Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.4.1.40 - glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase

for references in articles please use BRENDA:EC2.4.1.40
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
Acts on blood group substance, and can use a number of 2-fucosyl-galactosides as acceptors.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
UNIPROT: P16422
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
a transferase, a-transferase, glycosyltransferases a, alpha-3-n-acetylgalactosaminyltransferase, blood group a glycosyltransferase, histo-blood group a transferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A-transferase
-
-
-
-
alpha-3-N-acetylgalactosaminyltransferase
-
-
-
-
blood-group substance A-dependent acetylgalactosaminyltransferase
-
-
-
-
blood-group substance alpha-acetyltransferase
-
-
-
-
fucosylgalactose acetylgalactosaminyltransferase
-
-
-
-
histo-blood group A acetylgalactosaminyltransferase
-
-
-
-
histo-blood group A glycosyltransferase (Fucalpha1-2Galalpha1-3-N-acetylgalactosaminyltransferase)
-
-
-
-
histo-blood group A transferase
-
-
-
-
UDP-GalNAc:Fucalpha1-2Galalpha1-3-N-acetylgalactosaminyltransferase
-
-
-
-
UDP-N-acetyl-D-galactosamine:alpha-L-fucosyl-1,2-D-galactose 3-N-acetyl-D-galactosaminyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose 3-N-acetyl-D-galactosaminyltransferase
Acts on blood group substance, and can use a number of 2-fucosyl-galactosides as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
9067-69-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-galactosamine + alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
UDP + alpha-D-GalNAc-(1->3)-[alpha-Fuc-(1->2)]-beta-D-Gal-(CH2)7CH3
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0099 - 0.045
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
0.0087 - 0.022
UDP-N-acetyl-alpha-D-galactosamine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.6 - 17.5
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
EPCAM_HUMAN
314
0
34932
Swiss-Prot
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
structures of wild-type and mutant D302C. Conserved active site residues Arg188 and Asp302 are critical for catalysis, and disruption of their hydrogen bond network through mutation can dramatically decrease enzymatic activity
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D302A
almost complete loss of activity
D302A/D316A
very low residual activity
D302C
kcat value is 9.5% that of wild-type GTB
D302E
kcat value is 47% that of wild-type GTB
D302E/D316E
almost complete loss of activity
D302L
almost complete loss of activity
D302N
almost complete loss of activity
R188K
almost complete loss of activity
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gagnon, S.; Legg, M.; Polakowski, R.; Letts, J.; Persson, M.; Lin, S.; Zheng, R.; Rempel, B.; Schuman, B.; Haji-Ghassemi, O.; Borisova, S.; Palcic, M.; Evans, S.
Conserved residues Arg188 and Asp302 are critical for active site organization and catalysis in human ABO(H) blood group A and B glycosyltransferases
Glycobiology
28
624-636
2018
Homo sapiens (P16422)
Manually annotated by BRENDA team