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IUBMB Comments The enzyme, characterized from the plant Vigna angularis (azuki beans), participates in the biosynthesis of rhamnogalacturonan I, one of the components of pectin in plant cell wall. It does not require any metal ions, and prefers substrates with a degree of polymerization larger than 9.
2.4.1.375
notch
o-glucosyltransferase
dowling-degos
pofut1
factor-like
o-glucosylation
o-glucose
limb-girdle
suppurativa
psenen
genodermatosis
reticulate
hypoglycosylation
autosomal-dominant
hidradenitis
acantholysis
presenilin
inversa
Reaction Schemes
Transfer of a beta-galactosyl residue in a beta-(1->4) linkage from UDP-alpha-D-galactose to rhamnosyl residues within the rhamnogalacturonan I backbone
Synonyms
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beta-1,4-galactan galactosyltransferase
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POGLUT1
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gene name, ambiguous
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RG-I galactosyltransferase
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rhamnogalacturonan I beta(1->4)galactosyltransferase
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rhamnogalacturonan I galactosyltransferase
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Rumi
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gene name, ambiguous
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Transfer of a beta-galactosyl residue in a beta-(1->4) linkage from UDP-alpha-D-galactose to rhamnosyl residues within the rhamnogalacturonan I backbone
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UDP-alpha-D-galactose:[rhamnogalacturonan I]-alpha-L-rhamnosyl beta-1,4-galactosyltransferase (configuration-inverting)
The enzyme, characterized from the plant Vigna angularis (azuki beans), participates in the biosynthesis of rhamnogalacturonan I, one of the components of pectin in plant cell wall. It does not require any metal ions, and prefers substrates with a degree of polymerization larger than 9.
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UDP-alpha-D-galactose + GR10-PA
UDP + GR10(Gal)-PA
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Substrates: i.e. alpha-D-galacturonosyl-(1->2)-alpha-L-rhamnosyl-(1->4)-alpha-D-galacturonosyl-(1->2)-alpha-L-rhamnosyl-(1->4)-alpha-D-galacturonosyl-(1->2)-alpha-L-rhamnosyl-(1->4)-alpha-D-galacturonosyl-(1->2)-alpha-L-rhamnosyl-(1->4)-alpha-D-galacturonosyl-(1->2)-alpha-L-rhamnosyl-pyridylamino
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UDP-alpha-D-galactose + GR12-PA
UDP + GR12(Gal)-PA
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Substrates: highest activity
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UDP-alpha-D-galactose + GR14-PA
UDP + GR14(Gal)-PA
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Substrates: second highest activity
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UDP-alpha-D-galactose + pectic rhamnogalacturonan I
UDP + 1,4-beta-galactan side chains in pectic rhamnogalacturonan I
UDP-alpha-D-galactose + RG9-PA
UDP + RG9(Gal)-PA
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Substrates: -
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UDP-alpha-D-galactose + rhamnogalacturonan I
UDP + 1,4-beta-galactan side chains in rhamnogalacturonan I
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Substrates: -
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additional information
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UDP-alpha-D-galactose + pectic rhamnogalacturonan I
UDP + 1,4-beta-galactan side chains in pectic rhamnogalacturonan I
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Substrates: -
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UDP-alpha-D-galactose + pectic rhamnogalacturonan I
UDP + 1,4-beta-galactan side chains in pectic rhamnogalacturonan I
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Substrates: -
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UDP-alpha-D-galactose + pectic rhamnogalacturonan I
UDP + 1,4-beta-galactan side chains in pectic rhamnogalacturonan I
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Substrates: pectic rhamnogalacturonan I with short galactan chains of 21 or 1200 kDa
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additional information
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Substrates: pectic rhamnogalacturonan I with short galactan chains of 2 kDa or 1.2 kDa do not serve as acceptor substrates
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additional information
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Substrates: no activity with RG3-PA, GR84-PA RG5-PA, GR6-PA, RG7-PA, and GR8-PA
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UDP-alpha-D-galactose + pectic rhamnogalacturonan I
UDP + 1,4-beta-galactan side chains in pectic rhamnogalacturonan I
UDP-alpha-D-galactose + rhamnogalacturonan I
UDP + 1,4-beta-galactan side chains in rhamnogalacturonan I
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Substrates: -
?
UDP-alpha-D-galactose + pectic rhamnogalacturonan I
UDP + 1,4-beta-galactan side chains in pectic rhamnogalacturonan I
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Substrates: -
?
UDP-alpha-D-galactose + pectic rhamnogalacturonan I
UDP + 1,4-beta-galactan side chains in pectic rhamnogalacturonan I
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Substrates: -
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Mg2+
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10 mM used in assay conditions
Mg2+
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maximum activity at 5 mM
Mn2+
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10 mM used in assay conditions
Mn2+
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maximum activity at 7.5 mM
Mn2+
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10 mM used in assay conditions
Mn2+
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highest activity in the presence of 3 mM Mn2+
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Cu2+
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complete inhibition at 3 mM
Fe2+
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complete inhibition at 3 mM
NaCl
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complete inhibition at 300 mM
Zn2+
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complete inhibition at 3 mM
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benzalkonium chloride
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3 mM benzalkonium chloride increases the enzyme activity 14fold
Cetylpyridinium chloride
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3 mM cetylpyridinium chloride increases the enzyme activity about 25fold
cetyltrimethylammonium bromide
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3 mM cetyltrimethylammonium bromide increases the enzyme activity 29fold
polyallylamine
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0.01 mM polyallylamine increases the enzyme activity 40fold
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Breast Neoplasms
Protein O-glucosyltransferase 1 overexpression downregulates p16 in BT474 human breast cancer cells.
Cholangitis
POGLUT1, the putative effector gene driven by rs2293370 in primary biliary cholangitis susceptibility locus chromosome 3q13.33.
Dysostoses
Diseases related to Notch glycosylation.
Endometrial Neoplasms
MicroRNA-134 suppresses endometrial cancer stem cells by targeting POGLUT1 and Notch pathway proteins.
Liver Cirrhosis, Biliary
POGLUT1, the putative effector gene driven by rs2293370 in primary biliary cholangitis susceptibility locus chromosome 3q13.33.
Lymphoma
Protein O-glucosyltransferase 1 overexpression downregulates p16 in BT474 human breast cancer cells.
Muscle Weakness
POGLUT1 biallelic mutations cause myopathy with reduced satellite cells, ?-dystroglycan hypoglycosylation and a distinctive radiological pattern.
Muscular Diseases
POGLUT1 biallelic mutations cause myopathy with reduced satellite cells, ?-dystroglycan hypoglycosylation and a distinctive radiological pattern.
Muscular Dystrophies
A POGLUT1 mutation causes a muscular dystrophy with reduced Notch signaling and satellite cell loss.
Muscular Dystrophies
POGLUT1 biallelic mutations cause myopathy with reduced satellite cells, ?-dystroglycan hypoglycosylation and a distinctive radiological pattern.
Muscular Dystrophies, Limb-Girdle
A POGLUT1 mutation causes a muscular dystrophy with reduced Notch signaling and satellite cell loss.
Muscular Dystrophies, Limb-Girdle
Diseases related to Notch glycosylation.
Muscular Dystrophies, Limb-Girdle
Generation of an induced pluripotent stem cell line (CSCRMi001-A) from a patient with a new type of limb-girdle muscular dystrophy (LGMD) due to a missense mutation in POGLUT1 (Rumi).
Muscular Dystrophies, Limb-Girdle
POGLUT1 biallelic mutations cause myopathy with reduced satellite cells, ?-dystroglycan hypoglycosylation and a distinctive radiological pattern.
Neoplasms
Diseases related to Notch glycosylation.
Neoplasms
MicroRNA-134 suppresses endometrial cancer stem cells by targeting POGLUT1 and Notch pathway proteins.
Neoplasms
Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse Gastrulation through Modification of the Apical Polarity Protein CRUMBS2.
Nephrosis
Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse Gastrulation through Modification of the Apical Polarity Protein CRUMBS2.
Retinal Degeneration
Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse Gastrulation through Modification of the Apical Polarity Protein CRUMBS2.
Retinitis Pigmentosa
Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse Gastrulation through Modification of the Apical Polarity Protein CRUMBS2.
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0.2 - 4
UDP-alpha-D-galactose
0.12
GR10-PA
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enzyme hyperactivated with 3 mM benzalkonium chloride, at pH 7.0 and 30°C
0.78
GR10-PA
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at pH 7.0 and 30°C
0.2
UDP-alpha-D-galactose
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enzyme hyperactivated with 3 mM benzalkonium chloride, at pH 7.0 and 30°C
0.46
UDP-alpha-D-galactose
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at pH 8.0 and 30°C
4
UDP-alpha-D-galactose
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at pH 7.0 and 30°C
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5.6
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pH optimum for transfer of galatosyl residues onto pectic rhamnogalacturonan I with short galactan chains of 21 kDa
6.5
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the enzyme has two pH optima at pH 6.5 and 8.0
7.5
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pH optimum for transfer of galatosyl residues onto pectic rhamnogalacturonan I with short galactan chains of 1.2 MDa
8
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the enzyme has two pH optima at pH 6.5 and 8.0
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30
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cultivar Viking
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brenda
azuki bean, cultivar Erimo-wase
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brenda
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brenda
variant AZY
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brenda
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brenda
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brenda
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brenda
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the enzyme is integrated into the membrane of the Golgi apparatus with its catalytic site facing the lumen
brenda
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brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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400000 - 500000
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gel filtration
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47
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after 5 min at 47 C the enzyme retains 50% of its initial activity. Beyond 60 C, the enzyme is completely inactivated
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approximately 75% of the enzyme activity is lost after treatment with proteinase in the presence of Triton X-100. In addition, the enzyme activity is recovered in the detergent phase after treatment of Golgi vesicles with Triton X-114
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Geshi, N.; Pauly, M.; Ulvskov, P.
Solubilization of galactosyltransferase that synthesizes 1,4-beta-galactan side chains in pectic rhamnogalacturonan I
Physiol. Plant.
114
540-548
2002
Solanum tuberosum
brenda
Geshi, N.; Jorgensen, B.; Ulvskov, P.
Subcellular localization and topology of beta(1-4)galactosyltransferase that elongates beta(1-4)galactan side chains in rhamnogalacturonan I in potato
Planta
218
862-868
2004
Solanum tuberosum
brenda
Matsumoto, N.; Takenaka, Y.; Wachananawat, B.; Kajiura, H.; Imai, T.; Ishimizu, T.
Rhamnogalacturonan I galactosyltransferase Detection of enzyme activity and its hyperactivation
Plant Physiol. Biochem.
142
173-178
2019
Vigna angularis
brenda
Geshi, N.; Jergensen, B.; Scheller, H.; Ulvskov, P.
In vitro biosynthesis of 1,4-beta-galactan attached to rhamnogalacturonan I
Planta
210
622-629
2000
Solanum tuberosum
brenda
Peugnet, I.; Goubet, F.; Bruyant-Vannier, M.; Thoiron, B.; Morvan, C.; Schols, H.; Voragen, A.
Solubilization of rhamnogalacturonan I galactosyltransferases from membranes of a flax cell suspension
Planta
213
435-445
2001
Linum usitatissimum
brenda
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