Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.4.99.18 - dolichyl-diphosphooligosaccharide-protein glycotransferase and Organism(s) Halobacterium salinarum and UniProt Accession B0R4T2

for references in articles please use BRENDA:EC2.4.99.18
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
Occurs in eukaryotes that form a glycoprotein by the transfer of a glucosyl-mannosyl-glucosamine polysaccharide to the side-chain of an L-asparagine residue in the sequence -Asn-Xaa-Ser- or -Asn-Xaa-Thr- (Xaa not Pro) in nascent polypeptide chains. The basic oligosaccharide is the tetradecasaccharide Glc3Man9GlcNAc2 (for diagram {polysacc/Dol14}). However, smaller oligosaccharides derived from it and oligosaccharides with additional monosaccharide units attached may be involved. See ref for a review of N-glycoproteins in eukaryotes. Man3GlcNAc2 seems to be common for all of the oligosaccharides involved with the terminal N-acetylglucosamine linked to the protein L-asparagine. Occurs on the cytosolic face of the endoplasmic reticulum. The dolichol involved normally has 14-21 isoprenoid units with two trans double-bonds at the omega end, and the rest of the double-bonds in cis form.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Halobacterium salinarum
UNIPROT: B0R4T2
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Halobacterium salinarum
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Synonyms
oligosaccharyltransferase, oligosaccharyl transferase, oligosaccharyltransferase complex, ost3p, wbp1p, ost6p, oligosaccharide transferase, swp1p, tbstt3a, tbstt3b, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AglB
archaeal homolog to bacterial PglB and eukaryotic Stt3
oligosaccharyltransferase
-
asparagine N-glycosyltransferase
-
-
-
-
dolichyldiphosphooligosaccharide-protein oligosaccharyltransferase
-
-
-
-
glycosyltransferase, dolichyldiphosphooligosaccharide-protein
-
-
-
-
glycosyltransferase, dolichylpyrophosphodiacetylchitobiose-protein
-
-
-
-
oligomannosyltransferase
-
-
-
-
oligosaccharide transferase
-
-
-
-
oligosaccharyltransferase, dolichyldiphosphoryloligosaccharide-protein
-
-
-
-
OST
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
dolichyl-diphosphooligosaccharide:protein-L-asparagine N-beta-D-oligopolysaccharidotransferase
Occurs in eukaryotes that form a glycoprotein by the transfer of a glucosyl-mannosyl-glucosamine polysaccharide to the side-chain of an L-asparagine residue in the sequence -Asn-Xaa-Ser- or -Asn-Xaa-Thr- (Xaa not Pro) in nascent polypeptide chains. The basic oligosaccharide is the tetradecasaccharide Glc3Man9GlcNAc2 (for diagram {polysacc/Dol14}). However, smaller oligosaccharides derived from it and oligosaccharides with additional monosaccharide units attached may be involved. See ref [2] for a review of N-glycoproteins in eukaryotes. Man3GlcNAc2 seems to be common for all of the oligosaccharides involved with the terminal N-acetylglucosamine linked to the protein L-asparagine. Occurs on the cytosolic face of the endoplasmic reticulum. The dolichol involved normally has 14-21 isoprenoid units with two trans double-bonds at the omega end, and the rest of the double-bonds in cis form.
CAS REGISTRY NUMBER
COMMENTARY hide
75302-32-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dolichyl diphosphooligosaccharide + [protein]-L-asparagine
dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dolichyl diphosphooligosaccharide + [protein]-L-asparagine
dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
show the reaction diagram
-
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
despite processing distinct lipid-linked glycans in their native hosts, AglB from Halobacterium salinarum can readily replace their counterpart from Haloferax volcanii when introduced into Haloferax volcanii cells deleted of aglB
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Magidovich, H.; Eichler, J.
Glycosyltransferases and oligosaccharyltransferases in Archaea: putative components of the N-glycosylation pathway in the third domain of life
FEMS Microbiol. Lett.
300
122-130
2009
Archaeoglobus fulgidus (O29867), Archaeoglobus fulgidus (O29918), Archaeoglobus fulgidus (O30195), Caldivirga maquilingensis (A8MBU0), Candidatus Korarchaeum cryptofilum (B1L5S3), Desulfurococcus amylolyticus (B8D352), Haloarcula marismortui (Q5V4T7), Halobacterium salinarum (B0R4T2), Halobacterium salinarum NRC-1 (Q9HQP2), Haloquadratum walsbyi (Q18GV3), Halorubrum lacusprofundi (B9LMS1), Hyperthermus butylicus (A2BM28), Ignicoccus hospitalis (A8A8E8), Metallosphaera sedula (A4YHQ7), Methanobrevibacter smithii (A5UL43), Methanocaldococcus jannaschii (Q58920), Methanocella arvoryzae MRE50 (Q0W803), Methanocella arvoryzae MRE50 (Q0W817), Methanocella arvoryzae MRE50 (Q0W818), Methanococcoides burtonii (Q12VP3), Methanococcus aeolicus (A6UWW3), Methanococcus maripaludis C5 (A4FWA0), Methanococcus maripaludis C6 (A9A9N9), Methanococcus maripaludis C7 (A6VH10), Methanococcus maripaludis S2 (Q6LXC8), Methanococcus vannielii (A6UQ69), Methanocorpusculum labreanum (A2SR78), Methanoculleus marisnigri (A3CRV9), Methanoculleus marisnigri (A3CXQ8), Methanoregula boonei (A7I4W0), Methanoregula boonei (A7I7L6), Methanosarcina acetivorans (Q8TJM6), Methanosarcina acetivorans (Q8TJM7), Methanosarcina acetivorans (Q8TJM8), Methanosarcina acetivorans (Q8TRK3), Methanosarcina barkeri (Q46FJ0), Methanosarcina barkeri (Q46FW5), Methanosarcina barkeri (Q46FW6), Methanosarcina mazei (Q8PUW8), Methanosarcina mazei (Q8PZ47), Methanosarcina mazei (Q8PZ48), Methanosphaera stadtmanae (Q2NHD2), Methanosphaerula palustris (B8GG73), Methanospirillum hungatei (Q2FSJ2), Methanospirillum hungatei (Q2FTM5), Methanospirillum hungatei (Q2FUP2), Methanothermobacter thermautotrophicus str. Delta H (O27660), Methanothrix thermoacetophila (A0B8C2), Methanothrix thermoacetophila (A0B996), Nanoarchaeum equitans (Q74MN3), Natronomonas pharaonis (Q3IPK0), Nitrosopumilus maritimus (A9A114), no activity in Aeropyrum pernix, no activity in Methanopyrus kandleri, Picrophilus torridus (Q6L0Y1), Pyrobaculum aerophilum (Q8ZTY7), Pyrobaculum arsenaticum (A4WLR5), Pyrobaculum calidifontis (A3MUV5), Pyrobaculum islandicum (A1RRM7), Pyrobaculum neutrophilum (B1YAG2), Pyrococcus abyssi (Q9UYP5), Pyrococcus abyssi (Q9UZF5), Pyrococcus abyssi (Q9V250), Pyrococcus furiosus (Q8U3P6), Pyrococcus furiosus (Q8U4D2), Pyrococcus horikoshii (O58981), Pyrococcus horikoshii (O74088), Saccharolobus solfataricus (Q97Z77), Staphylothermus marinus (A3DL26), Sulfolobus acidocaldarius (Q4J9B4), Sulfurisphaera tokodaii (Q973G2), Thermococcus kodakarensis (Q5JH70), Thermococcus kodakarensis (Q5JJ26), Thermococcus onnurineus (B6YVI6), Thermococcus onnurineus (B6YVU0), Thermofilum pendens (A1RXW3), Thermoplasma acidophilum (Q9HJ37), Thermoplasma volcanium (Q979C6)
Manually annotated by BRENDA team
Cohen-Rosenzweig, C.; Guan, Z.; Shaanan, B.; Eichler, J.
Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a variety of lipid-linked glycans
Appl. Environ. Microbiol.
80
486-496
2014
Haloarcula marismortui, Halobacterium salinarum, Haloferax mediterranei, Haloferax volcanii, Haloferax volcanii DSM 3757
Manually annotated by BRENDA team