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Information on EC 2.4.2.39 - xyloglucan 6-xylosyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession O22775

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.39 xyloglucan 6-xylosyltransferase
IUBMB Comments
In association with EC 2.4.1.168 (xyloglucan 4-glucosyltransferase), this enzyme brings about the synthesis of xyloglucan; concurrent transfers of glucose and xylose are necessary for this synthesis.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O22775
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
xyloglucan alpha-1,6-xylosyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
xyloglucan alpha-1,6-xylosyltransferase
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xyloglucan 6-alpha-D-xylosyltransferase
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-
-
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xyloglucan alpha-1,6-xylosyltransferase
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xylosyltransferase, uridine diphosphoxylose-xyloglucan 6alpha-
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-D-xylose:xyloglucan 6-alpha-D-xylosyltransferase
In association with EC 2.4.1.168 (xyloglucan 4-glucosyltransferase), this enzyme brings about the synthesis of xyloglucan; concurrent transfers of glucose and xylose are necessary for this synthesis.
CAS REGISTRY NUMBER
COMMENTARY hide
80238-01-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
-
-
-
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
-
reduction of xyloglucan content (reduced glucan backbone substitution with xylose) in plants that lack functional XXT5 can be complemented by transformation with recombinant XXT5
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?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
high expression level, revealed by Reverse Transcription-PCR
Manually annotated by BRENDA team
expressed in all major organs, revealed by Reverse Transcription-PCR
Manually annotated by BRENDA team
high expression level, revealed by Reverse Transcription-PCR, shorter root hair and less root tissue xyloglucan detectable upon lack of XXT5
Manually annotated by BRENDA team
silique, lowest expression level, revealed by Reverse Transcription-PCR
Manually annotated by BRENDA team
high expression level, revealed by Reverse Transcription-PCR
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
complex formation in vivo and potential physical interactions among three xylosyltransferases, XXT1, XXT2, and XXT5, and a glucan synthase, CSLC4 is shown in the Golgi membrane in Arabidopsis cells
Manually annotated by BRENDA team
immunolocalization of recombinant enzyme using tag-specific antibody, predicted Golgi-resident type-II membrane protein
Manually annotated by BRENDA team
complex formation in vivo and potential physical interactions among three xylosyltransferases, XXT1, XXT2, and XXT5, and a glucan synthase, CSLC4 is shown in the Golgi membrane in Arabidopsis cells
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
double mutants for either xxt2 or xxt5 have a large impact on XyG content, structure and size distribution
malfunction
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
XXT2_ARATH
461
1
53095
Swiss-Prot
Mitochondrion (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51000
predicted from primary structure
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
existence of heterocomplexes XXT2-XXT5 and XXT1-XXT2 are demonstrated
homodimer
existence of homocomplex XXT2-XXT2 is shown
heterodimer
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a truncated protein lacking the N-terminal transmembrane domain as GST-fusion protein
expressed in Escherichia coli as a truncated protein lacking the N-terminal transmembrane domain as GST-fusion protein
in pENTR/D-TOPO downstream of cauliflower mosaic virus 35S promoter for recombination with pEarleyGate 201 and Agrobacterium tumefacies strain GV3101-mediated transformation (floral-flip), expression with hemagglutinin (HA) tag
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zabotina, O.A.; van de Ven, W.T.; Freshour, G.; Drakakaki, G.; Cavalier, D.; Mouille, G.; Hahn, M.G.; Keegstra, K.; Raikhel, N.V.
Arabidopsis XXT5 gene encodes a putative alpha-1,6-xylosyltransferase that is involved in xyloglucan biosynthesis
Plant J.
56
101-115
2008
Arabidopsis thaliana (Q9CA75), Arabidopsis thaliana
Manually annotated by BRENDA team
Vuttipongchaikij, S.; Brocklehurst, D.; Steele-King, C.; Ashford, D.A.; Gomez, L.D.; McQueen-Mason, S.J.
Arabidopsis GT34 family contains five xyloglucan alpha-1,6-xylosyltransferases
New Phytol.
195
585-595
2012
Arabidopsis thaliana (O22775), Arabidopsis thaliana (Q9CA75), Arabidopsis thaliana (Q9LJP4), Arabidopsis thaliana (Q9LZJ3)
Manually annotated by BRENDA team
Chou, Y.H.; Pogorelko, G.; Zabotina, O.A.
Xyloglucan xylosyltransferases XXT1, XXT2, and XXT5 and the glucan synthase CSLC4 form Golgi-localized multiprotein complexes
Plant Physiol.
159
1355-1366
2012
Arabidopsis thaliana (O22775), Arabidopsis thaliana (Q9CA75), Arabidopsis thaliana (Q9LZJ3)
Manually annotated by BRENDA team