Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.4.2.48 - tRNA-guanine15 transglycosylase and Organism(s) Pyrococcus horikoshii and UniProt Accession O58843

for references in articles please use BRENDA:EC2.4.2.48
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.48 tRNA-guanine15 transglycosylase
IUBMB Comments
Archaeal tRNAs contain the modified nucleoside archaeosine at position 15. This archaeal enzyme catalyses the exchange of guanine at position 15 of tRNA with the base preQ0, which is ultimately modified to form the nucleoside archaeosine (cf. EC 2.6.1.97) .
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Pyrococcus horikoshii
UNIPROT: O58843
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Pyrococcus horikoshii
The expected taxonomic range for this enzyme is: Archaea, Bacteria
Reaction Schemes
guanine15 in tRNA
+
=
7-cyano-7-carbaguanine15 in tRNA
+
Synonyms
archaeosine trna-guanine transglycosylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
archaeosine tRNA-guanine transglycosylase
-
transfer ribonucleic acid guanine15 transglycosylase
-
-
-
-
tRNA guanine15 transglycosidase
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
tRNA-guanine15:7-cyano-7-carbaguanine tRNA-D-ribosyltransferase
Archaeal tRNAs contain the modified nucleoside archaeosine at position 15. This archaeal enzyme catalyses the exchange of guanine at position 15 of tRNA with the base preQ0, which is ultimately modified to form the nucleoside archaeosine (cf. EC 2.6.1.97) [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
guanine15 in tRNA(Leu) + 7-cyano-7-carbaguanine
7-cyano-7-carbaguanine15 in tRNA(Leu) + guanine
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
contains a zinc-binding site
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure of tRNAVal in complex with archaeosine tRNA-guanine transglycosylase at 3.3 A resolution. The enzyme binds a different tRNA conformation (lambda form), in which the canonical core is completely disrupted and the melted D arm is protruded. The PUA domain is found to be crucial for the precise location of the tRNA molecule on the enzyme
crystal structures of the enzyme at 2.2 A resolution and its complexes with guanine and 7-cyano-7-deazaguanine, at 2.3 and 2.5 A resolutions, respectively
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in E. coli BL21 (DE3) Rosetta 2 strain
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ishitani, R.; Nureki, O.; Nameki, N.; Okada, N.; Nishimura, S.; Yokoyama, S.
Alternative tertiary structure of tRNA for recognition by a posttranscriptional modification enzyme
Cell
113
383-394
2005
Pyrococcus horikoshii (O58843)
Manually annotated by BRENDA team
Ishitani, R.; Nureki, O.; Fukai, S.; Kijimoto, T.; Nameki, N.; Watanabe, M.; Kondo, H.; Sekine, M.; Okada, N.; Nishimura, S.; Yokoyama, S.
Crystal structure of archaeosine tRNA-guanine transglycosylase
J. Mol. Biol.
318
665-677
2002
Pyrococcus horikoshii (O58843), Pyrococcus horikoshii
Manually annotated by BRENDA team
Kawamura, T.; Hirata, A.; Ohno, S.; Nomura, Y.; Nagano, T.; Nameki, N.; Yokogawa, T.; Hori, H.
Multisite-specific archaeosine tRNA-guanine transglycosylase (ArcTGT) from Thermoplasma acidophilum, a thermo-acidophilic archaeon
Nucleic Acids Res.
44
1894-1908
2015
Pyrococcus horikoshii (O58843), Pyrococcus horikoshii, Pyrococcus horikoshii OT-3 (O58843), Thermoplasma acidophilum (Q9HI54), Thermoplasma acidophilum
Manually annotated by BRENDA team