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EC Tree
IUBMB Comments Isolated from the bacterium Saccharopolyspora erythraea. The enzyme is involved in the biosynthesis of the antibiotic erythromycin.
The enzyme appears in viruses and cellular organisms
Synonyms
DesVII, dTDP-D-mycarose glycosyltransferase, EryBV, mycarosyl transferase,
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dTDP-D-mycarose glycosyltransferase
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mycarosyl transferase
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dTDP-beta-L-mycarose + erythronolide B = dTDP + 3-alpha-L-mycarosylerythronolide B
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dTDP-beta-L-mycarose:erythronolide B 3-beta-L-mycarosyltransferase
Isolated from the bacterium Saccharopolyspora erythraea. The enzyme is involved in the biosynthesis of the antibiotic erythromycin.
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dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-alpha-L-mycarosylerythronolide B
dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-beta-L-mycarosylerythronolide B
additional information
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EryBV tolerates C5/C6 aglycon modifications and a small set of additional TDP-beta-L-sugars
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dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-alpha-L-mycarosylerythronolide B
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dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-alpha-L-mycarosylerythronolide B
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dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-beta-L-mycarosylerythronolide B
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r
dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-beta-L-mycarosylerythronolide B
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the enzyme is involved in the erythromycin biosynthetic pathway
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r
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dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-alpha-L-mycarosylerythronolide B
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dTDP-beta-L-mycarose + erythronolide B
dTDP + 3-beta-L-mycarosylerythronolide B
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the enzyme is involved in the erythromycin biosynthetic pathway
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r
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additional information
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no requirement for an auxiliary activator protein
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brenda
enzyme catalyzes reaction of EC 2.4.1.277
UniProt
brenda
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malfunction
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deletion of eryBV gene results in the accumulation of 5-O-desosaminyl erythronolide B
physiological function
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the enzyme is involved in the erythromycin biosynthetic pathway
physiological function
coexpression of glycosyltransferase DesVII and its auxiliary partner DesVIII in a Saccharopolyspora erythraea EryBV deletion mutant leads to an increased erythromycin A yield by 15% in comparison to Saccharopolyspora erythraea wild-type. DesVII/DesVIII replaced EryCIII to upload D-desosamine to C5 position of 3-O-mycarosyl erythronolide B and also in vivo attache L-mycarose, not D-desosamine to C3 position of erythronolide B with a higher activity than EryBV. Expression of DesVII in EryCIII and EryBV-CIII mutant strains partially restores the erythromycin A production, however, no erythromycin A is detected while DesVII is expressed in the EryBV mutant strain
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ERYBV_SACER
415
0
45563
Swiss-Prot
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DES7_STRVZ
426
0
46423
Swiss-Prot
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45500
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x * 45500, calculated from amino acid sequence
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x * 45500, calculated from amino acid sequence
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-80°C, stable for more than three months
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expressed in Streptomyces lividans
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Summers, R.G.; Donadio, S.; Staver, M.J.; Wendt-Pienkowski, E.; Hutchinson, C.R., Katz, L.
Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production
Microbiology
143
3251-3262
1997
Saccharopolyspora erythraea
brenda
Zhang, C.; Fu, Q.; Albermann, C.; Li, L.; Thorson, J.S.
The in vitro characterization of the erythronolide mycarosyltransferase EryBV and its utility in macrolide diversification
Chembiochem
8
385-390
2007
Saccharopolyspora erythraea
brenda
Basnet, D.B.; Park, J.W.; Yoon, Y.J.
Combinatorial biosynthesis of 5-O-desosaminyl erythronolide A as a potent precursor of ketolide antibiotics
J. Biotechnol.
135
92-96
2008
Saccharopolyspora erythraea
brenda
Wu, H.; Li, W.; Xin, C.; Zhang, C.; Wang, Y.; Ren, S.; Ren, M.; Zhao, W.; Yuan, L.; Xu, Z.; Yuan, H.; Geng, M.; Zhang, L.; Weaver, D.T.; Zhang, B.
In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea
Appl. Microbiol. Biotechnol.
100
2257-2266
2016
Streptomyces venezuelae (Q9ZGH7)
brenda
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2.4.1.328 erythronolide mycarosyltransferase
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