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Information on EC 2.4.1.315 - diglucosyl diacylglycerol synthase (1,6-linking) and Organism(s) Mycoplasma genitalium and UniProt Accession Q9ZB73

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.315 diglucosyl diacylglycerol synthase (1,6-linking)
IUBMB Comments
The enzyme is found in several bacterial species. The enzyme from Bacillus subtilis is specific for glucose . The enzyme from Mycoplasma genitalium can incoporate galactose with similar efficiency, but forms mainly 1,2-diacyl-diglucopyranosyl-sn-glycerol in vivo . The enzyme from Staphylococcus aureus can also form glucosyl-glycero-3-phospho-(1'-sn-glycerol) .
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Mycoplasma genitalium
UNIPROT: Q9ZB73
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The taxonomic range for the selected organisms is: Mycoplasma genitalium
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
mg517, ugt106b1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol 6-glucosyltransferase
The enzyme is found in several bacterial species. The enzyme from Bacillus subtilis is specific for glucose [1]. The enzyme from Mycoplasma genitalium can incoporate galactose with similar efficiency, but forms mainly 1,2-diacyl-diglucopyranosyl-sn-glycerol in vivo [3]. The enzyme from Staphylococcus aureus can also form glucosyl-glycero-3-phospho-(1'-sn-glycerol) [2].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-galactose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
9% of the specific activity with 1,2-diacyl-sn-glycerol
-
-
?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
2.2% of the specific activity with 1,2-diacyl-sn-glycerol
-
-
?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
-
main product
-
?
UDP-alpha-D-glucose + 1,2-diacyl-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol + UDP
show the reaction diagram
-
-
-
?
additional information
?
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dioleoylphosphatidylglycerol
kcat linearly increases with dioleoylphosphatidylglycerol concentration
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09
1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
donor UDP-alpha-D-galactose, pH 8.0, 28°C
0.27
1,2-diacyl-sn-glycerol
donor UDP-alpha-D-galactose, pH 8.0, 28°C
0.234 - 0.479
UDP-alpha-D-galactose
0.05 - 0.087
UDP-alpha-D-glucose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023
1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
donor UDP-alpha-D-galactose, pH 8.0, 28°C
1.13
1,2-diacyl-sn-glycerol
donor UDP-alpha-D-galactose, pH 8.0, 28°C
0.03 - 1.1
UDP-alpha-D-galactose
0.004 - 0.27
UDP-alpha-D-glucose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
donor UDP-alpha-D-galactose, pH 8.0, 28°C
4
1,2-diacyl-sn-glycerol
donor UDP-alpha-D-galactose, pH 8.0, 28°C
0.063 - 5
UDP-alpha-D-galactose
0.08 - 3.1
UDP-alpha-D-glucose
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ATCC 33530
Uniprot
Manually annotated by BRENDA team
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Andres, E.; Martinez, N.; Planas, A.
Expression and characterization of a Mycoplasma genitalium glycosyltransferase in membrane glycolipid biosynthesis: Potential target against Mycoplasma infections
J. Biol. Chem.
286
35367-35379
2011
Mycoplasma genitalium (Q9ZB73), Mycoplasma genitalium
Manually annotated by BRENDA team