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Information on EC 2.4.2.31 - NAD+-protein-arginine ADP-ribosyltransferase
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2.4.2.31 NAD+-protein-arginine ADP-ribosyltransferaseIUBMB Comments
Protein mono-ADP-ribosylation is a reversible post-translational modification that plays a role in the regulation of cellular activities . Arginine residues in proteins act as acceptors. Free arginine, agmatine [(4-aminobutyl)guanidine], arginine methyl ester and guanidine can also do so. The enzyme from some, but not all, species can also use NADP+ as acceptor (giving rise to Nomega-[(2'-phospho-ADP)-D-ribosyl]-protein-L-arginine as the product), but more slowly [1,5]. The enzyme catalyses the NAD+-dependent activation of EC 4.6.1.1, adenylate cyclase. Some bacterial enterotoxins possess similar enzymic activities. (cf. EC 2.4.2.36 NAD+---diphthamide ADP-ribosyltransferase).
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Word Map
- 2.4.2.31
- angiotensin
- losartan
- arterial
-
renin-angiotensin
- hypertension
- subtype
-
blocker
- cardiovascular
- cardiac
- ventricular
- renin
- hypertrophy
-
ii-induced
-
angiotensin-converting
- agonist
-
hemodynamic
-
vasoconstriction
- valsartan
- bradykinin
-
angii
- candesartan
-
sympathetic
- angiotensinogen
-
antihypertensive
- aldosterone
-
nonpeptide
-
pressor
- aortic
- myocardial
-
systolic
-
normotensive
-
angii-induced
- medicine
- agriculture
- saralasin
- enalapril
-
aceis
-
conscious
- captopril
-
wistar-kyoto
-
baroreflex
-
renin-angiotensin-aldosterone
-
subfornical
-
natriuresis
-
ii-stimulated
-
angiotensin-1-7
-
intrarenal
- telmisartan
- angiotensin-ii
- olmesartan
-
ii-mediated
- irbesartan
The enzyme appears in viruses and cellular organisms
Synonyms
at1, at2, iota toxin, rt6.1, rt6.2, mono(adp-ribosyl)transferase, arginine-specific adp-ribosyltransferase, nad:arginine adp-ribosyltransferase, arginine-specific mono-adp-ribosyltransferase, artase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(adenosine diphosphoribose)transferase, nicotinamide adenine dinucleotide-arginine
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ADP-ribosyltransferase
ADPRT
alloantigen Rt6.1
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alloantigen Rt6.2
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arginine ADP-ribosyltransferase 1
arginine specific ADP-ribosyltransferase
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arginine specific mono-ADP-ribosyltransferase
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arginine-specific ADP-ribosyltransferase
arginine-specific mono-ADP-ribosyltransferase
ART
ART1
ART4
AT1
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AT2
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cARTC2.1
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bi-functional: NAD-glycohydrolase and arginine-specific ADP-ribosyltransferase activities
CD196 ADP-ribosyltransferase
CDT
CdtA
cholera toxin
Dombrock blood group carrier molecule
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glycosylphosphatidylinositol-anchored arginine-specific ADP-ribosyltransferase7.1
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iota toxin
mono(ADP-ribosyl)transferase
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mono-ADP-ribosyltransferase
NAD(P)+-arginine ADP-ribosyltransferase
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NAD+:arginine ADP-ribosyltransferase
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NAD+:arginine ecto-mono(ADP-ribosyl)transferase
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NAD+:L-arginine ADP-D-ribosyltransferase
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NAD-arginine ADP-ribosyltransferase
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NAD-arginine mono-ADP-ribosyltransferase B
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NAD-dependent ADPribosyltransferase
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NAD:arginine ADP-ribosyltransferase B
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NarE
RT6
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RT6.1
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RT6.2
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T-cell surface protein Rt6.1
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T-cell surface protein Rt6.2
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VIP2
XAC3230
XopAI
ADP-ribosyltransferase
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ADPRT
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arginine-specific mono-ADP-ribosyltransferase
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ART
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
NAD+ + L-arginine = nicotinamide + Nomega-(ADP-D-ribosyl)-L-arginine + H+
rapid equilibrium random sequential mechanism
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NAD+ + L-arginine = nicotinamide + Nomega-(ADP-D-ribosyl)-L-arginine + H+
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pentosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
NAD+:protein-L-arginine ADP-D-ribosyltransferase
Protein mono-ADP-ribosylation is a reversible post-translational modification that plays a role in the regulation of cellular activities [4]. Arginine residues in proteins act as acceptors. Free arginine, agmatine [(4-aminobutyl)guanidine], arginine methyl ester and guanidine can also do so. The enzyme from some, but not all, species can also use NADP+ as acceptor (giving rise to Nomega-[(2'-phospho-ADP)-D-ribosyl]-protein-L-arginine as the product), but more slowly [1,5]. The enzyme catalyses the NAD+-dependent activation of EC 4.6.1.1, adenylate cyclase. Some bacterial enterotoxins possess similar enzymic activities. (cf. EC 2.4.2.36 NAD+---diphthamide ADP-ribosyltransferase).
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CAS REGISTRY NUMBER
COMMENTARY
81457-93-4
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
A1 peptide of cholera toxin + NAD+
nicotinamide + ?
-
four mol of ADP-ribose are incorporated into 1 mol of A1 peptide. Modification of the A1 peptide increases its enzymatic activity up to 4fold
-
-
?
bovine serum albumin + NAD+
nicotinamide + ?
-
poor ADP-ribose acceptor
-
-
?
histone + NAD+
Nomega-[(2'-phospho-ADP)-D-ribosyl]-histone-L-arginine + nicotinamide + H+
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isozyme ART2 is active with high and low molecular weight histones, membrane-bound and PI-PLC released ART2 preferentially ADP-ribosylate high or low MW histones, respectively
-
-
?
histone H2B + NAD+
Nomega-[(2'-phospho-ADP)-D-ribosyl]-histone H2B-L-arginine + nicotinamide
Meleagris sp.
-
-
-
-
?
human neutrophil peptide-1 + NAD+
Nomega-(ADP-D-ribosyl)-L-arginine-human neutrophil peptide-1 + nicotinamide
L-arginine methyl ester + NADP+
nicotinamide + N2-(2'-phospho-ADP-D-ribosyl)-L-arginine
-
-
-
-
?
NAD+ + (ADP-D-ribosyl)n-acceptor
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
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agmatine as ADPribose acceptor is used
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-
?
NAD+ + alpha-actin-L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-alpha-actin-L-arginine
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skeletal muscle alpha actin
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-
?
NAD+ + beta-actin
nicotinamide + H+ + (ADP-ribosyl)-beta-actin
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modification occurs at residue Arg177
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?
NAD+ + beta/gamma-actin-L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-beta/gamma-actin-L-arginine
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non-muscle beta/gamma actin
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-
?
NAD+ + eEF2 L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-eEF2-L-arginine
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-
-
?
NAD+ + gamma-actin
nicotinamide + H+ + (ADP-ribosyl)-gamma-actin
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modification occurs at residue Arg177
-
?
NAD+ + gamma-actin-L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-gamma-actin-L-arginine
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smooth muscle gamma actin
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-
?
NAD+ + glucose-regulated protein of 78 kDa/immunoglobulin heavy-chainbinding protein L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-glucose-regulated protein of 78 kDa/immunoglobulin heavy-chainbinding protein-L-arginine
-
-
-
?
NAD+ + human neutrophil peptide-1
nicotinamide + Nomega-(ADP-D-ribosyl)-human neutrophil peptide-1
NAD+ + MazF L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-MazF-L-arginine
Tequatrovirus T4
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the enzyme ADP-ribosylates MazF at an arginine residue at position 4
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-
?
NAD+ + muscle actin
nicotinamide + H+ + (ADP-ribosyl)-muscle actin
substrate source rabbit skeletal muscle
modification occurs at residue Arg177
-
?
NAD+ + non-muscle actin from human platelets
nicotinamide + (ADP-D-ribosyl)-non-muscle actin from human platelets
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-
-
-
?
NAD+ + polyarginine
nicotinamide + Nomega-(ADP-D-ribosyl)-polyarginine
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-
-
?
NAD+ + protein poly-L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-protein-poly-L-arginine
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?
NAD+ + protein R10H6 L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-protein N10H6-L-arginine
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-
-
?
NAD+ + rabbit muscle actin
nicotinamide + (ADP-D-ribosyl)-rabbit muscle actin
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-
-
?
nicotinamide + Nomega-(ADP-D-ribosyl)-agmatine-L-arginine
NAD+ + agmatine L-arginine
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r
p-nitrobenzylidine aminoguanidine + NAD+
nicotinamide + mono-ADP-ribosylated p-nitrobenzylidine aminoguanidine
P2X7 purinoceptor protein + NAD+
Nomega-[(2'-phospho-ADP)-D-ribosyl]-P2X7 purinoceptor protein-L-arginine + nicotinamide
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isozyme ART2
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-
?
P2X7(k) + NAD+
nicotinamide + ?
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the P2X7(k) variant is sensitive to activation by ADP-ribosylation whereas the P2X7(a) variant is insensitive
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-
?
Ras + NAD+
nicotinamide + ?
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ADP-ribosylation of Ras at Arg41 and Arg 128. The double mutant RasR141K/R128K is ribosylated at an alternative sitem Arg135
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-
?
RhoA + NAD+
Nomega-(ADP-D-ribosyl)-L-asparagine41-RhoA + nicotinamide
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the enzyme modifies the low-molecular-mass GTPase RhoA specifically at Asn41
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-
?
RhoB + NAD+
Nomega-(ADP-D-ribosyl)-L-asparagine41-RhoB + nicotinamide
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the enzyme modifies the low-molecular-mass GTPase RhoB specifically at Asn41
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-
?
RhoC + NAD+
Nomega-(ADP-D-ribosyl)-L-asparagine41-RhoC + nicotinamide
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the enzyme modifies the low-molecular-mass GTPase RhoC specifically at Asn41
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-
?
soybean trypsin inhibitor + NAD+
Nomega-(ADP-D-ribosyl)-L-arginine-soybean trypsin inhibitor + nicotinamide
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?
wild-type exoenzyme C3 + NAD+
Nomega-(ADP-D-ribosyl)-L-arginine86-wild-type exoenzyme C3 + nicotinamide
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the recombinant mutant Q217E enzyme modifies the recombinant wild-type enzyme at Arg86
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-
?
actin + NAD+
nicotinamide + ?
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non-muscle beta/gamma actin, skeletal muscle alpha actin, smooth muscle gamma actin
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-
?
actin + NAD+
nicotinamide + ?
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the ADP ribosylation of actin in the heterophils may be involved in the cellular processes such as phagocytosis, secretion and migration
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-
?
actin + NAD+
nicotinamide + ?
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no activity with the enzyme from skeletal muscle sarcoplasmic reticulum
-
-
?
FGF-2 + NAD+
?
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ADP-ribosylation of FGF-2 may provide an additional level of control of FGF-2 activity
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-
?
FGF-2 + NAD+
?
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ADP-ribosylation of FGF-2 may provide an additional level of control of FGF-2 activity
-
-
?
histone + NAD+
nicotinamide + ?
130% of the activity with p33
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-
?
histone + NAD+
nicotinamide + ?
67% of the activity with p33
-
-
?
human alpha-defensin-1 + NAD+
nicotinamide + ?
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poorly recognised substrate
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-
?
human neutrophil peptide-1 + NAD+
Nomega-(ADP-D-ribosyl)-L-arginine-human neutrophil peptide-1 + nicotinamide
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human neutrophil peptide-1, HNP-1, from neutrophil cell surface, ADP-ribosylation of HNP-1 is primarily an activity of ART1 and occurs in inflammatory conditions and disease, lack of modified HNP-1 due to enzyme deficiency in the sputum leads to cystic fibrosis
-
-
?
human neutrophil peptide-1 + NAD+
Nomega-(ADP-D-ribosyl)-L-arginine-human neutrophil peptide-1 + nicotinamide
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human neutrophil peptide-1, HNP-1, from neutrophil cell surface, isozyme ART1 shows high activity on Arg14 and Arg24, while isozymes ART3, ART4, ART5 are less or not active
-
-
?
human neutrophil peptide-1 + NAD+
Nomega-(ADP-D-ribosyl)-L-arginine-human neutrophil peptide-1 + nicotinamide
-
human neutrophil peptide-1, HNP-1, from neutrophil cell surface, isozyme ART1 is active on Arg14 and Arg24
-
-
?
L-arginine methyl ester + NAD+
nicotinamide + Nomega-(ADP-D-ribosyl)-L-arginine
-
-
-
?
L-arginine methyl ester + NAD+
nicotinamide + Nomega-(ADP-D-ribosyl)-L-arginine
-
-
-
-
?
NAD+ + actin
nicotinamide + H+ + (ADP-ribosyl)-actin
-
Clostridium botulinum C2 toxin ADP-ribosylates actin isoforms gizzard gamma-smooth muscle actin, spleen beta- and gamma-cytoplasmic actin, and gamma-smooth muscle actin isoforms of aortic smooth muscle actin
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-
?
NAD+ + actin
nicotinamide + H+ + (ADP-ribosyl)-actin
Clostridium perfringens iota toxin ADP-ribosylates all actin isoforms tested, i.e. alpha-skeletal muscle actin, alpha-cardiac muscle actin, gizzard gamma-smooth muscle actin, spleen beta- and gamma-cytoplasmic actin, and aortic smooth muscle actin containing alpha- and gamma-smooth muscle actin isoforms
-
-
?
NAD+ + agmatine
nicotinamide + Nomega-(ADP-D-ribosyl)-agmatine
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the enzyme labels L-arginine with ADP-ribose from the NAD(+) substrate at the amino nitrogen of the guanidinium side chain
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-
r
NAD+ + human neutrophil peptide-1
nicotinamide + Nomega-(ADP-D-ribosyl)-human neutrophil peptide-1
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the enzyme ADP-ribosylates human neutrophil peptide-1 on arginine 14 and 24
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-
?
NAD+ + human neutrophil peptide-1
nicotinamide + Nomega-(ADP-D-ribosyl)-human neutrophil peptide-1
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the enzyme ADP-ribosylates human neutrophil peptide-1 on arginine 14 and 24
-
-
?
NAD+ + protein L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine
-
-
-
-
?
NAD+ + protein L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine
-
-
-
-
?
NAD+ + protein L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine
-
-
-
?
NAD+ + protein L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine
-
-
-
-
?
NAD+ + protein L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine
-
-
-
-
?
NAD+ + protein L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine
-
-
-
?
NAD+ + protein L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine
-
-
-
?
NAD+ + protein L-arginine
nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine
-
-
-
?
NAD+ + [actin]-L-arginine177
nicotinamide + [actin]-N-(ADP-D-ribosyl)-L-arginine177 + H+
-
-
-
?
NAD+ + [actin]-L-arginine177
nicotinamide + [actin]-N-(ADP-D-ribosyl)-L-arginine177 + H+
-
-
-
-
?
NAD+ + [actin]-L-arginine177
nicotinamide + [actin]-N-(ADP-D-ribosyl)-L-arginine177 + H+
-
-
-
-
?
NAD+ + [actin]-L-arginine177
nicotinamide + [actin]-N-(ADP-D-ribosyl)-L-arginine177 + H+
-
-
-
-
?
NAD+ + [actin]-L-arginine177
nicotinamide + [actin]-N-(ADP-D-ribosyl)-L-arginine177 + H+
-
-
-
?
NAD+ + [actin]-L-arginine177
nicotinamide + [actin]-N-(ADP-D-ribosyl)-L-arginine177 + H+
-
-
-
-
?
p-nitrobenzylidine aminoguanidine + NAD+
nicotinamide + mono-ADP-ribosylated p-nitrobenzylidine aminoguanidine
-
-
-
?
p-nitrobenzylidine aminoguanidine + NAD+
nicotinamide + mono-ADP-ribosylated p-nitrobenzylidine aminoguanidine
-
-
-
?
P33 + NAD+
nicotinamide + ?
-
no activity with the enzyme from skeletal muscle sarcoplasmic reticulum
-
-
?
polyarginine + NAD+
nicotinamide + ?
in contrast to its mammalian orthologues, the chicken protein contains an intact R-S-EXE motif
-
-
?
additional information
?
-
-
no substrates: alpha-skeletal muscle actin, alpha-smooth muscle actin or alpha-cardiac muscle actin. The N-terminal region of actin isoforms define the substrate specificity for ADP-ribosylation by Clostridium botulinum C2 toxin
-
-
?
additional information
?
-
in absence of actin, enzyme displays weak NAD+ glycohydrolase activity
-
-
?
additional information
?
-
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the enzyme exhibits ADP-ribosyltransferase and NAD+ glycohydrolase activity, modeling of interaction od substrate and catalytic residues, overview
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-
?
additional information
?
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enzyme is involved in posttranslational modification of proteins
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-
?
additional information
?
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substrate specificity, beta-defensin-1 is a poor substrate, overview