Information on EC 2.4.99.16 - starch synthase (maltosyl-transferring) and Organism(s) Mycobacterium smegmatis and UniProt Accession Q9RP48

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This record set is specific for:
Mycobacterium smegmatis
UNIPROT: Q9RP48


The taxonomic range for the selected organisms is: Mycobacterium smegmatis

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.99.16
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RECOMMENDED NAME
GeneOntology No.
starch synthase (maltosyl-transferring)
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycogen biosynthesis III (from alpha-maltose 1-phosphate)
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Starch and sucrose metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
alpha-maltose 1-phosphate:(1->4)-alpha-D-glucan 4-alpha-D-maltosyltransferase
The enzyme from the bacterium Mycobacterium smegmatis is specific for maltose. It has no activity with alpha-D-glucose.
CAS REGISTRY NUMBER
COMMENTARY hide
56093-22-2
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ORGANISM
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LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
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LITERATURE
metabolism
the enzyme catalyzes the last step in the conversion of trehalose to glycogen transfering maltose from maltose 1-phosphate to glycogen. Trehalose synthase, maltokinase, and GMPMT represent an additional pathway of glycogen synthesis using trehalose as the source of glucose
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
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LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
phosphate + [(1->4)-alpha-D-glucosyl]n+2
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
phosphate + [(1->4)-alpha-D-glucosyl]n+2
show the reaction diagram
Q9RP48
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?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,4-dideoxy-1,4-imino-D-arabinitol
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ATP
strong inhibition
D-glucose 1-phosphate
slight inhibition
D-glucose 6-phosphate
slight inhibition
diphosphate
slight inhibition
maltosaccharides
compete with glycogen for the transferred maltose
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
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LITERATURE
IMAGE
additional information
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
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LITERATURE
0.76
purified enzyme, pH 7.0, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
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LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
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LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme GMPMT 89fold by ammonium sulfate fractionation, dialysis, anion exchange chromatography and gel filtration