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Information on EC 2.4.2.48 - tRNA-guanine15 transglycosylase and Organism(s) Methanosarcina acetivorans and UniProt Accession Q8THU2

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.48 tRNA-guanine15 transglycosylase
IUBMB Comments
Archaeal tRNAs contain the modified nucleoside archaeosine at position 15. This archaeal enzyme catalyses the exchange of guanine at position 15 of tRNA with the base preQ0, which is ultimately modified to form the nucleoside archaeosine (cf. EC 2.6.1.97) .
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This record set is specific for:
Methanosarcina acetivorans
UNIPROT: Q8THU2
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The taxonomic range for the selected organisms is: Methanosarcina acetivorans
The expected taxonomic range for this enzyme is: Archaea, Bacteria
Reaction Schemes
guanine15 in tRNA
+
=
7-cyano-7-carbaguanine15 in tRNA
+
Synonyms
archaeosine trna-guanine transglycosylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
archaeosine tRNAguanine transglycosylase
-
transfer ribonucleic acid guanine15 transglycosylase
-
-
-
-
tRNA guanine15 transglycosidase
-
-
-
-
tRNA-guanine transglycosylase
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
tRNA-guanine15:7-cyano-7-carbaguanine tRNA-D-ribosyltransferase
Archaeal tRNAs contain the modified nucleoside archaeosine at position 15. This archaeal enzyme catalyses the exchange of guanine at position 15 of tRNA with the base preQ0, which is ultimately modified to form the nucleoside archaeosine (cf. EC 2.6.1.97) [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
guanine15 in tRNA + 7-cyano-7-carbaguanine
7-cyano-7-carbaguanine15 in tRNA + guanine
show the reaction diagram
-
-
-
?
guanine15 in tRNA + 7-cyano-7-deazaguanine
7-cyano-7-deazaguanine15 in tRNA + guanine
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
guanine15 in tRNA + 7-cyano-7-carbaguanine
7-cyano-7-carbaguanine15 in tRNA + guanine
show the reaction diagram
-
-
-
?
guanine15 in tRNA + 7-cyano-7-deazaguanine
7-cyano-7-deazaguanine15 in tRNA + guanine
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
maximum activity at 10 mM Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0012 - 0.0021
guanine15 in tRNA
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0311 - 0.0667
guanine15 in tRNA
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
27 - 31
guanine15 in tRNA
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27
native enzyme, after 2900fold purification, at pH 7.5 and 37°C
31
recombinant enzyme, after 14fold purification, at pH 7.5 and 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
150000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterotetramer
2 * 54000 + 2 * 19000
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, phenyl Sepharose column chromatography, HiTrap Q column chromatography, HiTrap heparin column chromatography, Mono Q column chromatography, and Sephacryl S-200 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli ER2566 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nomura, Y.; Onda, Y.; Ohno, S.; Taniguchi, H.; Ando, K.; Oka, N.; Nishikawa, K.; Yokogawa, T.
Purification and comparison of native and recombinant tRNA-guanine transglycosylases from Methanosarcina acetivorans
Protein Expr. Purif.
88
13-19
2013
Methanosarcina acetivorans (Q8THU2), Methanosarcina acetivorans, Methanosarcina acetivorans DSM 2834 (Q8THU2)
Manually annotated by BRENDA team
Nomura, Y.; Ohno, S.; Nishikawa, K.; Yokogawa, T.
Correlation between the stability of tRNA tertiary structure and the catalytic efficiency of a tRNA-modifying enzyme, archaeal tRNA-guanine transglycosylase
Genes Cells
21
41-52
2016
Methanosarcina acetivorans
Manually annotated by BRENDA team