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IUBMB Comments Isolated from the bacterium Saccharopolyspora erythraea . The enzyme is involved in the biosynthesis of the antibiotic erythromycin.
Synonyms
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dTDP-D-mycarose glycosyltransferase
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mycarosyl transferase
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dTDP-beta-L-mycarose + erythronolide B = dTDP + 3-alpha-L-mycarosylerythronolide B
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MetaCyc
erythromycin D biosynthesis
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dTDP-beta-L-mycarose:erythronolide B 3-beta-L-mycarosyltransferase
Isolated from the bacterium Saccharopolyspora erythraea. The enzyme is involved in the biosynthesis of the antibiotic erythromycin.
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dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-alpha-L-mycarosylerythronolide B
dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-beta-L-mycarosylerythronolide B
additional information
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Substrates: EryBV tolerates C5/C6 aglycon modifications and a small set of additional TDP-beta-L-sugars
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dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-alpha-L-mycarosylerythronolide B
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Substrates: -
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dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-alpha-L-mycarosylerythronolide B
Substrates: -
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dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-beta-L-mycarosylerythronolide B
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Substrates: the enzyme is involved in the erythromycin biosynthetic pathway
r
dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-beta-L-mycarosylerythronolide B
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Substrates: -
r
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dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-alpha-L-mycarosylerythronolide B
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Substrates: -
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dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-beta-L-mycarosylerythronolide B
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Substrates: the enzyme is involved in the erythromycin biosynthetic pathway
r
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additional information
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no requirement for an auxiliary activator protein
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brenda
enzyme catalyzes reaction of EC 2.4.1.277
UniProt
brenda
Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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malfunction
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deletion of eryBV gene results in the accumulation of 5-O-desosaminyl erythronolide B
physiological function
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the enzyme is involved in the erythromycin biosynthetic pathway
physiological function
coexpression of glycosyltransferase DesVII and its auxiliary partner DesVIII in a Saccharopolyspora erythraea EryBV deletion mutant leads to an increased erythromycin A yield by 15% in comparison to Saccharopolyspora erythraea wild-type. DesVII/DesVIII replaced EryCIII to upload D-desosamine to C5 position of 3-O-mycarosyl erythronolide B and also in vivo attache L-mycarose, not D-desosamine to C3 position of erythronolide B with a higher activity than EryBV. Expression of DesVII in EryCIII and EryBV-CIII mutant strains partially restores the erythromycin A production, however, no erythromycin A is detected while DesVII is expressed in the EryBV mutant strain
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ERYBV_SACER
415
0
45563
Swiss-Prot
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DES7_STRVZ
426
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46423
Swiss-Prot
Secretory Pathway (Reliability: 2 )
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45500
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x * 45500, calculated from amino acid sequence
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x * 45500, calculated from amino acid sequence
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-80°C, stable for more than three months
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expressed in Streptomyces lividans
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Summers, R.G.; Donadio, S.; Staver, M.J.; Wendt-Pienkowski, E.; Hutchinson, C.R., Katz, L.
Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production
Microbiology
143
3251-3262
1997
Saccharopolyspora erythraea
brenda
Zhang, C.; Fu, Q.; Albermann, C.; Li, L.; Thorson, J.S.
The in vitro characterization of the erythronolide mycarosyltransferase EryBV and its utility in macrolide diversification
Chembiochem
8
385-390
2007
Saccharopolyspora erythraea
brenda
Basnet, D.B.; Park, J.W.; Yoon, Y.J.
Combinatorial biosynthesis of 5-O-desosaminyl erythronolide A as a potent precursor of ketolide antibiotics
J. Biotechnol.
135
92-96
2008
Saccharopolyspora erythraea
brenda
Wu, H.; Li, W.; Xin, C.; Zhang, C.; Wang, Y.; Ren, S.; Ren, M.; Zhao, W.; Yuan, L.; Xu, Z.; Yuan, H.; Geng, M.; Zhang, L.; Weaver, D.T.; Zhang, B.
In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea
Appl. Microbiol. Biotechnol.
100
2257-2266
2016
Streptomyces venezuelae (Q9ZGH7)
brenda
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