulus lupulus
Synonyms
aromatic prenyltransferase, PT2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aromatic prenyltransferase
-
PT2
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:2-acyl-4-prenylphloroglucinol 6-dimethylallyltransferase
The enzyme, characterized from hop (Humulus lupulus), catalyses two successive prenylations of a 2-acyl-4-prenylphloroglucinol during the synthesis of bitter acids. Forms a complex with EC 2.5.1.136, 2-acylphloroglucinol 4-prenyltransferase, which catalyses the initial prenylation of the substrates. Requires Mg2+.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
diphosphate + ?
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
diphosphate + ?
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + a 2-acyl-4,6-bisprenylphloroglucinol
diphosphate + a 2-acyl-4,6,6-trisprenylcyclohexa-2,4-dien-1-one
show the reaction diagram
-
-
-
?
dimethylallyl diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
diphosphate + deoxycohumulone
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
diphosphate + deoxyhumulone
show the reaction diagram
-
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
prenyltransferase PT2 catalyzes the last two prenylation steps in the beta-bitter acid pathway. Coexpression of codon-optimized prenyltransferases PT1L and PT2 in yeast leads to the production of bitter acids. PT1L/PT2 forms and functions as a complex in membranes. PT1L catalyzes the first prenylation step, and PT2 catalyzes the two subsequent prenylation steps
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PT2_HUMLU
408
9
46172
Swiss-Prot
other Location (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
mutation of the Asp in Asp-rich motifs in PT1L to Ala and coexpression with wild-type prenyltransferase, the mutant strain does not produce any prenylated products. A truncated variant lacking 83 amino acid from the start codon, displays much higher activity than wild-type
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
HPLC assay for the prenylation reactions, measuring enzyme activities measured in protein extracts from hop flowerbudsflowers and young cones
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zuurbier, K.; Fung, S.; Scheffer, J.; Verpoorte, R.
In-vitro prenylation of aromatic intermediates in the biosynthesis of bitter acids in Humulus lupulus
Phytochemistry
49
2315-2322
1998
Humulus lupulus
-
Manually annotated by BRENDA team
Li, H.; Ban, Z.; Qin, H.; Ma, L.; King, A.J.; Wang, G.
A heteromeric membrane-bound prenyltransferase complex from hop catalyzes three sequential aromatic prenylations in the bitter acid pathway
Plant Physiol.
167
650-659
2015
Humulus lupulus (A0A0B4ZTQ2)
Manually annotated by BRENDA team