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IUBMB Comments Isolated from the bacterium Streptomyces galilaeus. Forms a complex with its accessory protein AknT, and has very low activity in its absence. The enzyme can also use dTDP-2-deoxy-beta-L-fucose. Involved in the biosynthesis of other aclacinomycins.
The enzyme appears in viruses and cellular organisms
Synonyms
aklavinone 7-beta-L-rhodosaminyltransferase, AknS, AknS/AknT, AknT, Gtf,
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aklavinone 7-beta-L-rhodosaminyltransferase
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AknS/AknT
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dTDP-beta-L-rhodosamine + aklavinone = dTDP + aclacinomycin T
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dTDP-beta-L-rhodosamine:aklavinone 7-alpha-L-rhodosaminyltransferase (configuration-inverting)
Isolated from the bacterium Streptomyces galilaeus. Forms a complex with its accessory protein AknT, and has very low activity in its absence. The enzyme can also use dTDP-2-deoxy-beta-L-fucose. Involved in the biosynthesis of other aclacinomycins.
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dTDP-4-amino-2-deoxyrhamnose + aklavinone
dTDP + 4-O-(2,4-dideoxy-4-amino-alpha-L-rhamnopyranosyl)aklavinone
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-
-
-
?
dTDP-4-amino-2-deoxyrhamnose + aklavinone
dTDP + 4-O-(2,4-dideoxy-4-amino-alpha-L-rhamnosyl)aklavinone
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-
-
-
?
dTDP-beta-L-rhodosamine + aklavinone
dTDP + aclacinomycin T
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-
-
-
?
dTDP-beta-L-rhodosamine + aklavinone
dTDP + rhodosaminyl-aklavinone
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-
-
-
?
dTDP-L-2-deoxyfucose + aklavinone
dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)aklavinone
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-
-
-
?
dTDP-L-2-deoxyfucose + epsilon-rhodomycinone
dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)-epsilon-rhodomycinone
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-
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?
dTDP-L-daunosamine + aklavinone
dTDP + ?
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?
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dTDP-beta-L-rhodosamine + aklavinone
dTDP + aclacinomycin T
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-
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?
dTDP-beta-L-rhodosamine + aklavinone
dTDP + rhodosaminyl-aklavinone
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?
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AKnT protein
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the enzyme AknS has very low activity in the absence of the adjacently encoded AknT activator protein. At a 3:1 ratio in the 0.0025-0.01 mM range, AknT stimulates AknS kcat by 40fold for transfer of L-2-deoxyfucose to aklavinone
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AKnT protein
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the optimal ratio of AknS to AknT is 1:3
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0.0031 - 0.013
aklavinone
0.4072
dTDP-4-amino-2-deoxyrhamnose
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in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
0.28 - 0.349
dTDP-beta-L-rhodosamine
0.156 - 0.2878
dTDP-L-2-deoxyfucose
0.046
epsilon-rhodomycinone
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with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
0.0031
aklavinone
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with dTDP-4-amino-2-deoxyrhamnose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
0.0035
aklavinone
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with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
0.005
aklavinone
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in the presence of activator protein AknT, at pH 7.5 and 25°C
0.013
aklavinone
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in the absence of activator protein AknT, at pH 7.5 and 25°C
0.28
dTDP-beta-L-rhodosamine
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in the presence of activator protein AknT, at pH 7.5 and 25°C
0.349
dTDP-beta-L-rhodosamine
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in the absence of activator protein AknT, at pH 7.5 and 25°C
0.156
dTDP-L-2-deoxyfucose
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with epsilon-rhodomycinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
0.2878
dTDP-L-2-deoxyfucose
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with aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
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0.003
dTDP-4-amino-2-deoxyrhamnose
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in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
0.0083 - 0.16
dTDP-beta-L-rhodosamine
0.001 - 0.004
dTDP-L-2-deoxyfucose
0.001
epsilon-rhodomycinone
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with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
0.003
aklavinone
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with dTDP-4-amino-2-deoxyrhamnose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
0.004
aklavinone
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with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
0.0083
dTDP-beta-L-rhodosamine
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in the absence of activator protein AknT, at pH 7.5 and 25°C
0.16
dTDP-beta-L-rhodosamine
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at pH 7.5 and 25°C
0.001
dTDP-L-2-deoxyfucose
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with epsilon-rhodomycinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
0.004
dTDP-L-2-deoxyfucose
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with aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
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brenda
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AKNS_STRGJ
443
0
47454
Swiss-Prot
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A0A7W4ZTQ9_9ACTN
426
0
46646
TrEMBL
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A0A7X0KFT6_9ACTN
420
0
46024
TrEMBL
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Ni-NTA affinity column chromatography, and gel filtration
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expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli XL1 Blue MRF' cells
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Lu, W.; Leimkuhler, C.; Gatto, G.J. Jr.
Kruger, R.G.; Oberthür, M.; Kahne, D.; Walsh, C.T.: AknT is an activating protein for the glycosyltransferase AknS in L-aminodeoxysugar transfer to the aglycone of aclacinomycin A
Chem. Biol.
12
527-534
2005
Streptomyces galilaeus
brenda
Leimkuhler, C.; Fridman, M.; Lupoli, T.; Walker, S.; Walsh, C.T.; Kahne, D.
Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A
J. Am. Chem. Soc.
129
10546-10550
2007
Streptomyces galilaeus
brenda
Raety, K.; Kunnari, T.; Hakala, J.; Maentsaelae, P.; Ylihonko, K.
A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin
Mol. Gen. Genet.
264
164-172
2000
Streptomyces galilaeus
brenda
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2.4.1.326 aklavinone 7-L-rhodosaminyltransferase
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