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Information on EC 2.4.1.315 - diglucosyl diacylglycerol synthase (1,6-linking) and Organism(s) Bacillus subtilis and UniProt Accession P54166

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.315 diglucosyl diacylglycerol synthase (1,6-linking)
IUBMB Comments
The enzyme is found in several bacterial species. The enzyme from Bacillus subtilis is specific for glucose . The enzyme from Mycoplasma genitalium can incoporate galactose with similar efficiency, but forms mainly 1,2-diacyl-diglucopyranosyl-sn-glycerol in vivo . The enzyme from Staphylococcus aureus can also form glucosyl-glycero-3-phospho-(1'-sn-glycerol) .
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Bacillus subtilis
UNIPROT: P54166
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Word Map
The taxonomic range for the selected organisms is: Bacillus subtilis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
mg517, ugt106b1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DGlcDAG synthase
ambiguous
MGlcDAG (1->6) glucosyltransferase
-
monoglucosyl diacylglycerol (1->6) glucosyltransferase
-
UDP-glucose:diacylglycerol glucosyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-[beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
(1)
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl]-sn-glycerol = 1,2-diacyl-3-O-[beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
(2)
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol 6-glucosyltransferase
The enzyme is found in several bacterial species. The enzyme from Bacillus subtilis is specific for glucose [1]. The enzyme from Mycoplasma genitalium can incoporate galactose with similar efficiency, but forms mainly 1,2-diacyl-diglucopyranosyl-sn-glycerol in vivo [3]. The enzyme from Staphylococcus aureus can also form glucosyl-glycero-3-phospho-(1'-sn-glycerol) [2].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
-
-
-
?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
can only use UDP-glucose as sugar donor
-
-
?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
can only use UDP-glucose as sugar donor
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
strong expression in the membrane and inclusion body fraction
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43600
x * 43600, calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Jorasch, P.; Wolter, F.P.; Zahringer, U.; Heinz, E.
A UDP glucosyltransferase from Bacillus subtilis successively transfers up to four glucose residues to 1,2-diacylglycerol: expression of ypfP in Escherichia coli and structural analysis of its reaction products
Mol. Microbiol.
29
419-430
1998
Bacillus subtilis (P54166), Bacillus subtilis 60015 (P54166)
Manually annotated by BRENDA team