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Information on EC 2.4.1.67 - galactinol-raffinose galactosyltransferase and Organism(s) Pisum sativum and UniProt Accession Q93XK2

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.67 galactinol-raffinose galactosyltransferase
IUBMB Comments
This enzyme also catalyses galactosyl transfer from stachyose to raffinose (shown by labelling) . For synthesis of the substrate, see EC 2.4.1.123, inositol 3-alpha-galactosyltransferase. See also EC 2.4.1.82, galactinol---sucrose galactosyltransferase.
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This record set is specific for:
Pisum sativum
UNIPROT: Q93XK2
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Word Map
The taxonomic range for the selected organisms is: Pisum sativum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
stachyose synthase, amsts1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
stachyose synthase
-
galactosyltransferase, galactinol-raffinose
-
-
-
-
stachyose synthetase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-D-galactosyl-(1->3)-1D-myo-inositol + raffinose = myo-inositol + stachyose
show the reaction diagram
minimum double displacement mechanism
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-D-galactosyl-(1->3)-1D-myo-inositol:raffinose galactosyltransferase
This enzyme also catalyses galactosyl transfer from stachyose to raffinose (shown by labelling) [4]. For synthesis of the substrate, see EC 2.4.1.123, inositol 3-alpha-galactosyltransferase. See also EC 2.4.1.82, galactinol---sucrose galactosyltransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
37277-70-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-alpha-D-galactosyl-myo-inositol + D-ononitol
myo-inositol + alpha-D-galactosyl-D-ononitol
show the reaction diagram
1-alpha-D-galactosyl-myo-inositol + D-pinitol
myo-inositol + galactopinitol A
show the reaction diagram
1-alpha-D-galactosyl-myo-inositol + H2O
myo-inositol + D-galactose
show the reaction diagram
hydrolysis
-
?
1-alpha-D-galactosyl-myo-inositol + raffinose
myo-inositol + stachyose
show the reaction diagram
i.e. galactinol
-
?
1-alpha-D-galactosyl-myo-inositol + sequoyitol
myo-inositol + alpha-D-galactosyl-sequoyitol
show the reaction diagram
1-alpha-D-galactosyl-myo-inositol + stachyose
myo-inositol + verbascose
show the reaction diagram
12.1% activity compared to raffinose
-
?
1-alpha-D-galactosyl-myo-inositol + verbascose
myo-inositol + ajugose
show the reaction diagram
-
-
?
alpha-D-galactosyl-D-ononitol + raffinose
D-ononitol + stachyose
show the reaction diagram
65.8% activity compared to 1-alpha-D-galactosyl-myo-inositol
-
?
alpha-D-galactosyl-D-ononitol + stachyose
D-ononitol + verbascose
show the reaction diagram
9.9% activity compared to 1-alpha-D-galactosyl-myo-inositol
-
?
stachyose + stachyose
raffinose + verbascose
show the reaction diagram
-
-
?
additional information
?
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.5
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cv. Wunder von Kelvedon
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
developing
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
STSYN_PEA
853
0
95890
Swiss-Prot
Chloroplast (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
95000
1 * 95000, about, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 95000, about, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned from maturing seeds, DNA sequence determination and analysis, functional expression in Spodoptera frugiperda Sf9 insect cells via baculovirus infection
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Peterbauer, T.; Mucha, J.; Mach, L.; Richter, A.
Chain Elongation of raffinose in pea seeds. Isolation, characterization, and molecular cloning of mutifunctional enzyme catalyzing the synthesis of stachyose and verbascose
J. Biol. Chem.
277
194-200
2002
Pisum sativum (Q93XK2), Pisum sativum
Manually annotated by BRENDA team