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IUBMB CommentsThe enzyme isolated from the anaerobic bacterium Bacteroides thetaiotaomicron is involved in the degradation of host-derived N-glycans.
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
bt1033,
more
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beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase
beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase
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beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase
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BT1033
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4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate = N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate = N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
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4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate = N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
sequential Bi Bi mechanism
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate = N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
sequential Bi Bi mechanism
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4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine:phosphate alpha-D-mannosyltransferase
The enzyme isolated from the anaerobic bacterium Bacteroides thetaiotaomicron is involved in the degradation of host-derived N-glycans.
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4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate
beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
additional information
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4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
the enzyme is involved in the degradation of host-derived N-glycans
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r
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
the enzyme is involved in the degradation of host-derived N-glycans
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r
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
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r
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
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r
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
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-
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r
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
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r
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
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r
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
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r
N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate
beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine + phosphate
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r
N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate
beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine + phosphate
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r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
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r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
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r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
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r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
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r
additional information
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very low activity with D-glucose or D-mannose as acceptor and alpha-D-mannose 1-phosphate as donor
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additional information
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very low activity with D-glucose or D-mannose as acceptor and alpha-D-mannose 1-phosphate as donor
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additional information
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very low activity with D-glucose or D-mannose as acceptor and alpha-D-mannose 1-phosphate as donor
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additional information
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very low activity with D-glucose or D-mannose as acceptor and alpha-D-mannose 1-phosphate as donor
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additional information
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very low activity with D-glucose or D-mannose as acceptor and alpha-D-mannose 1-phosphate as donor
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
the enzyme is involved in the degradation of host-derived N-glycans
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r
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
the enzyme is involved in the degradation of host-derived N-glycans
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r
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
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r
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
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r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
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r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
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r
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2.1
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine
pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction
2.7
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine
pH 5.5, 30°C
2.6
alpha-D-mannose 1-phosphate
27
N,N'-diacetylchitobiose
pH 5.5, 30°C, synthetic reaction
3.8
N-acetyl-D-glucosamine
0.6
phosphate
pH 5.5, 30°C, cosubstrate: 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine, phosphorolytic reaction
2.6
alpha-D-mannose 1-phosphate
pH 5.5, 30°C
2.6
alpha-D-mannose 1-phosphate
pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction
3.8
N-acetyl-D-glucosamine
pH 5.5, 30°C
3.8
N-acetyl-D-glucosamine
pH 5.5, 30°C, synthetic reaction
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240
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine
pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction
22
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine
pH 5.5, 30°C
25
alpha-D-mannose 1-phosphate
22
N,N'-diacetylchitobiose
pH 5.5, 30°C, synthetic reaction
37
N-acetyl-D-glucosamine
240
phosphate
pH 5.5, 30°C, cosubstrate: 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine, phosphorolytic reaction
25
alpha-D-mannose 1-phosphate
pH 5.5, 30°C
25
alpha-D-mannose 1-phosphate
pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction
37
N-acetyl-D-glucosamine
pH 5.5, 30°C
37
N-acetyl-D-glucosamine
pH 5.5, 30°C, synthetic reaction
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110
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine
pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction
9.6
alpha-D-mannose 1-phosphate
pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction
1.9
beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine
pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction
0.8
N,N'-diacetylchitobiose
pH 5.5, 30°C, synthetic reaction
9.6
N-acetyl-D-glucosamine
pH 5.5, 30°C, synthetic reaction
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258
pH 5.5, 30°C, substrate: N-acetyl-D-glucosamine
30
pH 5.5, 30°C, substrate: N,N'-diacetylchitobiose
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5.5
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5.5
phosphorolytic reaction
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UniProt
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UniProt
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brenda
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brenda
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physiological function
the enzyme is involved in the degradation of host-derived N-glycans
physiological function
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the enzyme is involved in the degradation of host-derived N-glycans
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
BMAGP_BACTN
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)
322
0
36759
Swiss-Prot
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A0A7U9QYN6_9FIRM
357
0
40605
TrEMBL
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A0A7J0BA63_9BACT
318
0
35948
TrEMBL
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A0A645I6M9_9ZZZZ
150
0
16882
TrEMBL
other Location (Reliability: 4)
A0A2H5XLN8_9BACT
364
0
40530
TrEMBL
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A0A2H5Y9N3_9CHLR
291
0
33525
TrEMBL
-
A0A140L9R3_9FIRM
1183
0
135881
TrEMBL
-
A0A2H5V0Y9_9ARCH
317
0
36505
TrEMBL
-
A0A2S2DXB3_9BACT
364
0
41432
TrEMBL
-
A0A645FRH4_9ZZZZ
192
0
21446
TrEMBL
other Location (Reliability: 2)
A0A6V6YHD6_9FLAO
Flavobacterium panici
321
0
36300
TrEMBL
-
A0A162MLB8_9FIRM
1163
0
134105
TrEMBL
-
A0A2H6AIK7_9BACT
103
0
11620
TrEMBL
-
A0A7U9RS25_9FIRM
326
0
37018
TrEMBL
-
A0A645F2R8_9ZZZZ
302
0
34487
TrEMBL
other Location (Reliability: 4)
A0A644YUZ6_9ZZZZ
308
0
34321
TrEMBL
other Location (Reliability: 3)
A0A7W8MWU2_9BACI
341
0
38869
TrEMBL
-
A0A8I2BY04_9BACL
349
0
39143
TrEMBL
-
A0A4P6ZC33_9FLAO
359
0
40567
TrEMBL
-
A0A4P6ZC53_9FLAO
486
0
55890
TrEMBL
-
A0A5J4E834_9BACT
499
0
56876
TrEMBL
-
A0A1G4G2W7_9BACT
488
0
55601
TrEMBL
-
A0A840CZ05_9BACT
333
0
37688
TrEMBL
-
A0A2H5YH71_9BACT
300
0
35065
TrEMBL
-
A0A841ERS8_9BACL
345
0
38737
TrEMBL
-
A0A2H6A888_9BACT
639
0
73095
TrEMBL
-
A0A7I9ZZW2_9BACE
322
0
36704
TrEMBL
-
A0A140L124_9FIRM
302
0
34772
TrEMBL
-
A0A7J0B0U6_9BACT
Alistipes sp
322
0
36704
TrEMBL
-
A0A7W7IYH9_9FLAO
321
0
36463
TrEMBL
-
A0A2H5WSU1_9BACT
354
0
40030
TrEMBL
-
A0A2H5ZU49_9BACT
344
0
39438
TrEMBL
-
A0A645HV11_9ZZZZ
173
0
19643
TrEMBL
other Location (Reliability: 2)
A0A221UZS5_9FLAO
321
0
36413
TrEMBL
-
A0A839THX4_9BACL
335
0
37661
TrEMBL
-
A0A369QPT9_9BACT
390
0
43745
TrEMBL
-
A0A221USF4_9FLAO
349
0
39116
TrEMBL
-
A0A2H5XLN3_9BACT
343
0
38395
TrEMBL
-
A0A7W5CCX0_9BACL
335
0
37674
TrEMBL
-
A0A7W7AQU5_9BACT
332
0
37785
TrEMBL
-
A0A645GXE9_9ZZZZ
206
0
22961
TrEMBL
other Location (Reliability: 4)
A0A140LBA3_9FIRM
307
0
35056
TrEMBL
-
A0A2H5XLL8_9BACT
715
0
79913
TrEMBL
-
A0A7J0AAY4_9BACT
322
0
36512
TrEMBL
-
A0A8E2D408_9PORP
334
0
38096
TrEMBL
-
A0A7J0A8D4_9BACE
172
0
19082
TrEMBL
-
A0A841N900_9FLAO
323
0
36783
TrEMBL
-
A0A840D020_9BACE
326
0
37054
TrEMBL
-
A0A841GQP8_9BACT
312
0
36250
TrEMBL
-
A0A7U7CYW3_9FLAO
323
0
36698
TrEMBL
-
A0A644UVF6_9ZZZZ
334
0
38110
TrEMBL
other Location (Reliability: 1)
A0A5J4PNU1_9ZZZZ
252
0
28303
TrEMBL
other Location (Reliability: 1)
A0A4Q7DE53_9MICO
360
0
39104
TrEMBL
-
A0A5S9RC04_MYCVN
492
0
53173
TrEMBL
-
A0A5K7XCW3_9BACT
355
0
39767
TrEMBL
-
A0A2H6A870_9BACT
395
0
45574
TrEMBL
-
A0A644WHX6_9ZZZZ
319
0
36192
TrEMBL
other Location (Reliability: 3)
A0A2H5WKQ7_9BACT
355
0
39830
TrEMBL
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Q8A8X4_BACTN
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)
546
0
60933
TrEMBL
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35000
4 * 35000, SDS-PAGE
35000
4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence
37823
4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence
37823
4 * 37823, calculated from sequence
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homotetramer
4 * 35000, SDS-PAGE
homotetramer
4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence
homotetramer
4 * 37823, calculated from sequence
homotetramer
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4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence
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homotetramer
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4 * 35000, SDS-PAGE
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homotetramer
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4 * 37823, calculated from sequence
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4.5 - 10.5
4°C, 24 h, stable
725534
4.5 - 10.5
4°C, 24 h stable
725534
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55
15 min, stable up to
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expressed in Escherichia coli DH5alpha
expression in Escherichia coli
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Nihira, T.; Suzuki, E.; Kitaoka, M.; Nishimoto, M.; Ohtsubo, K.; Nakai, H.
Discovery of beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase involved in the metabolism of N-glycans
J. Biol. Chem.
288
27366-27374
2013
Bacteroides thetaiotaomicron (Q8A8X4), Bacteroides thetaiotaomicron (Q8A8Y4), Bacteroides thetaiotaomicron VPI-5482 (Q8A8X4), Bacteroides thetaiotaomicron VPI-5482 (Q8A8Y4), Bacteroides thetaiotaomicron VPI-5482
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