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Information on EC 2.4.1.352 - glucosylglycerate phosphorylase and Organism(s) Meiothermus silvanus and UniProt Accession D7BAR0

for references in articles please use BRENDA:EC2.4.1.352
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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.352 glucosylglycerate phosphorylase
IUBMB Comments
The enzyme has been characterized from the bacterium Meiothermus silvanus.
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This record set is specific for:
Meiothermus silvanus
UNIPROT: D7BAR0
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The taxonomic range for the selected organisms is: Meiothermus silvanus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
glucosylglycerate phosphorylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
2-O-(alpha-D-glucopyranosyl)-D-glycerate:phosphate alpha-D-glucosyltransferase (configuration-retaining)
The enzyme has been characterized from the bacterium Meiothermus silvanus.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
alpha-D-glucopyranose 1-phosphate + D-glycerate
show the reaction diagram
-
-
-
r
alpha-D-glucopyranose 1-phosphate + D-glycerate
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
show the reaction diagram
-
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
alpha-D-glucopyranose 1-phosphate + D-glycerate
show the reaction diagram
-
-
-
r
alpha-D-glucopyranose 1-phosphate + D-glycerate
2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
show the reaction diagram
-
-
-
r
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.5
2-O-(alpha-D-glucopyranosyl)-D-glycerate
at pH 6.0 and 42°C
8.1
alpha-D-glucopyranose 1-phosphate
at pH 6.5 and 42°C
2.6
D-glycerate
at pH 6.5 and 42°C
2.5
phosphate
at pH 6.0 and 42°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.83
2-O-(alpha-D-glucopyranosyl)-D-glycerate
at pH 6.0 and 42°C
96
alpha-D-glucopyranose 1-phosphate
at pH 6.5 and 42°C
128
D-glycerate
at pH 6.5 and 42°C
0.98
phosphate
at pH 6.0 and 42°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.24
2-O-(alpha-D-glucopyranosyl)-D-glycerate
at pH 6.0 and 42°C
11.8
alpha-D-glucopyranose 1-phosphate
at pH 6.5 and 42°C
49.4
D-glycerate
at pH 6.5 and 42°C
0.4
phosphate
at pH 6.0 and 42°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
optimum pH in phosphorolytic direction
6.5
optimum pH in synthetic direction
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Franceus, J.; Pinel, D.; Desmet, T.
Glucosylglycerate phosphorylase, an enzyme with novel specificity involved in compatible solute metabolism
Appl. Environ. Microbiol.
83
e01434-17
2017
Meiothermus silvanus (D7BAR0), Meiothermus silvanus, Meiothermus silvanus ATCC 700542 (D7BAR0)
Manually annotated by BRENDA team