We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 2.5.1.100 - fumigaclavine A dimethylallyltransferase for references in articles please use BRENDA:EC2.5.1.100
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Fumigaclavine C is an ergot alkaloid produced by some fungi of the Trichocomaceae family. Activity does not require any metal ions.
The expected taxonomic range for this enzyme is: Aspergillus fumigatus
Synonyms
FgaPT1 , fumigaclavine C synthase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
FgaPT1
-
-
-
-
fumigaclavine C synthase
-
-
fumigaclavine C synthase
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
fumigaclavine A + dimethylallyl diphosphate = fumigaclavine C + diphosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dimethylallyl-diphosphate:fumigaclavine A dimethylallyltransferase
Fumigaclavine C is an ergot alkaloid produced by some fungi of the Trichocomaceae family. Activity does not require any metal ions.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
additional information
?
-
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
-
-
-
-
?
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
-
the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus
-
-
?
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
-
the enzyme is specific for dimethylallyl diphosphate as prenyl donor, but shows a broad substrate spectrum for the aromatic substrate, overview
-
-
?
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
-
-
-
-
?
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
-
the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus
-
-
?
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
-
-
-
-
?
additional information
?
-
-
FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A
-
-
-
additional information
?
-
-
FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
-
-
-
-
?
fumigaclavine A + dimethylallyl diphosphate
fumigaclavine C + diphosphate
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
no significant effects by Mg2+, Ca2+, and EDTA at 5 mM
additional information
-
no requirement for divalent metal ions
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mn2+
-
90% inhibition at 5 mM
Zn2+
-
90% inhibition at 5 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.013
dimethylallyl diphosphate
-
pH 7.5, 30°C
0.006
fumigaclavine A
-
pH 7.5, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.8
dimethylallyl diphosphate
-
pH 7.5, 30°C
0.8
fumigaclavine A
-
pH 7.5, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
gene fgaPT1
-
-
brenda
gene fgaPT1, in the gene cluster of fumigaclabvine C biosynthesis
-
-
brenda
gene fgaPT1
-
-
brenda
gene fgaPT1, in the gene cluster of fumigaclabvine C biosynthesis
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
evolution
-
FgaPT1 shows very low sequence similarity to the recently identified prenyltransferase LtxC from cyanobacteria, which also catalyzes the reverse prenylation of an indole nucleus
evolution
-
the enzyme belongs to the group of aromatic prenyltransferases
evolution
-
FgaPT1 shows very low sequence similarity to the recently identified prenyltransferase LtxC from cyanobacteria, which also catalyzes the reverse prenylation of an indole nucleus
-
metabolism
-
FgaPT1 catalyzes the last step of fumigaclavine C biosynthesis, i.e. the prenylation of fumigaclavine A at C-2 position of the indole nucleus, which is included in the biosynthesis of ergot alkaloids
metabolism
-
FgaPT1 catalyzes the last step of fumigaclavine C biosynthesis, i.e. the prenylation of fumigaclavine A at C-2 position of the indole nucleus, which is included in the biosynthesis of ergot alkaloids
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EASL_ASPFU
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)
436
0
50196
Swiss-Prot
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
110000
-
recombinant His6-tagged FgaPT1, gel filtration
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dimer
-
2 x 53000, recombinant His6-tagged FgaPT1, SDS-PAGE
dimer
-
2 x 53000, recombinant His6-tagged FgaPT1, SDS-PAGE
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
recombinant His6-tagged FgaPT1 from Escherichia coli strain BL21(DE3)pLysS to near homogeneity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
gene fgaaPT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli or Saccharomyces cerevisiae
-
gene fgaPT1, DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis
-
gene fgaPT1, DNA and amino acid sequence determination and analysis, overexpression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Unsoeld, I.A.; Li, S.M.
Reverse prenyltransferase in the biosynthesis of fumigaclavine C in Aspergillus fumigatus: gene expression, purification, and characterization of fumigaclavine C synthase FGAPT1
ChemBioChem
7
158-164
2006
Aspergillus fumigatus, Aspergillus fumigatus Af293
brenda
Steffan, N.; Grundmann, A.; Yin, W.B.; Kremer, A.; Li, S.M.
Indole prenyltransferases from fungi: a new enzyme group with high potential for the production of prenylated indole derivatives
Curr. Med. Chem.
16
218-231
2009
Aspergillus fumigatus
brenda
Li, S.M.; Unsoeld, I.A.
Post-genome research on the biosynthesis of ergot alkaloids
Planta Med.
72
1117-1120
2006
Aspergillus fumigatus, Aspergillus fumigatus Af293
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
Enzyme Nomenclature
2.5.1.100 fumigaclavine A dimethylallyltransferase
top
Information
