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Information on EC 2.4.1.308 - GDP-Fuc:beta-D-Gal-1,3-alpha-D-GalNAc-1,3-alpha-GalNAc-diphosphoundecaprenol alpha-1,2-fucosyltransferase
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2.4.1.308 GDP-Fuc:beta-D-Gal-1,3-alpha-D-GalNAc-1,3-alpha-GalNAc-diphosphoundecaprenol alpha-1,2-fucosyltransferaseIUBMB Comments
The enzyme is involved in the biosynthesis of the O-polysaccharide repeating unit of the bacterium Escherichia coli serotype O86.
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The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
WbnK, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
WbnK
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol = GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
GDP-beta-L-fucose:beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol alpha-1,2-fucosyltransferase
The enzyme is involved in the biosynthesis of the O-polysaccharide repeating unit of the bacterium Escherichia coli serotype O86.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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?
GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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?
GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
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?
GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
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?
GDP-beta-L-fucose + beta-D-Gal-OMe
?
15% of the activity compared to beta-Gal-(1->3)-alpha-GalNAc-OMe
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?
GDP-beta-L-fucose + beta-D-Gal-OMe
?
15% of the activity compared to beta-Gal-(1->3)-alpha-GalNAc-OMe
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?
additional information
?
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WbnK requires a terminal beta-1,3-linked Gal sugar sequence as acceptor
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?
additional information
?
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WbnK requires a terminal beta-1,3-linked Gal sugar sequence as acceptor
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?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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-
?
GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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?
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
the enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
physiological function
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the enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A644UUW6_9ZZZZ
299
0
34027
TrEMBL
other Location (Reliability: 4)
A0A644T6A4_9ZZZZ
298
0
35307
TrEMBL
other Location (Reliability: 4)
A0A644WSJ0_9ZZZZ
284
1
33563
TrEMBL
other Location (Reliability: 2)
A0A5J4SD00_9ZZZZ
298
0
35282
TrEMBL
Mitochondrion (Reliability: 4)
A0A5J4SRT5_9ZZZZ
293
0
33846
TrEMBL
other Location (Reliability: 5)
A0A221K4F3_9RHOB
283
0
31853
TrEMBL
-
A0A5J4SIP6_9ZZZZ
281
0
33997
TrEMBL
other Location (Reliability: 4)
A0A5J4QE75_9ZZZZ
282
0
33644
TrEMBL
Mitochondrion (Reliability: 5)
A0A5J4Q407_9ZZZZ
100
0
12171
TrEMBL
other Location (Reliability: 2)
A0A645HLH3_9ZZZZ
127
0
15286
TrEMBL
other Location (Reliability: 2)
A0A5J4SAU2_9ZZZZ
296
0
35401
TrEMBL
Mitochondrion (Reliability: 3)
A0A5J4RDT4_9ZZZZ
282
0
33756
TrEMBL
Mitochondrion (Reliability: 5)
A0A5M8P597_9BACT
330
0
38960
TrEMBL
-
A0A645IX30_9ZZZZ
140
0
16499
TrEMBL
other Location (Reliability: 4)
A0A2R8BIP4_9RHOB
280
0
31704
TrEMBL
-
A0A644SZN0_9ZZZZ
295
0
33500
TrEMBL
other Location (Reliability: 3)
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
synthesis
stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Biosynthetic pathway of B antigen is the same in Escherichia coli O86 as in humans, thus providing us an attractive way of synthesizing blood group B antigen with microbial glycosyltransferases
synthesis
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stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Biosynthetic pathway of B antigen is the same in Escherichia coli O86 as in humans, thus providing us an attractive way of synthesizing blood group B antigen with microbial glycosyltransferases
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yi, W.; Shao, J.; Zhu, L.; Li, M.; Singh, M.; Lu, Y.; Lin, S.; Li, H.; Ryu, K.; Shen, J.; Guo, H.; Yao, Q.; Bush, C.A.; Wang, P.G.
Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide
J. Am. Chem. Soc.
127
2040-2041
2005
Escherichia coli (Q58YV9), Escherichia coli O86 (Q58YV9)
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Woodward, R.; Yi, W.; Li, L.; Zhao, G.; Eguchi, H.; Sridhar, P.R.; Guo, H.; Song, J.K.; Motari, E.; Cai, L.; Kelleher, P.; Liu, X.; Han, W.; Zhang, W.; Ding, Y.; Li, M.; Wang, P.G.
In vitro bacterial polysaccharide biosynthesis: defining the functions of Wzy and Wzz
Nat. Chem. Biol.
6
418-423
2010
Escherichia coli (Q58YV9), Escherichia coli O86:B7 (Q58YV9)
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