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IUBMB CommentsThe enzyme has been characterized from the saponin-producing crucifer plant Barbarea vulgaris.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
ugt73c11, ugt73c10,
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UDP-glycosyltransferase
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UDP-glycosyltransferase
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UGT73C10
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UGT73C11
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UDP-alpha-D-glucose + oleanolate = UDP + oleanolate 3-O-beta-D-glucoside
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UDP-alpha-D-glucose:oleanolate 3-O-glucosyltransferase
The enzyme has been characterized from the saponin-producing crucifer plant Barbarea vulgaris.
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UDP-alpha-D-glucose + glycyrrhetinic acid
UDP + glycyrrhetinic acid 3-O-beta-monoglucose
the highest conversion is found at the substrate molar ratio UDP-alpha-D-glucose/glycyrrhetinate of 5:1 with 98% conversion after 6 h
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UDP-alpha-D-glucose + oleanolate
UDP + oleanolate 3-O-beta-D-glucoside
the enzyme specifically glucosylates oleanoate at the C3 position
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additional information
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additional information
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no activity with UDP-glucuronic acid
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additional information
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the enzyme shows weak activity toward quercetin and kaempferol at 0.1 mM concentration, while at 0.01 mM no activity detected
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additional information
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the enzyme shows weak activity toward quercetin and kaempferol at 0.1 mM concentration, while at 0.01 mM no activity detected
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UDP-alpha-D-glucose + oleanolate
UDP + oleanolate 3-O-beta-D-glucoside
the enzyme specifically glucosylates oleanoate at the C3 position
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Mg2+
about 110% activity at 5 mM
additional information
not influenced by Ba2+, Ni2+, Mn2+, and Ca2+
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Co2+
about 68% residual activity at 5 mM
Cu2+
complete inhibition at 5 mM
Fe2+
about 85% residual activity at 5 mM
Zn2+
about 22% residual activity at 5 mM
additional information
not inhibited by EDTA
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0.104
glycyrrhetinic acid
at pH 7.4 and 37°C
0.486
UDP-alpha-D-glucose
at pH 7.4 and 37°C
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0.091
glycyrrhetinic acid
at pH 7.4 and 37°C
0.102
UDP-alpha-D-glucose
at pH 7.4 and 37°C
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0.873
glycyrrhetinic acid
at pH 7.4 and 37°C
0.215
UDP-alpha-D-glucose
at pH 7.4 and 37°C
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6 - 8
more than 60% activity between pH 6.0 and 8.0
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25 - 45
more than 50% activity between 25 and 45°C
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UniProt
brenda
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brenda
Barbarea vulgaris subsp. arcuata
UniProt
brenda
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metabolism
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the enzyme is involved in saponin biosynthesis
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73C10_BARVU
495
0
55486
Swiss-Prot
Secretory Pathway (Reliability: 5)
73C11_BARVU
495
0
55613
Swiss-Prot
Secretory Pathway (Reliability: 5)
73C12_BARVU
495
0
55576
Swiss-Prot
other Location (Reliability: 5)
73C13_BARVU
495
0
55427
Swiss-Prot
Secretory Pathway (Reliability: 5)
UGT1_BARVU
495
0
55599
Swiss-Prot
Secretory Pathway (Reliability: 5)
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Ni-NTA column chromatography
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli XJb(DE3) cells
expressed in Nicotiana benthamiana
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Liu, X.; Zhang, L.; Feng, X.; Lv, B.; Li, C.
Biosynthesis of glycyrrhetinic acid-3-O-monoglucose using glycosyltransferase UGT73C11 from Barbarea vulgaris
Ind. Eng. Chem. Res.
56
14949-14958
2017
Barbarea vulgaris (K4GGT4)
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brenda
Erthmann, P.O.; Agerbirk, N.; Bak, S.
A tandem array of UDP-glycosyltransferases from the UGT73C subfamily glycosylate sapogenins, forming a spectrum of mono- and bisdesmosidic saponins
Plant Mol. Biol.
97
37-55
2018
Barbarea vulgaris
brenda
Augustin, J.M.; Drok, S.; Shinoda, T.; Sanmiya, K.; Nielsen, J.K.; Khakimov, B.; Olsen, C.E.; Hansen, E.H.; Kuzina, V.; Ekstrom, C.T.; Hauser, T.; Bak, S.
UDP-glycosyltransferases from the UGT73C subfamily in Barbarea vulgaris catalyze sapogenin 3-O-glucosylation in saponin-mediated insect resistance
Plant Physiol.
160
1881-1895
2012
Barbarea vulgaris (K4GKX2), Barbarea vulgaris
brenda
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2.4.1.368 oleanolate 3-O-glucosyltransferase
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