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Information on EC 2.5.1.100 - fumigaclavine A dimethylallyltransferase
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The expected taxonomic range for this enzyme is: Aspergillus fumigatus
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EC NUMBER 
COMMENTARY 
2.5.1.100
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RECOMMENDED NAME
GeneOntology No.
fumigaclavine A dimethylallyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
fumigaclavine A + dimethylallyl diphosphate = fumigaclavine C + diphosphate
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:fumigaclavine A dimethylallyltransferase
Fumigaclavine C is an ergot alkaloid produced by some fungi of the Trichocomaceae family. Activity does not require any metal ions.
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene fgaPT1, in the gene cluster of fumigaclabvine C biosynthesis
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
metabolism
evolution
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FgaPT1 shows very low sequence similarity to the recently identified prenyltransferase LtxC from cyanobacteria, which also catalyzes the reverse prenylation of an indole nucleus
evolution
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the enzyme belongs to the group of aromatic prenyltransferases
evolution
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FgaPT1 shows very low sequence similarity to the recently identified prenyltransferase LtxC from cyanobacteria, which also catalyzes the reverse prenylation of an indole nucleus
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metabolism
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FgaPT1 catalyzes the last step of fumigaclavine C biosynthesis, i.e. the prenylation of fumigaclavine A at C-2 position of the indole nucleus, which is included in the biosynthesis of ergot alkaloids
metabolism
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FgaPT1 catalyzes the last step of fumigaclavine C biosynthesis, i.e. the prenylation of fumigaclavine A at C-2 position of the indole nucleus, which is included in the biosynthesis of ergot alkaloids
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
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?
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
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the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus
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?
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
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the enzyme is specific for dimethylallyl diphosphate as prenyl donor, but shows a broad substrate spectrum for the aromatic substrate, overview
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?
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
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?
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
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the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus
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?
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
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?
additional information
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FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A
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additional information
?
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FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
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?
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
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?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
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no significant effects by Mg2+, Ca2+, and EDTA at 5 mM
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His6-tagged FgaPT1 from Escherichia coli strain BL21(DE3)pLysS to near homogeneity
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene fgaaPT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli or Saccharomyces cerevisiae
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gene fgaPT1, DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis
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gene fgaPT1, DNA and amino acid sequence determination and analysis, overexpression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
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LINKS TO OTHER DATABASES (specific for EC-Number 2.5.1.100)
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