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IUBMB Comments The enzyme is involved in the biosynthesis of the linker region of the polymannose O-polysaccharide in the outer leaflet of the membrane of Escherichia coli serotypes O8, O9 and O9a. It has no activity with N -acetyl-α-D -glucosaminyl-diphospho-di trans ,octacis -undecaprenol (cf . EC 2.4.1.348 , N -acetyl-α-D -glucosaminyl-diphospho-di trans ,octacis -undecaprenol 3-α-mannosyltransferase).
The expected taxonomic range for this enzyme is: Escherichia coli
Synonyms WbdB, more
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WbdB
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2 GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = 2 GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
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MetaCyc
Escherichia coli serotype O:8 O antigen biosynthesis, Escherichia coli serotype O:9 O antigen biosynthesis, Escherichia coli serotype O:9a O antigen biosynthesis
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GDP-alpha-D-mannose:alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol 3-alpha-mannosyltransferase (configuration-retaining)
The enzyme is involved in the biosynthesis of the linker region of the polymannose O-polysaccharide in the outer leaflet of the membrane of Escherichia coli serotypes O8, O9 and O9a. It has no activity with N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol (cf. EC 2.4.1.348, N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol 3-alpha-mannosyltransferase).
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GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
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GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
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GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
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GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
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GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
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GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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WBDB_ECOLX
381
0
43868
Swiss-Prot
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x * 43900, calculated from amino acid sequence
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x * 43900, calculated from amino acid sequence
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x * 43900, calculated from amino acid sequence
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E294A
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the mutant shows a drastic reduction in activity
E302A
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the mutant shows about wild type activity
E294A
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the mutant shows a drastic reduction in activity
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E302A
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the mutant shows about wild type activity
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E294A
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the mutant shows a drastic reduction in activity
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E302A
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the mutant shows about wild type activity
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amylose resin column chromatography
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expressed in Escherichia coli Top10 cells
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Greenfield, L.; Richards, M.; Li, J.; Wakarchuk, W.; Lowary, T.; Whitfield, C.
Biosynthesis of the polymannose lipopolysaccharide O-antigens from Escherichia coli serotypes O8 and O9a requires a unique combination of single- and multiple-active site mannosyltransferases
J. Biol. Chem.
287
35078-35091
2012
Escherichia coli, Escherichia coli CWG634, Escherichia coli CWG636
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Greenfield, L.; Richards, M.; Vinogradov, E.; Wakarchuk, W.; Lowary, T.; Whitfield, C.
Domain organization of the polymerizing mannosyltransferases involved in synthesis of the Escherichia coli O8 and O9a lipopolysaccharide O-antigens
J. Biol. Chem.
287
38135-38149
2012
Escherichia coli, Escherichia coli CWG634
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Kido, N.; Sugiyama, T.; Yokochi, T.; Kobayashi, H.; Okawa, Y.
Synthesis of Escherichia coli O9a polysaccharide requires the participation of two domains of WbdA, a mannosyltransferase encoded within the wb gene cluster
Mol. Microbiol.
27
1213-1221
1998
Escherichia coli
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