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Information on EC 2.4.1.41 - polypeptide N-acetylgalactosaminyltransferase and Organism(s) Drosophila melanogaster and UniProt Accession Q8MVS5

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.41 polypeptide N-acetylgalactosaminyltransferase
IUBMB Comments
Requires both Mn2+ and Ca2+. The glycosyl residue is transferred to threonine or serine hydroxy groups on the polypeptide core of submaxillary mucin, kappa-casein, apofetuin and some other acceptors of high molecular mass.
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This record set is specific for:
Drosophila melanogaster
UNIPROT: Q8MVS5
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Word Map
The taxonomic range for the selected organisms is: Drosophila melanogaster
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
galnt3, n-acetylgalactosaminyltransferase, galnt2, galnac-t3, galnac-t, galnt14, galnac-transferase, galnac transferase, galnac-t2, galnac-t1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase
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acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-glycoprotein
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GalNAc-transferase
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glycoprotein acetylgalactosaminyltransferase
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polypeptide-N-acetylgalactosamine transferase
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ppGalNAc-T
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ppGaNTase
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protein-UDP acetylgalactosaminyltransferase
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UDP-acetylgalactosamine-glycoprotein acetylgalactosaminyltransferase
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UDP-acetylgalactosamine:peptide-N-galactosaminyltransferase
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UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase
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UDP-GalNAc:polypeptide N-acetylgalactosaminyl transferase
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UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase
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-
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UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase
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-
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UDP-N-acetylgalactosamine-glycoprotein N-acetylgalactosaminyltransferase
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-
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UDP-N-acetylgalactosamine-protein N-acetylgalactosaminyltransferase
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UDP-N-acetylgalactosamine:kappa-casein polypeptide N-acetylgalactosaminyltransferase
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UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferase
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UDP-N-acetylgalactosamine:protein N-acetylgalactosaminyl transferase
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uridine diphosphoacetylgalactosamine-glycoprotein acetylgalactosaminyltransferase
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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hexosyl group transfer
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyl-transferase
Requires both Mn2+ and Ca2+. The glycosyl residue is transferred to threonine or serine hydroxy groups on the polypeptide core of submaxillary mucin, kappa-casein, apofetuin and some other acceptors of high molecular mass.
CAS REGISTRY NUMBER
COMMENTARY hide
9075-15-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-GalNAc + polypeptide
GalNAc-alpha-polypeptide + UDP
show the reaction diagram
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major mucin-type O-glycan in embryonic tracheal system along the apical region and luman of the tracheal tubes O-glycan, detectable by lectins Jacalin, soybean agglutinin and Vicia villosa agglutinin
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?
UDP-N-acetyl-alpha-D-galactosamine + EA2 peptide
?
show the reaction diagram
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-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
show the reaction diagram
acceptor: MUC5AC-13 glycopeptide
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-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
show the reaction diagram
UDP-GalNAc + GAGA(X)3T(X)3AGAGK
GAGA(X)3(GalNAc)T(X)3AGAGK + UDP
show the reaction diagram
-
-
-
?
UDP-GalNAc + GAGA(X)5T(X)5AGAGK
GAGA(X)5(GalNAc)T(X)5AGAGK + UDP
show the reaction diagram
UDP-GalNAc + GAGA(X)nT(X)nAGAGK
GAGA(X)n(GalNAc)T(X)nAGAGK + UDP
show the reaction diagram
library of unmodified peptides, with randomized residue at position X and n=3 or n=5
-
-
?
UDP-N-acetyl-D-galactosamine + GKPRPYSPRPTSHPRPIRV
UDP + ?
show the reaction diagram
i.e. drosocin, low activity, enzyme form PGANT8
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + mucin MUC4AC-13
UDP + ?
show the reaction diagram
enzyme form PGANT2
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3/13
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + mucin MUC5AX-13
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PDTRPAPGSTAPPAC
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
show the reaction diagram
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + PSTPPTPSPSTPPTPSPS
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + SPTTSTPISSTPQPTS
UDP + ?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
show the reaction diagram
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
show the reaction diagram
mucin-type O-linked glycosylation of serine or threonine residues
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-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.595
EA2 peptide
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-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
cloning of the pgant35A gene from embryonic cDNA library
Manually annotated by BRENDA team
dorsal longitudinal tracheal trunks and tracheal transverse connectives at embryonic stages 15-17, loss of PGANT35A activity leads to tracheal defects at embryonic stages 15-17, altered intracellular trafficking, reduction in apical and luminal epithelial marker components, alterations in cell shape, polarity and zona adherens formation, mislocation and disfunction of septate junctions, disruption of paracellular diffusion barrier formation
Manually annotated by BRENDA team
loss of PGANT35A activity does not alter morphology or staining patterns of epithelial marker components
Manually annotated by BRENDA team
loss of PGANT35A activity does not alter morphology or staining patterns of epithelial marker components
Manually annotated by BRENDA team
loss of PGANT35A activity leads to diminished O-glycosylation in epithelial cells of tracheal tube as well as reduced amount of GalNAc alpha-S/T glycans at the apical cell surface
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
type II membrane protein containing a 23-amino acid transmembrane domain and an 8-amino acid cytoplasmic tail
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GLT35_DROME
632
1
71828
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
78000
x * 78000, recombinant wild type and SF32 mutant enzyme, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 78000, recombinant wild type and SF32 mutant enzyme, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L195stop
3775 mutant, more than half of the putative catalytic region is eliminated, early pupal lethality
pgant35A-3775
transgenic fly: heterozygous with pgant35A-SF32 or pgant35A-HG8 allele, germ line clone: heterozygous with pgant35A-HG8 or pgant35A-SF32 allele, nonsense mutation results in translational stop and loss of enzymatic activity
pgant35A-HG8
transgenic fly: heterozygous with pgant35A-SF32 or pgant35A-3775 phenotype, germ line clone: homozygous or with pgant35A-3775 allele , nonsense mutation results in translational stop and loss of enzymatic activity
pgant35A-SF32
transgenic fly: heterozygous with pgant35A-3775 or pgant35A-HG8 phenotype, germ line clone: homozygous or with pgant35A-3775 allele, missense mutation: Arg to Trp, results in diminished activity
Q89stop
HG8 mutant, truncated protein within the putative stem region, early pupal lethality
R227W
SF32 mutant with dramatically reduced activity, early pupal lethality
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme, expressed in COS7 cells
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning, sequencing and expression of wild type and SF32 mutant recombinant pgant35A gene in COS7 cells
PGANT35A in pUAST plasmid for trachea-specific, Gal4-iduceable expression in transgenic flies under control of breathless promoter
expression in COS7 cells as truncated version from pIMKF4 SV40
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ten Hagen, K.G.; Tran, D.T.
A UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is essential for viability in Drosophila melanogaster
J. Biol. Chem.
277
22616-22622
2002
Drosophila melanogaster (Q8MVS5), Drosophila melanogaster
Manually annotated by BRENDA team
Ten Hagen, K.G.; Tran, D.T.; Gerken, T.A.; Stein, D.S.; Zhang, Z.
Functional characterization and expression analysis of members of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase family from Drosophila melanogaster
J. Biol. Chem.
278
35039-35048
2003
Drosophila melanogaster, Drosophila melanogaster (Q6WV16), Drosophila melanogaster (Q6WV17), Drosophila melanogaster (Q6WV19), Drosophila melanogaster (Q6WV20), Drosophila melanogaster (Q8IA42), Drosophila melanogaster (Q8MV48), Drosophila melanogaster (Q9Y117)
Manually annotated by BRENDA team
Gerken, T.A.; Hagen, K.G.; Jamison, O.
Conservation of peptide acceptor preferences between Drosophila and mammalian polypeptide-GalNAc transferase orthologue pairs
Glycobiology
18
861-870
2008
Bos taurus (Q07537), Homo sapiens (Q10471), Drosophila melanogaster (Q6WV17), Drosophila melanogaster (Q6WV19)
Manually annotated by BRENDA team
Tian, E.; ten Hagen, K.G.
A UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is required for epithelial tube formation
J. Biol. Chem.
282
606-614
2007
Drosophila melanogaster (Q8MVS5)
Manually annotated by BRENDA team