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Information on EC 2.4.1.334 - 1,3-alpha-oligoglucan phosphorylase
for references in articles please use BRENDA:EC2.4.1.334
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.334 1,3-alpha-oligoglucan phosphorylase
IUBMB Comments
The enzyme, isolated from the bacterium Clostridium phytofermentans, catalyses a reversible reaction. Substrates for the phosphorolytic reaction are α-1,3-linked oligoglucans with a polymerisation degree of 3 or more. Nigerose (i.e. 3-O-α-D-glucopyranosyl-D-glucopyranose) is not phosphorylyzed but can serve as substrate in the reverse direction (cf. EC 2.4.1.279, nigerose phosphorylase).
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
showSynonyms
Cphy_3314, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Cphy_3314
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[(1->3)-alpha-D-glucosyl]n + phosphate = [(1->3)-alpha-D-glucosyl]n-1 + beta-D-glucose 1-phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
1,3-alpha-D-glucan:phosphate beta-D-glucosyltransferase
The enzyme, isolated from the bacterium Clostridium phytofermentans, catalyses a reversible reaction. Substrates for the phosphorolytic reaction are alpha-1,3-linked oligoglucans with a polymerisation degree of 3 or more. Nigerose (i.e. 3-O-alpha-D-glucopyranosyl-D-glucopyranose) is not phosphorylyzed but can serve as substrate in the reverse direction (cf. EC 2.4.1.279, nigerose phosphorylase).
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranosyl 1-phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Substrates: -
Products: -
Products: -
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
additional information
?
-
Substrates: the enzyme does not significantly phosphorylyze trehalose, kojibiose, nigerose, and maltose
Products: -
Products: -
?
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
Substrates: -
Products: -
Products: -
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
Substrates: -
Products: -
Products: -
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
Substrates: -
Products: -
Products: -
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
Substrates: -
Products: -
Products: -
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Substrates: 3-O-alpha-D-glucopyranosyl-D-glucopyranose i.e. nigerose
Products: -
Products: -
ir
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Substrates: 3-O-alpha-D-glucopyranosyl-D-glucopyranose i.e. nigerose
Products: -
Products: -
ir
kojibiose + beta-D-glucopyranose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
?
kojibiose + beta-D-glucopyranose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
?
maltose + beta-D-glucopyranose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
?
maltose + beta-D-glucopyranose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
Substrates: -
Products: -
Products: -
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
Substrates: -
Products: -
Products: -
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
Substrates: -
Products: -
Products: -
r
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
Substrates: -
Products: -
Products: -
r
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2.9
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate
3.1
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
8.3
beta-D-glucopyranose 1-phosphate
pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose
50
kojibiose
pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
3.8
maltose
pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
1 - 2
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
3.2
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate
2.6
phosphate
pH 7.0, 30°C, phosphorolysis reaction of 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose, release of beta-D-glucopyranose 1-phosphate
11
phosphate
pH 7.0, 30°C, phosphorolysis reaction of 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose, release of beta-D-glucopyranose 1-phosphate
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
8.5
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate
8.7
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
5.1
beta-D-glucopyranose 1-phosphate
pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose
3.5
kojibiose
pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
2.7
maltose
pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
11
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate
17
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.4
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
2.8
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
1.6
beta-D-glucopyranose 1-phosphate
pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose
0.069
kojibiose
pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
0.5
maltose
pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A9KSL6_LACP7
Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
805
0
92913
TrEMBL
-
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nihira, T.; Nishimoto, M.; Nakai, H.; Ohtsubo, K.; Kitaoka, M.
Characterization of two phosphorylases for alpha-1,3-oligoglucans from Clostridium phytofermentans
J. Appl. Glycosci.
61
59-66
2014
Lachnoclostridium phytofermentans (A9KSL6), Lachnoclostridium phytofermentans DSM 18823 (A9KSL6)
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brenda
EXTERNAL LINKS (specific for EC-Number 2.4.1.334)
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