Information on EC 2.4.1.44 - lipopolysaccharide 3-alpha-galactosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.44
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RECOMMENDED NAME
GeneOntology No.
lipopolysaccharide 3-alpha-galactosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + lipopolysaccharide = UDP + 3-alpha-D-galactosyl-[lipopolysaccharide glucose]
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Lipid A-core biosynthesis (E. coli K-12)
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lipid A biosynthesis
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Lipopolysaccharide biosynthesis
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-galactose:lipopolysaccharide 3-alpha-D-galactosyltransferase
Transfers alpha-D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide [cf. EC 2.4.1.56 (lipopolysaccharide N-acetylglucosaminyltransferase), EC 2.4.1.58 (lipopolysaccharide glucosyltransferase I) and EC 2.4.1.73 (lipopolysaccharide glucosyltransferase II)].
CAS REGISTRY NUMBER
COMMENTARY hide
9073-98-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
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no activity in absence of divalent cations, 20% of the activation with MgCl2
MgCl2
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no activity in absence of divalent cations, highest activity with MgCl2
MnCl2
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no activity in absence of divalent cations, 20% of the activation with MgCl2
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Nonsubstrate lipopolysaccharide
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competitive, Salmonella typhimurium SL1032, TV119, LT2 lipopolysaccharides; effect on several strains, overview
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Phospholipid
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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several bacterial strains, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Neisseria meningitidis;
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
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4°C, several days, inactivation
489211
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
one cycle of freezing and thawing causes marked loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, pH 6.8, 0.02-0.04 mg/ml protein, stable for at least 1 month
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