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Information on EC 2.4.1.64 - alpha,alpha-trehalose phosphorylase
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2.4.1.64 alpha,alpha-trehalose phosphorylaseSpecify your search results
Word Map
- 2.4.1.64
-
phosphorolysis
- trehalase
- 1-phosphate
- phosphorylases
- thermoanaerobacter
- schizophyllum
- brockii
- alpha-d-glucose
- trehalose-6-phosphate
-
alpha-glucose-1-phosphate
- kojibiose
-
alpha-retaining
-
alpha-d-glucopyranosyl
-
enzyme-phosphate
- synthesis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
trehalose phosphorylase, bsel_1207, alpha,alpha-trehalose phosphorylase, inverting trehalose phosphorylase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Bsel_1207
phosphorylase, trehalose
-
-
-
-
trehalose phosphorylase
trehalose phosphorylase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
alpha,alpha-trehalose + phosphate = D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
alpha,alpha-trehalose:phosphate beta-D-glucosyltransferase
-
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CAS REGISTRY NUMBER
COMMENTARY
37205-59-7
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-deoxy-D-glucose + alpha-D-glucose 1-phosphate
alpha-D-glucopyranosyl 6-deoxy-alpha-D-glucopyranoside + phosphate
-
at 93% the activity with rate of glucose glucosylation
-
?
alpha,alpha-trehalose + phosphate
alpha-D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
?
alpha-D-galactopyranosyl alpha-D-glucopyranoside + phosphate
D-glucose + beta-D-galactose 1-phosphate
-
while the wild type enzyme shows very low activity, the mutant enzyme L649G/A693Q/W371Y displays a strict specificity (99%) for beta-D-galactose 1-phosphate as product
-
-
r
beta-D-glucose 1-phosphate + alpha-D-allose
?
-
4.5% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-D-arabinose
?
-
1.8% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-D-fucose
?
-
22% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-D-galactose
?
-
83.7% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-D-lyxose
?
-
1.3% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-D-mannose
?
-
14.1% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-D-xylose
?
-
135.2% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + alpha-L-fucose
?
-
109.2% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + beta-L-arabinose
?
-
79.9% activity compared to D-glucose
-
-
r
beta-D-glucose 1-phosphate + D-galactose
? + phosphate
low activity. The Km value for D-galactose is about 30fold higher than that for D-glucose
-
-
r
beta-D-glucose 1-phosphate + D-glucose
alpha,alpha-trehalose + phosphate
-
100% activity with D-glucose
-
-
r
beta-D-glucose-1-phosphate + gentiobiose
6-O-beta-D-glucopyranosyl trehalose + phosphate
-
-
-
-
?
beta-D-glucose-1-phosphate + isomaltose
6-O-alpha-D-glucopyranosyl trehalose + phosphate
-
-
-
-
?
beta-D-glucose-1-phosphate + melibiose
6-O-alpha-D-galactopyranosyl trehalose + phosphate
-
-
-
-
?
D-galactose + beta-D-glucose 1-phosphate
lactotrehalose + phosphate
-
-
-
-
r
D-glucose + beta-D-galactose 1-phosphate
alpha-D-galactopyranosyl alpha-D-glucopyranoside + phosphate
-
-
-
-
r
lactotrehalose + phosphate
D-galactose + beta-D-glucose 1-phosphate
-
-
-
-
r
xylose + beta-D-glucose 1-phosphate
glucosyl-1,1-xylose 1-phosphate
-
glucosylated at 23% of the rate of glucose glucosylation
-
?
alpha,alpha-trehalose + phosphate
beta-D-glucose 1-phosphate + D-glucose
-
specific substrate
-
-
r
alpha,alpha-trehalose + phosphate
beta-D-glucose 1-phosphate + D-glucose
-
-
-
-
r
alpha,alpha-trehalose + phosphate
beta-D-glucose 1-phosphate + D-glucose
Thermoanaerobacter brockii subsp. brockii ATCC 33075
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
enzyme is involved in trehalose catabolism
-
-
?
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
Salisediminibacterium selenitireducens
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
Salisediminibacterium selenitireducens MLS10
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
?
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
-
-
-
r
D-glucose + beta-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
Salisediminibacterium selenitireducens
-
-
-
r
D-glucose + beta-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
Salisediminibacterium selenitireducens MLS10
-
-
-
r
D-glucose + beta-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
best substrate combination
-
-
r
additional information
?
-
-
the enzyme shows no measurable activity with alpha-D-glucosamine, alpha-D-ribose, D-maltose or D-kojibiose
-
-
?
additional information
?
-
the wild type enzyme shows low activity with D-fructose and intermediate activity with D-mannose
-
-
?
additional information
?
-
-
the wild type enzyme shows low activity with D-fructose and intermediate activity with D-mannose
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-galactose + beta-D-glucose 1-phosphate
lactotrehalose + phosphate
-
-
-
-
r
lactotrehalose + phosphate
D-galactose + beta-D-glucose 1-phosphate
-
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
-
enzyme is involved in trehalose catabolism
-
-
?
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
Salisediminibacterium selenitireducens
-
-
-
r
alpha,alpha-trehalose + phosphate
D-glucose + beta-D-glucose 1-phosphate
Salisediminibacterium selenitireducens MLS10
-
-
-
r
D-glucose + beta-D-glucose 1-phosphate
alpha,alpha-trehalose + phosphate
Salisediminibacterium selenitireducens
-
-
-
r
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
D-glucose
-
strong substrate inhibition, 500 mM D-glucose reduces the enzyme activity to 49.1% of the activity at the saturating concentration of 30 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.86
alpha,alpha-trehalose
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
64.8
alpha-D-arabinose
-
in 50 mM MES buffer (pH 6.0), at 60°C
171.5
alpha-D-galactose
-
in 50 mM MES buffer (pH 6.0), at 60°C
20.3
alpha-D-xylose
-
in 50 mM MES buffer (pH 6.0), at 60°C
183.4
alpha-L-fucose
-
in 50 mM MES buffer (pH 6.0), at 60°C
0.7
beta-D-galactose 1-phosphate
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
3.8
beta-D-galactose 1-phosphate
-
wild type enzyme, at pH 6.0 and 60°C
0.7
beta-D-glucose 1-phosphate
-
wild type enzyme, at pH 6.0 and 60°C
2
beta-D-glucose 1-phosphate
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
3.1
beta-D-glucose 1-phosphate
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
3 - 3.5
D-galactose
mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C
30.1
D-galactose
mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C
35.1
D-galactose
mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
38
D-galactose
mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
43.7
D-galactose
mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C
43.8
D-galactose
mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C
45.2
D-galactose
mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C
45.7
D-galactose
mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C
45.8
D-galactose
mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
47.5
D-galactose
mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C
53.5
D-galactose
mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C
53.6
D-galactose
mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C
56.8
D-galactose
mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C
58
D-galactose
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
58.1
D-galactose
mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C
61.1
D-galactose
mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C
61.9
D-galactose
mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C
62.5
D-galactose
mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C
68.2
D-galactose
mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C
96
D-galactose
mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C
98
D-galactose
wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C
447.6
D-galactose
mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C
0.6
D-glucose
mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
0.6
D-glucose
mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
0.7
D-glucose
mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C
1.2
D-glucose
mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C
1.2
D-glucose
mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C
1.3
D-glucose
mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C
1.5
D-glucose
mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C
1.5
D-glucose
mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C
1.5
D-glucose
mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C
1.8
D-glucose
mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C
1.9
D-glucose
mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C
1.9
D-glucose
mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C
2.1
D-glucose
mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C
2.1
D-glucose
mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C
2.2
D-glucose
mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C
2.3
D-glucose
mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C
2.3
D-glucose
mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C
2.5
D-glucose
mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C
3
D-glucose
mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
3.2
D-glucose
wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C
3.5
D-glucose
-
in 50 mM MES buffer (pH 6.0), at 60°C
4.6
D-glucose
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
5.5
D-glucose
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
36.8
D-glucose
mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C
0.14
phosphate
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
210
alpha,alpha-trehalose
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
51.4
alpha-D-arabinose
-
in 50 mM MES buffer (pH 6.0), at 60°C
54
alpha-D-galactose
-
in 50 mM MES buffer (pH 6.0), at 60°C
64.3
alpha-D-xylose
-
in 50 mM MES buffer (pH 6.0), at 60°C
68.1
alpha-L-fucose
-
in 50 mM MES buffer (pH 6.0), at 60°C
1.2
beta-D-galactose 1-phosphate
-
wild type enzyme, at pH 6.0 and 60°C
1.6
beta-D-galactose 1-phosphate
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
7.4
beta-D-glucose 1-phosphate
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
68
beta-D-glucose 1-phosphate
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
114
beta-D-glucose 1-phosphate
-
wild type enzyme, at pH 6.0 and 60°C
0.5
D-galactose
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
14
D-galactose
mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
17
D-galactose
mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C
21.1
D-galactose
mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C
22.6
D-galactose
mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C
23
D-galactose
mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C
23.3
D-galactose
mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
23.6
D-galactose
mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
24
D-galactose
mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C
26.2
D-galactose
mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C
27.7
D-galactose
mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C
31.9
D-galactose
mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C
32.4
D-galactose
mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C
32.9
D-galactose
mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C
32.9
D-galactose
wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C
33.3
D-galactose
mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C
35.5
D-galactose
mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C
38.3
D-galactose
mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C
38.9
D-galactose
mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C
42.2
D-galactose
mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C
54.3
D-galactose
mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C
66.5
D-galactose
mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C
4.2
D-glucose
-
mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C
7
D-glucose
mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
16.1
D-glucose
mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
18.6
D-glucose
mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C
21.7
D-glucose
mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C
25
D-glucose
mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C
26.3
D-glucose
mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C
27.4
D-glucose
mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C
30.6
D-glucose
mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C
31.4
D-glucose
mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C
32.1
D-glucose
mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C
32.7
D-glucose
mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C
33.9
D-glucose
wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C
36.1
D-glucose
mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C
41.2
D-glucose
mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C
42
D-glucose
mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C
42.7
D-glucose
mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C
43.3
D-glucose
mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C
43.5
D-glucose
mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C
46.9
D-glucose
mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C
51.2
D-glucose
mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C
53.9
D-glucose
mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
59
D-glucose
-
in 50 mM MES buffer (pH 6.0), at 60°C
60
D-glucose
Salisediminibacterium selenitireducens
recombinant enzyme, at pH 6.5 and 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.793
alpha-D-arabinose
-
in 50 mM MES buffer (pH 6.0), at 60°C
0.315
alpha-D-galactose
-
in 50 mM MES buffer (pH 6.0), at 60°C
3.17
alpha-D-xylose
-
in 50 mM MES buffer (pH 6.0), at 60°C
0.371
alpha-L-fucose
-
in 50 mM MES buffer (pH 6.0), at 60°C
0.149
D-galactose
mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C
0.336
D-galactose
wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C
0.337
D-galactose
mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C
0.368
D-galactose
mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
0.388
D-galactose
mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C
0.413
D-galactose
mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C
0.444
D-galactose
mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C
0.488
D-galactose
mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C
0.5
D-galactose
mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C
0.509
D-galactose
mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C
0.545
D-galactose
mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C