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Information on EC 2.4.1.320 - 1,4-beta-mannosyl-N-acetylglucosamine phosphorylase and Organism(s) Bacteroides thetaiotaomicron and UniProt Accession Q8A8Y4

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IUBMB Comments
The enzyme isolated from the anaerobic bacterium Bacteroides thetaiotaomicron is involved in the degradation of host-derived N-glycans.
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This record set is specific for:
Bacteroides thetaiotaomicron
UNIPROT: Q8A8Y4
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The taxonomic range for the selected organisms is: Bacteroides thetaiotaomicron
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
bt1033, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate = N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
sequential Bi Bi mechanism
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine:phosphate alpha-D-mannosyltransferase
The enzyme isolated from the anaerobic bacterium Bacteroides thetaiotaomicron is involved in the degradation of host-derived N-glycans.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
the enzyme is involved in the degradation of host-derived N-glycans
-
-
r
N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate
beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine + phosphate
show the reaction diagram
-
-
-
r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
show the reaction diagram
-
-
-
r
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
show the reaction diagram
-
-
-
r
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
show the reaction diagram
-
-
-
r
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
the enzyme is involved in the degradation of host-derived N-glycans
-
-
r
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
show the reaction diagram
-
-
-
r
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.1
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine
pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction
2.6
alpha-D-mannose 1-phosphate
pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction
27
N,N'-diacetylchitobiose
pH 5.5, 30°C, synthetic reaction
3.8
N-acetyl-D-glucosamine
pH 5.5, 30°C, synthetic reaction
0.6
phosphate
pH 5.5, 30°C, cosubstrate: 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine, phosphorolytic reaction
2.7
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine
pH 5.5, 30°C
2.6
alpha-D-mannose 1-phosphate
pH 5.5, 30°C
3.8
N-acetyl-D-glucosamine
pH 5.5, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
240
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine
pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction
25
alpha-D-mannose 1-phosphate
pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction
22
N,N'-diacetylchitobiose
pH 5.5, 30°C, synthetic reaction
37
N-acetyl-D-glucosamine
pH 5.5, 30°C, synthetic reaction
240
phosphate
pH 5.5, 30°C, cosubstrate: 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine, phosphorolytic reaction
22
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine
pH 5.5, 30°C
25
alpha-D-mannose 1-phosphate
pH 5.5, 30°C
37
N-acetyl-D-glucosamine
pH 5.5, 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
110
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine
pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction
9.6
alpha-D-mannose 1-phosphate
pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction
1.9
beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine
pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction
0.8
N,N'-diacetylchitobiose
pH 5.5, 30°C, synthetic reaction
9.6
N-acetyl-D-glucosamine
pH 5.5, 30°C, synthetic reaction
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
258
pH 5.5, 30°C, substrate: N-acetyl-D-glucosamine
30
pH 5.5, 30°C, substrate: N,N'-diacetylchitobiose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme is involved in the degradation of host-derived N-glycans
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
4 * 35000, SDS-PAGE
37823
4 * 37823, calculated from sequence
35000
4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence
37823
4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
homotetramer
4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 10.5
4°C, 24 h, stable
725534
4.5 - 10.5
4°C, 24 h stable
725534
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expressed in Escherichia coli DH5alpha
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nihira, T.; Suzuki, E.; Kitaoka, M.; Nishimoto, M.; Ohtsubo, K.; Nakai, H.
Discovery of beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase involved in the metabolism of N-glycans
J. Biol. Chem.
288
27366-27374
2013
Bacteroides thetaiotaomicron (Q8A8X4), Bacteroides thetaiotaomicron (Q8A8Y4), Bacteroides thetaiotaomicron VPI-5482 (Q8A8X4), Bacteroides thetaiotaomicron VPI-5482 (Q8A8Y4), Bacteroides thetaiotaomicron VPI-5482
Manually annotated by BRENDA team