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Information on EC 2.4.1.B72 - C-mannosyltransferase and Organism(s) Homo sapiens and UniProt Accession Q6ZPD9

for references in articles please use BRENDA:EC2.4.1.B72
preliminary BRENDA-supplied EC number
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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.B72 C-mannosyltransferase
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This record set is specific for:
Homo sapiens
UNIPROT: Q6ZPD9 not found.
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
transfers a D-mannosyl residue from dolichyl D-mannosyl phosphate to the tryptophan residue of a protein acceptor forming a C-C bond
Synonyms
dpy19l1, dpy19l3, dpy-19, c-mannosyltransferase, dpy19, dpy19l4, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C-mannosyltransferase
-
Dpy-19-like protein 3
UniProt
protein dpy-19 homolog 3
UniProt
SYSTEMATIC NAME
IUBMB Comments
dolichyl-D-mannosyl-phosphate:protein C-mannosyltransferase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
a multipass transmembrane protein. DPY19L3 comprises 11 transmembrane regions and two re-entrant loops with the N- and C-terminal ends facing the cytoplasm and ER lumen, respectively. Determination of ER membrane topology of DPY19L3 by redox-sensitive luciferase (Gaussia luciferase, Gluc) assay, overview
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
knockdown of DPY19L3 inhibits the secretion of Rspo1. Lec15.2 cells lack dolichol-phosphate-mannose synthesis activity
physiological function
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
D19L3_HUMAN
716
0
83197
Swiss-Prot
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
DPY19L3 has four predicted N-glycosylation sites, DPY19L3 is N-glycosylated at Asn118 and Asn704 but not Asn319 and Asn439. N-glycosylations of DPY19L3 do not play any roles for its enzymatic activity
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E206N
site-directed mutatgenesis, the mutation of DPY19L3, resulting in the N-glycosylation consensus sequence (206NFT208), does not induce N-glycosylation, confirming the region is certainly a cytoplasmic region
N118Q
site-directed mutagenesis of the N-glycosylation site
N118Q/N704Q
site-directed mutagenesis of N-glycosylation sites
N319Q
site-directed mutagenesis of a potential N-glycosylation site
N439Q
site-directed mutagenesis of a potential N-glycosylation site
N704Q
site-directed mutagenesis of the N-glycosylation site
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme, coexpressed with substrate Rspo1, by heparin and nickel affinity chromatography
recombinant wild-type and mutant enzymes from Drosophila melanogaster S2 cells by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant expression in S2 cells, coexpression with substrate Rspo1
transient recombinant expression of wild-type and mutant enzymes in Drosophila melanogaster S2 cells or HEK-293T cells fused to Gaussia luciferase or tagged with myc or myc-His6 tags, quantitative RT-PCR expression analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Niwa, Y.; Suzuki, T.; Dohmae, N.; Simizu, S.
Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells
Mol. Biol. Cell
27
744-756
2016
Homo sapiens (Q6ZPD9), Homo sapiens
Manually annotated by BRENDA team
Niwa, Y.; Nakano, Y.; Suzuki, T.; Yamagishi, M.; Otani, K.; Dohmae, N.; Simizu, S.
Topological analysis of DPY19L3, a human C-mannosyltransferase
FEBS J.
285
1162-1174
2018
Homo sapiens (Q6ZPD9), Homo sapiens
Manually annotated by BRENDA team