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Information on EC 2.4.2.40 - zeatin O-beta-D-xylosyltransferase and Organism(s) Phaseolus vulgaris and UniProt Accession P56725

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.40 zeatin O-beta-D-xylosyltransferase
IUBMB Comments
Does not act on UDP-glucose (cf. EC 2.4.1.103 alizarin 2-beta-glucosyltransferase).
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This record set is specific for:
Phaseolus vulgaris
UNIPROT: P56725
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The taxonomic range for the selected organisms is: Phaseolus vulgaris
The expected taxonomic range for this enzyme is: Phaseolus vulgaris
Synonyms
zeatin o-xylosyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
uridine diphosphoxylose-zeatin xylosyltransferase
-
-
-
-
xylosyltransferase, uridine diphosphoxylose-zeatin
-
-
-
-
zeatin O-xylosyltransferase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-D-xylose + zeatin = UDP + O-beta-D-xylosylzeatin
show the reaction diagram
localisation of site determining the substrate specificity for the UDP-sugar donors
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-D-xylose:zeatin O-beta-D-xylosyltransferase
Does not act on UDP-glucose (cf. EC 2.4.1.103 alizarin 2-beta-glucosyltransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
110541-22-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + zeatin
UDP + O-beta-D-xylosylzeatin
show the reaction diagram
absolutely specific for UDP-D-xylose
-
?
UDP-D-xylose + dihydrozeatin
UDP + O-beta-D-xylosyldihydrozeatin
show the reaction diagram
UDP-D-xylose + trans-zeatin
?
show the reaction diagram
-
may be involved in the nuclear-cytoplasmic transport of cytokinins and related molecules or possibly, with chromatin of rapidly dividing cells
-
-
?
UDP-D-xylose + zeatin
UDP + O-beta-D-xylosylzeatin
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + trans-zeatin
?
show the reaction diagram
-
may be involved in the nuclear-cytoplasmic transport of cytokinins and related molecules or possibly, with chromatin of rapidly dividing cells
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
UDP-glucose
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
dihydrozeatin
-
-
0.002
trans-zeatin
-
-
0.003
UDP-xylose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
27
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
immature
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ZOX_PHAVU
454
0
50972
Swiss-Prot
Secretory Pathway (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51000
DNA sequence determination
50000
additional information
-
an inactive form of MW about 70 kDa is detected by immunostaining in vegetative tissue prior to posttranslational processing of the protein
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 50000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ZOX1 gene, DNA sequence determination and analysis
expression in Escherichia coli and in Spodoptera frugiperda Sf21 cells, via baculovirus infection, produced an insoluble protein, not successful
-
in vitro transcription and translation, posttranslational processing by bean endosperm extract
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Martin, R.C.; Cloud, K.A.; Mok, M.C.; Mok, D.W.S.
Substrate specificity and domain analyses of zeatin O-glycosyltransferases
Plant Growth Regul.
32
289-293
2000
Phaseolus vulgaris
-
Manually annotated by BRENDA team
Turner, J.E.; Mok, D.W.S.; Mok, M.C.; Shaw, G.
Isolation and partial purification of an enzyme catalyzing the formation of O-xylosylzeatin in phaseolus vulgaris embryos
Proc. Natl. Acad. Sci. USA
84
3714-3717
1987
no activity in Phaseolus lunatus, Phaseolus vulgaris
Manually annotated by BRENDA team
Martin, R.C.; Mok, M.C.; Mok, D.W.S.
Cytolocalization of zeatin O-xylosyltransferase in Phaseolus
Proc. Natl. Acad. Sci. USA
90
953-957
1993
Phaseolus vulgaris, Phaseolus vulgaris x Phaseolus coccineus
Manually annotated by BRENDA team
Martin, R.C.; Mok, M.C.; Mok, D.W.S.
Protein processing and auxin response in transgenic tobacco harboring a putative cDNA of zeatin O-xylosyltransferase from Phaseolus vulgaris
Plant J.
12
305-312
1997
Phaseolus vulgaris
Manually annotated by BRENDA team
Martin, R.C.; Mok, M.C.; Mok, D.W.S.
A gene encoding the cytokinin enzyme zeatin O-xylosyltransferase of Phaseolus vulgaris
Plant Physiol.
120
553-557
1999
Phaseolus vulgaris (P56725), Phaseolus vulgaris
Manually annotated by BRENDA team