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Information on EC 2.5.1.112 - adenylate dimethylallyltransferase (ADP/ATP-dependent) and Organism(s) Arabidopsis thaliana and UniProt Accession Q9LJL4

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IUBMB Comments
Involved in the biosynthesis of cytokinins in plants. The IPT4 isoform from the plant Arabidopsis thaliana is specific for ADP and ATP . Other isoforms, such as IPT1 from Arabidopsis thaliana [1,2] and the enzyme from the common hop, Humulus lupulus , also have a lower activity with AMP (cf. EC 2.5.1.27, adenylate dimethylallyltransferase).
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Arabidopsis thaliana
UNIPROT: Q9LJL4
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
atp/adp ipt, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
adenylate isopentenyltransferase
-
ATP/ADP isopentenyltransferase
-
2-isopentenyl-diphosphate:ADP/ATP DELTA2-isopentenyltransferase
-
-
-
-
adenylate isopentenyltransferase
ATP/ADP isopentenyltransferase
dimethylallyl diphosphate:ATP/ADP isopentenyltransferase
SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:ADP/ATP dimethylallyltransferase
Involved in the biosynthesis of cytokinins in plants. The IPT4 isoform from the plant Arabidopsis thaliana is specific for ADP and ATP [1]. Other isoforms, such as IPT1 from Arabidopsis thaliana [1,2] and the enzyme from the common hop, Humulus lupulus [3], also have a lower activity with AMP (cf. EC 2.5.1.27, adenylate dimethylallyltransferase).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
show the reaction diagram
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
show the reaction diagram
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP
trans-zeatin riboside 5'-diphosphate + diphosphate
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
show the reaction diagram
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
show the reaction diagram
additional information
?
-
-
no activity with AMP and tRNA
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
show the reaction diagram
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
show the reaction diagram
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP
trans-zeatin riboside 5'-diphosphate + diphosphate
show the reaction diagram
-
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
show the reaction diagram
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.018
ATP
-
in 25 mM Tris-HCl, pH 7.5, 10 mM MgCl2+, 5 mM 2-mercaptoethanol at 24°C
0.0065
dimethylallyl diphosphate
-
in 25 mM Tris-HCl, pH 7.5, 10 mM MgCl2+, 5 mM 2-mercaptoethanol at 24°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
predominant expression of isoform IPT8
Manually annotated by BRENDA team
immature seed, predominant expression of isoform IPT8
Manually annotated by BRENDA team
predominant expression of isoform IPT4
Manually annotated by BRENDA team
expression of isoform IPT3
Manually annotated by BRENDA team
predominant expression of isoform IPT1
Manually annotated by BRENDA team
predominant expression of isoform IPT7
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
the ipt1357 quadruple mutant possesses severely decreased levels of isopentenyladenine and trans-zeatin , and their corresponding ribosides, ribotides, and glucosides, and is retarded in its growth. In contrast, these mutants possess increased levels of cis-zeatin-type cytokinins
metabolism
the enzyme isoforms are responsible for the bulk of isopentenyladenine- and trans-zeatin-type cytokinin synthesis
malfunction
the ipt1357 quadruple mutant possesses severely decreased levels of isopentenyladenine and trans-zeatin , and their corresponding ribosides, ribotides, and glucosides, and is retarded in its growth. In contrast, these mutants possess increased levels of cis-zeatin-type cytokinins
metabolism
the enzyme isoforms are responsible for the bulk of isopentenyladenine- and trans-zeatin-type cytokinin synthesis
physiological function
interference of auxin distribution disrupts the cytokinin response and ATP/ADP isopentenyltransferase IPT5 expression, affecting stem cell initiation and meristem formation. Auxin response factor ARF3 mediates the auxin response during de novo organ regeneration. Mutations in ARF3 cause ectopic cytokinin biosynthesis via the misexpression of IPT5, and this disrupts organ regeneration. ARF3 directly binds to the promoter of IPT5 and negatively regulates IPT5 expression
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
IPT8_ARATH
330
0
37322
Swiss-Prot
Chloroplast (Reliability: 2)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli AD494(DE3)pLysS cells
-
expressed in Escherichia coli M15 cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
auxin response factor ARF3 directly binds to the promoter of IPT5 and negatively regulates IPT5 expression
isoform IPT1 is down-regulated by the cytokinin 4-(indol-3-yl)butyric acid within 4 h
isoform IPT3 is down-regulated by the cytokinin 4-(indol-3-yl)butyric acid within 4 h
isoform IPT3 is rapidly induced by 1 h treatment with nitrate
isoform IPT5 is down-regulated by the cytokinin 4-(indol-3-yl)butyric acid within 4 h
isoform IPT5 is up-regulated by 4,4'-isopropylidenediphenol (bisphenol A) treatment for 4 h
isoform IPT7 is down-regulated by the cytokinin 4-(indol-3-yl)butyric acid within 4 h
isoform IPT7 is up-regulated by 4,4'-isopropylidenediphenol (bisphenol A) treatment for 4 h
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Takei, K.; Dekishima, Y.; Eguchi, T.; Yamaya, T.; Sakakibara, H.
A new method for enzymatic preparation of isopentenyladenine-type and trans-zeatin-type cytokinins with radioisotope-labeling
J. Plant Res.
116
259-263
2003
Arabidopsis thaliana
Manually annotated by BRENDA team
Kakimoto, T.
Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate:ATP/ADP isopentenyltransferases
Plant Cell Physiol.
42
677-685
2001
Arabidopsis thaliana
Manually annotated by BRENDA team
Miyawaki, K.; Matsumoto-Kitano, M.; Kakimoto, T.
Expression of cytokinin biosynthetic isopentenyltransferase genes in Arabidopsis: tissue specificity and regulation by auxin, cytokinin, and nitrate
Plant J.
37
128-138
2004
Arabidopsis thaliana (Q93WC9), Arabidopsis thaliana (Q94ID1), Arabidopsis thaliana (Q94ID2), Arabidopsis thaliana (Q94ID3), Arabidopsis thaliana (Q9C6L1), Arabidopsis thaliana (Q9LJL4), Arabidopsis thaliana (Q9SB60)
Manually annotated by BRENDA team
Miyawaki, K.; Tarkowski, P.; Matsumoto-Kitano, M.; Kato, T.; Sato, S.; Tarkowska, D.; Tabata, S.; Sandberg, G.; Kakimoto, T.
Roles of Arabidopsis ATP/ADP isopentenyltransferases and tRNA isopentenyltransferases in cytokinin biosynthesis
Proc. Natl. Acad. Sci. USA
103
16598-16603
2006
Arabidopsis thaliana (Q93WC9), Arabidopsis thaliana (Q94ID1), Arabidopsis thaliana (Q94ID2), Arabidopsis thaliana (Q94ID3), Arabidopsis thaliana (Q9C6L1), Arabidopsis thaliana (Q9LJL4), Arabidopsis thaliana (Q9SB60), Arabidopsis thaliana
Manually annotated by BRENDA team
Cheng, Z.; Wang, L.; Sun, W.; Zhang, Y.; Zhou, C.; Su, Y.; Li, W.; Sun, T.; Zhao, X.; Li, X.; Cheng, Y.; Zhao, Y.; Xie, Q.; Zhang, X.
Pattern of auxin and cytokinin responses for shoot meristem induction results from the regulation of cytokinin biosynthesis by AUXIN RESPONSE FACTOR3
Plant Physiol.
161
240-251
2013
Arabidopsis thaliana (Q94ID2)
Manually annotated by BRENDA team