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Information on EC 2.4.1.88 - globoside alpha-N-acetylgalactosaminyltransferase and Organism(s) Homo sapiens and UniProt Accession P16442

for references in articles please use BRENDA:EC2.4.1.88
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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.88 globoside alpha-N-acetylgalactosaminyltransferase
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This record set is specific for:
Homo sapiens
UNIPROT: P16442 not found.
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
gbgt1, forssman synthase, gt6m6, gt6m7, forssman synthetase, gt6m5, globoside alpha-1,3-n-acetylgalactosaminyltransferase 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-globoside alpha-
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Forssman synthase
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Forssmann glycolipid synthetase
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globoside acetylgalactosaminyltransferase
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globoside alpha-1,3-N-acetylgalactosaminyltransferase 1
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uridine diphosphoacetylgalactosamine-globoside alpha-acetylgalactosaminyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide alpha-N-acetyl-D-galactosaminyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
52037-97-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosylceramide
UDP + N-acetyl-D-galactosaminyl-(1->3)-N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosylceramide
show the reaction diagram
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-
?
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
show the reaction diagram
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cf. EC 2.4.1.37, EC 2.4.1.40
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
a normal ovarian surface epithelium cell line
Manually annotated by BRENDA team
a normal ovarian surface epithelium cell line
Manually annotated by BRENDA team
cDNA library
Manually annotated by BRENDA team
primary or cell culture
Manually annotated by BRENDA team
high enzyme expression level
Manually annotated by BRENDA team
high enzyme expression level
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the deletion of exon 3 or 4, but not of exon 2 or 5, of human GTA transcripts bestows moderate Forssman glycolipid synthase activity. Strong Forssman glycolipid synthase activity is attained, in addition to GTA and GTB activities, by cointroducing one of those deletions and the replacement of the LeuGlyGly tripeptide sequence at codons 266 to 268 by GlyGlyAla substitution, i.e. to the residues of Forssman glycolipid synthase
malfunction
GBGT1 expression is epigenetically silenced through promoter hypermethylation in ovarian cancer
metabolism
the enzyme catalyzes the last step in the multi-step biosynthesis of the Forssman antigen, Fs antigen, a pentaglycosyl ceramide of the globo series glycosphingolipids. Involvement of DNA methylation in the synthesis of Fs antigen
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BGAT_HUMAN
354
1
40934
Swiss-Prot
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in COS-1 cell
cloned from cDNA library by PCR, expression, transcription and processing, of wild-type in COS-1 cells with and without HA-tag, but no detectable enzyme activity in the transfected cells by recombinant enzyme, DNA and amino acid sequence determination and analysis
Gb4-synthase, expression in 1B9 mouse fibroblast L cells, coexpression of SV 40 large T antigen for extrachromosomal replication of the transfected plasmids
gene GBGT1, quantitative RT-PCR enzyme expression profiling, high or intermediate levels of GBGT1 DNA methylation in all ovarian cancer cell lines (except for OVCAR3) but marginal levels of DNA methylation in the two normal ovary surface epithelial cell lines
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
GBGT1 expression is epigenetically silenced through promoter hypermethylation in ovarian cancer. The extent of DNA methylation inversely correlates with GBGT1 mRNA and protein expression
restoration of GBGT1 mRNA and protein expression in low GBGT1-expressing A2780 cells is achieved by 5-aza-2'-deoxycytidine treatment
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Okajima, T.; Nakamura, Y.; Uchikawa, M.; Haslam, D.B.; Numata, S.I.; Furukawa, K.; Urano, T.; Furukawa, K.
Expression cloning of human globoside synthase cDNAs. Identification of beta 3Gal-T3 as UDP-N-acetylgalactosamine:globotriaosylceramide beta 1,3-N-acetylgalactosaminyltransferase
J. Biol. Chem.
275
40498-40503
2000
Homo sapiens (O75752), Homo sapiens
Manually annotated by BRENDA team
Xu, H.; Storch, T.; Yu, M.; Elliott, S.P.; Haslam, D.B.
Characterization of the human Forssman synthetase gene. An evolving association between glycolipid synthesis and host-microbial interactions
J. Biol. Chem.
274
29390-29398
1999
Canis lupus familiaris, Homo sapiens, Homo sapiens (Q8N5D6)
Manually annotated by BRENDA team
Jacob, F.; Hitchins, M.P.; Fedier, A.; Brennan, K.; Nixdorf, S.; Hacker, N.F.; Ward, R.; Heinzelmann-Schwarz, V.A.
Expression of GBGT1 is epigenetically regulated by DNA methylation in ovarian cancer cells
BMC Mol. Biol.
15
24
2014
Homo sapiens (Q8N5D6), Homo sapiens
Manually annotated by BRENDA team
Yamamoto, M.; Tarasco, M.C.; Cid, E.; Kobayashi, H.; Yamamoto, F.
ABO blood group A transferase and its codon 69 substitution enzymes synthesize FORS1 antigen of FORS blood group system
Sci. Rep.
9
9717
2019
Homo sapiens (P16442), Homo sapiens
Manually annotated by BRENDA team