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Information on EC 2.4.1.41 - polypeptide N-acetylgalactosaminyltransferase and Organism(s) Homo sapiens and UniProt Accession Q9HCQ5

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.41 polypeptide N-acetylgalactosaminyltransferase
IUBMB Comments
Requires both Mn2+ and Ca2+. The glycosyl residue is transferred to threonine or serine hydroxy groups on the polypeptide core of submaxillary mucin, kappa-casein, apofetuin and some other acceptors of high molecular mass.
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Select one or more organisms in this record:
This record set is specific for:
Homo sapiens
UNIPROT: Q9HCQ5
Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
galnt3, n-acetylgalactosaminyltransferase, galnac-t3, galnac-t, galnac-transferase, galnt14, galnac transferase, galnac-t2, galnac-t1, ppgalnac-t2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(UDP)-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase
277532, 277535, 277539, 284672
-
acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-glycoprotein
-
-
-
-
GalNAc transferase
277532, 277535, 277539, 284672
-
GalNAc-T
GalNAc-T1
GalNAc-T11
GalNAc-T12
247
isoform
GalNAc-T14
GalNAc-T16
247
isoform
GalNAc-T2
GalNAc-T3
GalNAc-T4
GalNAc-T5
247
isoform
GalNAc-T6
GalNAc-T7
247
isoform
GalNAc-transferase
GalNAc-transferase T1
284672
-
GALNT12
277540
-
GALNT14
247
-
GALNT3
247
-
glycopeptide-preferring polypeptide GalNAc transferase 10
247
-
glycoprotein acetylgalactosaminyltransferase
-
-
-
-
N-acetylgalactosaminyltransferase
247
-
polypeptide GalNAc transferase 2
247
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polypeptide GalNAc-transferase
247
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polypeptide GalNAc-transferase 2
247
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polypeptide GalNAc-transferase-2
247
-
polypeptide N-acetylgalactosaminyl transferase-3
277533
-
polypeptide N-acetylgalactosaminyltransferase
polypeptide N-acetylgalactosaminyltransferase 12
277540
-
polypeptide N-acetylgalactosaminyltransferase 14
polypeptide N-acetylgalactosaminyltransferase 2
247
-
polypeptide N-acetylgalactosaminyltransferase 3
247
-
polypeptide N-acetylgalactosaminyltransferase 6
247
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polypeptide-N-acetylgalactosamine transferase
-
-
-
-
polyppetide GalNAc transferase
247
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pp-GalNAc-T13
277541
-
pp-GalNAc-T15
277527
-
pp-GalNAc-T2
277532
-
ppGalNAc T10
247
-
ppGalNAc T13
247
isoform
ppGalNAc T16
247
isoform
ppGalNAc T2
ppGalNAc T3
247
isoform
ppGalNAc T5
247
isoform
ppGalNAc T6
247
isoform
ppGalNAc-T
ppGalNAc-T12
277540
-
ppGalNAc-T2
247
-
ppGalNAc-T6
247
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ppGalNAcT
277532
-
ppGalNAcT or hT
247
-
ppGaNTase
-
-
-
-
ppGaNTase-T11
277539
-
ppGaNTase-T12
277540
-
ppGaNTase-T3
277533
-
ppGaNTase-T6
277538
-
protein-UDP acetylgalactosaminyltransferase
-
-
-
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UDP GalNAc:polypeptide N-acetylgalactosaminyltransferase
277532, 277535, 277539, 284672
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UDP-acetylgalactosamine-glycoprotein acetylgalactosaminyltransferase
-
-
-
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UDP-acetylgalactosamine:peptide-N-galactosaminyltransferase
-
-
-
-
UDP-GalNAc polypeptide:GalNAc transferase-T2
277532
-
UDP-GalNAc polypeptides:N-acetyl-alpha-galactosaminyltransferase
277532
-
UDP-GalNAc transferase
277527, 277532
-
UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase
UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase-2
247
-
UDP-GalNAc:polypeptide N-acetylgalactosaminyl transferase
-
-
-
-
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 18
297794
member of Y subfamily (together with UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 8, 9 and 17)
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3
247
-
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1
247
-
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13
247
-
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13V1
247
splice variant 1 of UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13, a 198 bp intron between exons 10 and 11 is not spliced out resulting in alteration of the lectin domain
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 16
247
-
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 17
247
-
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 18
247
-
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 19
247
-
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 20
247
-
UDP-N-acetyl-alpha-D-galactosaminyltransferase
277533
-
UDP-N-acetyl-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase-6
247
-
UDP-N-acetylgalactosamine polypeptide: N-acetylgalactosaminyl-transferase
277532
-
UDP-N-acetylgalactosamine-glycoprotein N-acetylgalactosaminyltransferase
-
-
-
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UDP-N-acetylgalactosamine-protein N-acetylgalactosaminyltransferase
-
-
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UDP-N-acetylgalactosamine:kappa-casein polypeptide N-acetylgalactosaminyltransferase
-
-
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UDP-N-acetylgalactosamine:polypeptide N-acetyl-alpha-galactosaminyltransferase
277532
-
UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferase
UDP-N-acetylgalactosamine:protein N-acetylgalactosaminyl transferase
-
-
-
-
UDP-N-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase
277532, 277533, 277535, 277538, 284672
-
uridine diphosphate-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3
247
-
uridine diphosphoacetylgalactosamine-glycoprotein acetylgalactosaminyltransferase
-
-
-
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additional information
247
the enzyme belongs to the large family of UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferases, ppGalNAc Ts
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyl-transferase
Requires both Mn2+ and Ca2+. The glycosyl residue is transferred to threonine or serine hydroxy groups on the polypeptide core of submaxillary mucin, kappa-casein, apofetuin and some other acceptors of high molecular mass.
CAS REGISTRY NUMBER
COMMENTARY hide
9075-15-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
show the reaction diagram
the enzyme is involved in the normal development of the brain through O-glycosylation of proteins in the neurons
-
-
?
5 UDP-N-acetyl-D-galactosamine + DSTTPAPTTK
5 UDP + N-acetyl-D-galactosaminylated DSTTPAPTTK
show the reaction diagram
erythropoietin T + UDP-Gal
erythropoietin T-nGalNAc + UDP
show the reaction diagram
hCG-beta + UDP-Gal
hCG-beta-nGalNAc + UDP
show the reaction diagram
-
-
-
?
IgA hinge + UDP-Gal
IgA hinge-nGal + UDP
show the reaction diagram
-
-
-
?
IgA hinge + UDP-GalNAc
IgA hinge-nGalNAc + UDP
show the reaction diagram
IgA hinge-4GalNAc + UDP-GalNAc
IgA hinge-nGalNAc + UDP
show the reaction diagram
-
-
-
?
MUC1 + UDP-GalNAc
MUC1-nGalNAc + UDP
show the reaction diagram
MUC1-2GalNAc + UDP-GalNAc
MUC1-nGalNAc + UDP
show the reaction diagram
-
-
-
?
MUC1a' + UDP-GalNAc
MUC1a'-nGalNAc + UDP
show the reaction diagram
-
-
-
?
MUC1b' + UDP-GalNAc
MUC1b'-nGalNAc + UDP
show the reaction diagram
-
-
-
?
MUC2 + UDP-GalNAc
MUC2-nGalNAc + UDP
show the reaction diagram
-
-
-
?
MUC2-6GalNAc + UDP-GalNAc
MUC2-nGalNAc + UDP
show the reaction diagram
-
-
-
?
MUC5AC + UDP-GalNAc
GalNAc-Thr9-MUC5AC + UDP
show the reaction diagram
-
hT2CD, hT2: glycosylation site selection driven by and substrate binding through its catalytic domain, hT10: not catalysed by hT10 because hT10 does not recognize naked peptides
preferred site for glycosylation by hT2 and hT2CD compared to Thr-3, Thr-10 and Thr-13
-
?
MUC5AC glycopeptides + UDP-GalNAc
GalNAc-MUC5AC + UDP
show the reaction diagram
-
hT2: site preference of glycosylation depends on location of pre-existing GalNAc and is mediated by its lectin domain which directs glycosylation 10 residues N- or C-terminal to an extant GalNAc residue and aids site selection only when potential site is N-terminal to an extant GalNAc residue not C-terminal, Thr-9 is no preferred site of glycosylation, hT10: must recognize existing GalNAc residues via its catalytic domain which directs selection to the glycosylation site immediately N-terminal to the present GalNAc,
-
-
?
MUC5AC-13 + UDP-GalNAc
GalNAc-Thr12-MUC5AC-13 + UDP
show the reaction diagram
-
hT10, hT10CD, hT10CD-hT2LD
-
-
?
MUC5AC-13 + UDP-GalNAc
GalNAc-Thr3-MUC5AC-13 + UDP
show the reaction diagram
-
hT2: substrate binding through its catalytic domain, hT10: transfer of only single GalNAc residues
predominant product of hT2 and hT10
-
?
MUC5AC-13 + UDP-GalNAc
GalNAc-Thr9-MUC5AC-13 + UDP
show the reaction diagram
-
hT2, hT2CD: no initial burst phase during catalysis
not preferred by hT2, preferred by hT2CD
-
?
MUC5AC-3 + UDP-GalNAc
GalNAc-Thr13-MUC5AC-3 + UDP
show the reaction diagram
-
hT2: no initial burst phase during catalysis, substrate binding through its catalytic domain, hT10: transfer of only single GalNAc residues
hT2: preferred site for glycosylation, mediated by enhanced product release through lectin domain, hT10: predominant product
-
?
MUC5AC-3 + UDP-GalNAc
GalNAc-Thr2-MUC5AC + UDP
show the reaction diagram
-
hT10CD, hT10CD-hT2LD, hT10: catalysis faster than for MUCAC-9
-
-
?
MUC5AC-3 + UDP-GalNAc
GalNAc-Thr9-MUC5AC-3 + UDP
show the reaction diagram
-
hT2, hT2CD
not preferred by hT2, hT2CD: preferred site for glycosylation, lack of lectin domain in hT2CD results in rate-limiting product release
-
?
MUC5AC-3,13 + UDP-GalNAc
GalNAc-Ser-5-MUC5AC-3,13 + UDP
show the reaction diagram
-
hT2, not catalysed by hT2CD and hT2CD-hT10LD
preferred site by hT2
-
?
MUC5AC-3,13 + UDP-GalNAc
GalNAc-Thr2,12-MUC5AC-3,13 + UDP
show the reaction diagram
-
hT10, hT10CD, hT10CD-hT2LD
-
-
?
MUC5AC-9 + UDP-GalNAc
GalNAc-Thr2-MUC5AC-9 + UDP
show the reaction diagram
-
not catalysed by hT10CD and hT10CD-hT2LD, hT10: catalysis slower than for MUC5AC-3
preferred site of glycosylation by hT10
-
?
MUC7 + UDP-GalNAc
MUC7-nGalNAc + UDP
show the reaction diagram
-
-
-
?
OSM fragment + UDP-Gal
OSM fragment-nGalNAc + UDP
show the reaction diagram
-
-
-
?
UDP-galactose + Muc1 peptide
UDP + galactosyl-Muc1 peptide
show the reaction diagram
UDP-galactose + Muc2 peptide
UDP + galactosyl-Muc2 peptide
show the reaction diagram
UDP-galactose + PTTTPITTTTK
UDP + galactosyl-PTTTPITTTTK
show the reaction diagram
UDP-GalNAc + EA2
GalNAc-EA2 + UDP
show the reaction diagram
ternary complex of ppGalNAcT-2 in crystal structure PDB: 2FFU, UDP-GalNAc binding results in the closed confirmation of loop B which stabilises loop A by binding of loop A (N102) to loop B (R362), EA2 peptide binds in an extended confirmation
binary complex of UDP and ppGalNAcT-2 in crystal structure PDB: 2FFV
-
?
UDP-GalNAc + GAGA(X)3T(X)3AGAGK
UDP + GAGA(X)3(GalNAc)T(X)3AGAGK
show the reaction diagram
-
-
-
?
UDP-GalNAc + GAGA(X)5T(X)5AGAGK
UDP + GAGA(X)5(GalNAc)T(X)5AGAGK
show the reaction diagram
very high correlation between peptide preferences of ppGalNAc T2 and fly orthologue PGANT2 with r(square) = 0.92
-
-
?
UDP-GalNAc + GAGA(X)nT(X)nAGAGK
GAGA(X)n(GalNAc)T(X)nAGAGK + UDP
show the reaction diagram
library of unmodified peptides, with randomized residue at position X and n=3 or n=5, random peptide preferences are not significantly altered by increased peptide substrate length n=5 compared to n=3 or the proximity of flanking Gly and Ala residues to the site of glycosylation, major peptide substrate preference determinants are the 2 to 3 residues flanking the site of glycosylation
-
-
?
UDP-GalNAc + HGVTSAPDTRPAPGSTAPPA
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-GalNAc + PTTTPISTTTMVTPTPTPTC
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-GalNAc + VLTTTATTPTA
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glycosylated GTTPSPVPTTSTTSAP)
UDP + ?
show the reaction diagram
-
natural-type (alpha-GalNAc-O-Thr) and unnatural-type (beta-GalNAc-O-Thr, alpha-Fuc-O-Thr and beta-GlcNAc-O-Thr) glycosylated
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Ac-CIRIQRGPGRAFVTIGKIGNMR
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Ac-QATEYEYLDYDFLPETEPPEM
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + AHGVTSAPDTR
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GAGAEAPTPAPAGAGK
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTAKPTTLKPTE
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + GT[GalNAc]TPSPVPTTSTTSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + GT[GalNAc]TPSPVPTTST[GalNAc]TSAP
show the reaction diagram
-
-
glycosylation at Thr-3 and Thr-13
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + GT[GalNAc]TP[GalNAc]SPVPTTSTTSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTST[GalNAc]TSAP
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTST[GalNAc]TSAP
UDP + GT[GalNAc]TPSPVPTTST[GalNAc]TSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTST[GalNAc]TSAP
UDP + GT[GalNAc]TP[GalNAc]SPVPTTST[GalNAc]TSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GT[GalNAc]TPSPVPTTSTTSAP
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GT[GalNAc]TPSPVPTTSTTSAP
UDP + GT[GalNAc]TPSPVPTTS[GalNAc]T[GalNAc]TSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GT[GalNAc]TPSPVPTTSTTSAP
UDP + G[GalNAc]T[GalNAc]TPSPVPTTST[GalNAc]TSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GT[GalNAc]TPSPVPTTST[GalNAc]TSAP
UDP + GT[GalNAc]TPSPVPTTS[GalNAc]T[GalNAc]TSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + PDTRPAPGSTAPPAC
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide angiopoietin-like 3
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide angiopoietin-like 3
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide EA2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide EA2
show the reaction diagram
-
sequence PTTDSTTPAPTTK
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide FGF23
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide FGF23
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPIV
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPIV
show the reaction diagram
-
preferred substrate for isoforms ppGalNAc T2, T5, T13, T14, and T16
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPIV-C
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPIV-C
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPV
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPV
show the reaction diagram
-
preferred substrate for isoforms ppGalNAc T3, T5, T6, T13, and T16
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPV-C
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPV-C
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide MUC13
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide MUC13
show the reaction diagram
-
isoform GALNT14 contributes to the glycosylation of peptide MUC13, which is significantly higher in ovarian cancer cells compared with the normal/benign ovary tissues
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + PSTPPTPSPSTPPTPSPS
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + SPTTSTPISSTPQPTS
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + Ac-CIRIQRGPGRAFVTIGKIGNMR
UDP + N-acetyl-D-galactosaminylated Ac-CIRIQRGPGRAFVTIGKIGNMR
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + Ac-PFVTHPGYD
UDP + N-acetyl-D-galactosaminylated Ac-PFVTHPGYD
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + Ac-QATEYEYLDYDFLPETEPPEM
UDP + N-acetyl-D-galactosaminylated Ac-QATEYEYLDYDFLPETEPPEM
show the reaction diagram
UDP-N-acetyl-D-galactosamine + AHGVTSAPDTR
UDP + N-acetyl-D-galactosaminylated AHGVTSAPDTR
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + AHGVVTSAPDTR
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + ANTPSFPTATSPAPPI
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
show the reaction diagram
-
acceptor: apomucin motif encoded by the MUC5AC gene
-
-
?
UDP-N-acetyl-D-galactosamine + CPPTPSATTPAPPSSSAPPETTAA
UDP + N-acetyl-D-galactosaminylated CPPTPSATTPAPPSSSAPPETTAA
show the reaction diagram
UDP-N-acetyl-D-galactosamine + fibronectin
UDP + N-acetyl-D-galactosaminyl-fibronectin
show the reaction diagram
-
fibronectin is a physiological substrate for GalNAc-T3
-
-
?
UDP-N-acetyl-D-galactosamine + GAGAPGPTPGPAGAGK
UDP + GAGAPGP-(N-acetyl-D-galactosaminylT)-PGPAGAGK
show the reaction diagram
-
optimal isozyme ppGalNAc T2 peptide substrate
-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSA
UDP + N-acetyl-D-galactosaminyl-[GTTPSPVPTTSTTSA]
show the reaction diagram
-
i.e. Muc5Ac peptide
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAk
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + HIV-V3 peptide
UDP + N-acetyl-D-galactosaminyl-HIV-V3 peptide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide
UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + immunoglobulin A1
UDP + N-acetyl-D-galactosaminyl-immunoglobulin A1
show the reaction diagram
-
O-linked glycosylation of the human IgA1 hinge region by ppGalNAc-T2, synthetic IgA hinge peptide: ppGalNAc-T2 shows strongest activity and is able to transfer GalNAc to almost all possible glycosylation sites, other ppGalNAc-Ts show extremely weak activities
-
-
?
UDP-N-acetyl-D-galactosamine + osteopontin
UDP + N-acetyl-D-galactosaminyl-osteopontin
show the reaction diagram
-
isozyme ppGalNAc T10 prefers previously glycosylated peptides as substrates, ppGalNAc T10 exhibits a single large preference for Ser/Thr-O-GalNAc at the +1, C-terminal, position relative to the Ser or Thr acceptor site
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PRFQDSSSSKAPPPSLPSPSRL
UDP + N-actetyl-D-galactosaminylated PRFQDSSSSKAPPPSLPSPSRL
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + PRFQDSSSSKAPPPSLPSPSRLPG
UDP + N-acetyl-D-galactosaminylated PRFQDSSSSKAPPPSLPSPSRLPG
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTT
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PTTTPISTTMVTPTPTPTC
UDP + N-actetyl-D-galactosaminylated PTTTPISTTMVTPTPTPTC
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + PTTTPISTTTMVTPTPTPTC
UDP + N-acetyl-D-galactosaminylated PTTTPISTTTMVTPTPTPTC
show the reaction diagram
mucin derived substrate
-
-
?
UDP-N-acetyl-D-galactosamine + PTTTPITTTTTVTPTPTPTGTQT
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + QATEYEYLDYDFLPEC
UDP + N-acetyl-D-galactosaminylated QATEYEYLDYDFLPEC
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + RPAPGSTAPPA
UDP + N-acetyl-D-galactosaminylated RPAPGSTAPPA
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + syndecan-3
UDP + N-acetyl-D-galactosaminyl-syndecan-3
show the reaction diagram
syndecan-3 may be a natural substrate for GalNAc-T13
-
-
?
UDP-N-acetyl-D-galactosamine + TAPPAHGVTSAPDTRPAPGSTAPP
UDP + N-acetyl-D-galactosaminylated TAPPAHGVTSAPDTRPAPGSTAPP
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
show the reaction diagram
Q9HCQ5
the enzyme is involved in the normal development of the brain through O-glycosylation of proteins in the neurons
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide EA2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide EA2
show the reaction diagram
-
sequence PTTDSTTPAPTTK
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide MUC13
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide MUC13
show the reaction diagram
-
isoform GALNT14 contributes to the glycosylation of peptide MUC13, which is significantly higher in ovarian cancer cells compared with the normal/benign ovary tissues
-
-
?
UDP-N-acetyl-D-galactosamine + fibronectin
UDP + N-acetyl-D-galactosaminyl-fibronectin
show the reaction diagram
-
fibronectin is a physiological substrate for GalNAc-T3
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + syndecan-3
UDP + N-acetyl-D-galactosaminyl-syndecan-3
show the reaction diagram
Q8IUC8
syndecan-3 may be a natural substrate for GalNAc-T13
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
not activated by Mg2+ or Ca2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
benzyl N-acetyl-alpha-D-galactosamine
-
-
benzyl-alpha-GalNAc
-
fully GalNAc-substituted MUC1 repeat peptide
-
inhibits activity of rGalNAc-T4 and -T2
-
GalNAc
HIV-V3
-
competitive inhibition of Muc2 glycosylation
-
methyl-alpha-Gal
-
Muc2
-
competitive inhibition of HIV-V3 peptide glycosylation
-
N-acetyl-alpha-D-galactosamine
-
the acetylated enzyme is strongly inhibited by N-acetyl-alpha-D-galactosamine
o-nitrophenyl-alpha-GalNAc
-
o-nitrophenyl-beta-GalNAc
-
p-nitrophenyl-alpha-GalNAc
-
p-nitrophenyl-beta-GalNAc
-
phenyl-alpha-GalNAc
-
UDP-alpha-GalNAc
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
GalNAc-T7: selectively activated by partial GalNAc glycosylation of peptide substrates derived from the tandem repeats of human Muc2 and rat submaxillary gland mucin
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.45 - 0.91
AHGVVTSAPDTR
0.01 - 0.48
CPPTPSATTPAPPSSSAPPETTAA
0.07 - 2.19
DSTTPAPTTK
0.067 - 0.344
GTTPSPVPTTSTTSAP
0.115 - 0.46
GTTPSPVPTTST[GalNAc]TSAP
0.035 - 0.332
GT[GalNAc]TPSPVPTTSTTSAP
0.206
GT[GalNAc]TPSPVPTTST[GalNAc]TSAP
-
pH not specified in the publication, 37°C