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sitting drop vapor diffusion method at 20°C, crystals complexed with beta-1,2-Man2 and beta-1,2-Man3 are prepared by soaking in the cryoprotectant solution supplemented with 50 mM beta-1,2-Man2 or 10 mM beta-1,2-Man3. beta-1,2-Mannotriose is bound in a U-shape, interacting with a phosphate analog at both ends. Lin0857 has a unique dimer structure connected by a loop, and a significant open–close loop displacement iss observed for substrate entry. A long loop, which is exclusively present in Lin0857, covers the active site to limit the pocket size. A structural basis for substrate recognition and phosphorolysis is provided