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Information on EC 2.4.2.45 - decaprenyl-phosphate phosphoribosyltransferase and Organism(s) Mycobacterium tuberculosis and UniProt Accession P9WFR5

for references in articles please use BRENDA:EC2.4.2.45
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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.45 decaprenyl-phosphate phosphoribosyltransferase
IUBMB Comments
Requires Mg2+. Isolated from Mycobacterium tuberculosis. Has some activity with other polyprenyl phosphates.
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This record set is specific for:
Mycobacterium tuberculosis
UNIPROT: P9WFR5
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Word Map
The taxonomic range for the selected organisms is: Mycobacterium tuberculosis
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
rv3806c, 5-phosphoribosyltransferase, rv3806, dppr synthase, 5-phospho-alpha-d-ribose-1-diphosphate decaprenyl-phosphate 5-phosphoribosyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5-phospho-alpha-D-ribose-1-diphosphate: decaprenyl-phosphate
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5-phospho-alpha-D-ribose-1-diphosphate: decaprenyl-phosphate 5-phosphoribosyltransferase
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5-phospho-alpha-FS18 D-ribose-1-diphosphate:decaprenyl-phosphate 5-phosphoribosyltransferase
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5-phosphoribose-1-diphosphate: decaprenyl-phosphate 5-phosphoribosyltransferase
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5-phosphoribosyltransferase
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-phospho-alpha-D-ribose-1-diphosphate:decaprenyl-phosphate 5-phosphoribosyltransferase
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-
-
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5-phospho-alpha-D-ribose 1-pyrophosphate:decaprenyl phosphate 5-phosphoribosyltransferase
-
-
-
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DPPR synthase
-
-
-
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Rv3806
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate:diphosphate phospho-alpha-D-ribosyltransferase
Requires Mg2+. Isolated from Mycobacterium tuberculosis. Has some activity with other polyprenyl phosphates.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate
trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate
trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
x * 30000, SDS-PAGE
33000
x * 33000, estimated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A35E
the mutant is associated with high-level ethambutol resistance and increases decaprenylphosphoryl-beta-D-arabinose levels
D77E
15% activity compared to the wild type enzyme
E195A
17% activity compared to the wild type enzyme
F176L
the mutation is associated with ethambutol resistance
F59A
94% activity compared to the wild type enzyme
F62A
72% activity compared to the wild type enzyme
G165C
the mutant is associated with high-level ethambutol resistance and increases decaprenylphosphoryl-beta-D-arabinose levels
H84L
15% activity compared to the wild type enzyme
K174T
the mutation is associated with ethambutol resistance
N29A
18% activity compared to the wild type enzyme
N73Q
15% activity compared to the wild type enzyme
R201A
1% activity compared to the wild type enzyme
R22L
10% activity compared to the wild type enzyme
W175C
the mutation is associated with ethambutol resistance
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli ER2566 cells
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
the enzyme is a good target for the development of tuberculosis drugs
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Huang, H.; Scherman, M.S.; DHaeze, W.; Vereecke, D.; Holsters, M.; Crick, D.C.; McNeil, M.R.
Identification and active expression of the Mycobacterium tuberculosis gene encoding 5-phospho-alpha-D-ribose-1-diphosphate: decaprenyl-phosphate 5-phosphoribosyltransferase, the first enzyme committed to decaprenylphosphoryl-D-arabinose synthesis
J. Biol. Chem.
280
24539-24543
2005
Mycobacterium tuberculosis (P9WFR5), Mycobacterium tuberculosis
Manually annotated by BRENDA team
Huang, H.; Berg, S.; Spencer, J.S.; Vereecke, D.; DHaeze, W.; Holsters, M.; McNeil, M.R.
Identification of amino acids and domains required for catalytic activity of DPPR synthase, a cell wall biosynthetic enzyme of Mycobacterium tuberculosis
Microbiology
154
736-743
2008
Corynebacterium glutamicum, Mycobacterium tuberculosis (P9WFR5), Mycobacterium tuberculosis, Mycolicibacterium smegmatis, no activity in Azorhizobium caulinodans
Manually annotated by BRENDA team
Cai, L.; Zhao, X.; Jiang, T.; Qiu, J.; Owusu, L.; Ma, Y.; Wang, B.; Xin, Y.
Prokaryotic Expression, identification and bioinformatics analysis of the Mycobacterium tuberculosis Rv3807c gene encoding the putative enzyme committed to decaprenylphosphoryl-D-arabinose synthesis
Indian J. Microbiol.
54
46-51
2014
Mycobacterium tuberculosis (P9WFR5), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WFR5)
Manually annotated by BRENDA team
Safi, H.; Lingaraju, S.; Amin, A.; Kim, S.; Jones, M.; Holmes, M.; McNeil, M.; Peterson, S.N.; Chatterjee, D.; Fleischmann, R.; Alland, D.
Evolution of high-level ethambutol-resistant tuberculosis through interacting mutations in decaprenylphosphoryl-alpha-D-arabinose biosynthetic and utilization pathway genes
Nat. Genet.
45
1190-1197
2013
Mycobacterium tuberculosis (P9WFR5), Mycobacterium tuberculosis
Manually annotated by BRENDA team
He, L.; Wang, X.; Cui, P.; Jin, J.; Chen, J.; Zhang, W.; Zhang, Y.
UbiA (Rv3806c) encoding DPPR synthase involved in cell wall synthesis is associated with ethambutol resistance in Mycobacterium tuberculosis
Tuberculosis
95
149-154
2015
Mycobacterium tuberculosis (P9WFR5), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WFR5)
Manually annotated by BRENDA team