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Information on EC 2.4.1.5 - dextransucrase and Organism(s) Streptococcus mutans
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IUBMB Comments
The glucansucrases transfer a D-glucosyl residue from sucrose to a glucan chain. They are classified based on the linkage by which they attach the transferred residue. In some cases, in which the enzyme forms more than one linkage type, classification relies on the relative proportion of the linkages that are generated. This enzyme extends the glucan chain by an α(1→6) linkage.
The taxonomic range for the selected organisms is: Streptococcus mutans
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
- 2.4.1.5
- streptococcus
- mutans
-
glucosylation
- glucans
- leuconostoc
-
dental
- mesenteroides
- caries
- udp-glucose
- oligosaccharide
-
cariogenic
- sobrinus
- glucoside
-
water-insoluble
- difficile
- plaque
- maltose
- glycosyltransferases
-
serotype
- saliva
- tooth
- sanguis
- gordonii
- fructosyltransferase
- udp-glc
-
monoglucosylated
- dextranase
- tcda
- glucansucrase
- pellicle
- salivarius
- gtases
-
galactosyltransferase
-
saliva-coated
- calnexin
- viscosus
-
lipid-linked
- industry
- food industry
- aureobasidium
- oralis
- pharmacology
-
pseudomembranous
- medicine
- 3-o-glucoside
- levansucrase
- biotechnology
-
alpha-1,6
- weissella
- man9glcnac2
-
transglucosylation
- synthesis
- glc3man9glcnac2
- nutrition
- isomaltose
- sordellii
-
holotoxins
Reaction Schemes
showSynonyms
glucosyltransferase, dextransucrase, b-512f dextransucrase, dsase, dsr-s, b-512fmc dextransucrase, gtf-dsm, dsrbcb4, dsrwc, dsr-f, more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CEP
-
-
-
-
dextran-sucrase
-
-
-
-
DS
-
-
-
-
DSRS
-
-
-
-
glucosyltransferase, sucrose-1,6-alpha-glucan
-
-
-
-
SGE
-
-
-
-
sucrose 6-glucosyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
sucrose:(1->6)-alpha-D-glucan 6-alpha-D-glucosyltransferase
The glucansucrases transfer a D-glucosyl residue from sucrose to a glucan chain. They are classified based on the linkage by which they attach the transferred residue. In some cases, in which the enzyme forms more than one linkage type, classification relies on the relative proportion of the linkages that are generated. This enzyme extends the glucan chain by an alpha(1->6) linkage.
CAS REGISTRY NUMBER
COMMENTARY
9032-14-8
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
sucrose + dextran
?
-
Substrates: dextransucrase exhibits both hydrolytic and transferase activities. It catalyses the transfer of glucosyl residues from sucrose to dextran to form complex polysaccharides
Products: -
Products: -
?
sucrose + [(1->6)-alpha-D-glucosyl]n
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
Substrates: -
Products: -
Products: -
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
Substrates: -
Products: -
Products: -
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
Substrates: participates in glucan synthesis
Products: -
Products: -
?
additional information
?
-
-
Substrates: purified dextransucrase possesses an invertase-like activity
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme shows hydrolytic and glucosyl transferase activities with sucrose
Products: -
Products: -
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
Substrates: participates in glucan synthesis
Products: -
Products: -
?
sucrose + [(1->6)-alpha-D-glucosyl]n
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
Substrates: -
Products: -
Products: -
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
chitosan
-
various sizes of chitosans (CTSN, CTSN-P, CTSN-B, and CTSN-S) prepared as low molecular weight chitosan show significant inhibitory effect on enzyme DSase activity, antimicrobial activity of CTSNs toward Streptococcus mutans, overview
dextran derivatives
-
in which 5-50% of the D-glucose units are oxidized, acts as potent and specific inhibitor. 10%-oxidized derivatives of dextran fraction ranging in MW from 10000 Da to 2000000 Da
-
gallic acid
-
80-90% inhibition at 4-5 mM, mixed-type inhibition in vitro
NaCl
-
only the hydrolytic activity of the dextransuccrase is influenced by sodium ions, whereas there is no effect on glucosyltransferase activity. 0.5 mM, 65% inhibition of hydrolytic activity, 20 mM, 90% inhibition of hydrolytic activity. The enzyme shows biphasic effects in response to Na+ ions. The enzyme activity is restored by 86% after dialysis compared to the control enzyme preparation. No effect on in presence of other monovalent cations (Rb+, Cs+, and K+). The effects of sodium ions on dextransuccrase activity are specific, thus it can be useful to block its catalytic activity, and reducing the cariogenic potential of Streptococcus mutans
additional information
-
D-glucose has no effect on enzyme ativity; water-soluble extract of Curcuma sp., strong enzyme inhibition
-
additional information
-
aqueous plant extracts from wood sticks of Acacia arabica, Azadirachta indica, Pongamia pinnata, and Salvadora persica are inhibitory. 5 mg Phenolic content in aqueous extracts of chewing stem sticks produce 35-40% inhibition of the enzyme activity, best inhibition at 7-7.5 for the phenolic compounds, and at pH 5.0-6.0 for plant extracts
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
water-soluble extract of Artemisia annua , 2.5fold enzyme activation
-
additional information
-
water-soluble extract of Artemisia sajaval , 3fold enzyme activation
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.8
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 7
-
pH 4.0: about 40% of maximal activity, pH 7.0: about 60% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksGENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
physiological function
-
Streptococcus mutans causes dental caries in humans using sucrose for its growth
metabolism
-
the enzyme is responsible for sucrose metabolism exhibiting both hydrolysis and glucosyl transferase activities
metabolism
-
key enzyme in Streptococcus mutans for the metabolism of sucrose which helps in the adherence and accumulation of bacteria on tooth surface leading to the formation of dental caries
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
-
the effects of sodium ions on dextran succrase activity are specific, thus it can be useful to block its catalytic activity, and reducing the cariogenic potential of Streptococcus mutans
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ono, K.; Nuessle, D.W.; Smith, E.E.
Oxidized saccharides as inhibitors of alpha-glucan synthesis by Streptococcus mutans glucosyltransferase
Carbohydr. Res.
88
119-134
1981
Streptococcus mutans
Takashio, M.; Okami, Y.
Effect of ribocitrin on glucan synthesizing enzymes of Streptococcus mutans E49
Agric. Biol. Chem.
47
2161-2171
1983
Streptococcus mutans, Streptococcus mutans E49
-
Russell, R.R.B.
Purification of Streptococcus mutans glucosyltransferase by polyethylene glycol precipitation
FEMS Microbiol. Lett.
6
197-199
1979
Streptococcus mutans
-
Fukui, K.; Fukui, Y.; Moriyama, T.
Purification and properties of dextransucrase and invertase from Streptococcus mutans
J. Bacteriol.
118
796-804
1974
Streptococcus mutans, Streptococcus mutans HS6
Chludzinski, A.M.; Germaine, G.R.; Schachtele, C.F.
Purification and properties of dextransucrase from Streptococcus mutans
J. Bacteriol.
118
1-7
1974
Streptococcus mutans, Streptococcus mutans 6715
Lee, C.G.; Park, J.K.
Comparison of inhibitory activity of bioactive molecules on the dextransucrase from Streptococcus mutans
Appl. Microbiol. Biotechnol.
99
7495-7503
2015
Streptococcus mutans, Streptococcus mutans ATCC 700610 / UA159
Goyal, D.; Sharma, S.; Mahmood, A.
Inhibition of dextransucrase activity in Streptococcus mutans by plant phenolics
Indian J. Biochem. Biophys.
50
48-53
2013
Streptococcus mutans, Streptococcus mutans MTCC 890
EXTERNAL LINKS (specific for EC-Number 2.4.1.5)
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