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Information on EC 2.4.1.304 - UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase
for references in articles please use BRENDA:EC2.4.1.304
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.304 UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase
IUBMB Comments
The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of the bacterium Shigella boydii B14. The activity is stimulated by Mn2+ or to a lesser extent by Mg2+, Ca2+, Ni2+ or Pb2+.
The expected taxonomic range for this enzyme is: Shigella boydii
Reaction Schemes
showSynonyms
UDP-Gal:GlcNAc-pyrophosphate-lipid beta-1,4-galactosyltransferase, UDP-Gal:GlcNAc-R 1,4-Gal-transferase, WfeD, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
UDP-Gal:GlcNAc-pyrophosphate-lipid beta-1,4-galactosyltransferase
UDP-Gal:GlcNAc-R 1,4-Gal-transferase
WfeD
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
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-
-
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-galactose:N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol beta-1,4-galactosyltransferase
The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of the bacterium Shigella boydii B14. The activity is stimulated by Mn2+ or to a lesser extent by Mg2+, Ca2+, Ni2+ or Pb2+.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-phenoxyundecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-phenoxyundecanol
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphosphononanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphononanol
-
Substrates: the enzyme requires diphosphate in the acceptor substrate
Products: -
Products: -
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-phospho-phenoxyundecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-phospho-phenoxyundecanol
additional information
?
-
-
Substrates: no activity with: alpha- and beta-anomers of GlcNAc-benzyl, beta-anomers of GlcNBu-benzyl, GlcNAc-naphthyl, GlcNAc-isoquinolinyl, as well as GlcNAc-alpha-OCOCH2-COO(CH2)11-OPh and alpha- and beta-anomers of GlcNAc-(CH2)11-OPh
Products: -
Products: -
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
Substrates: the O-antigen in the outer membrane of Gram-negative bacteria contains many repeats of an oligosaccharide unit. WfeD catalyzes a step of the repeating-unit assembly in Shigella boydii B14
Products: -
Products: -
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
Substrates: the O-antigen in the outer membrane of Gram-negative bacteria contains many repeats of an oligosaccharide unit. WfeD catalyzes a step of the repeating-unit assembly in Shigella boydii B14
Products: -
Products: -
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol
-
Substrates: the enzyme requires diphosphate in the acceptor substrate
Products: -
Products: -
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol
-
Substrates: the enzyme requires diphosphate in the acceptor substrate
Products: -
Products: -
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol
-
Substrates: the enzyme requires diphosphate in the acceptor substrate
Products: -
Products: -
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol
-
Substrates: the enzyme requires diphosphate in the acceptor substrate
Products: -
Products: -
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-phenoxyundecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-phenoxyundecanol
-
Substrates: the synthetic acceptor substrate D-GlcNAc-alpha-PP-(CH2)11-O-Ph is an amphipathic molecule with similarity to the endogenous substrate D-GlcNAc-alpha-diphospho-ditrans,octacis-undecaprenol. The enzyme requires diphosphate in the acceptor substrate, Strict specificity for UDP-D-galactose as donor substrate
Products: -
Products: -
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-phenoxyundecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-phenoxyundecanol
-
Substrates: the synthetic acceptor substrate D-GlcNAc-alpha-PP-(CH2)11-O-Ph is an amphipathic molecule with similarity to the endogenous substrate D-GlcNAc-alpha-diphospho-ditrans,octacis-undecaprenol. The enzyme requires diphosphate in the acceptor substrate, Strict specificity for UDP-D-galactose as donor substrate
Products: -
Products: -
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-phospho-phenoxyundecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-phospho-phenoxyundecanol
-
Substrates: synthetic acceptor. 11% of the activity with N-acetyl-D-glucosaminyl-alpha-diphospho-phenoxyundecanol
Products: -
Products: -
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-phospho-phenoxyundecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-phospho-phenoxyundecanol
-
Substrates: synthetic acceptor. 11% of the activity with N-acetyl-D-glucosaminyl-alpha-diphospho-phenoxyundecanol
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
Substrates: the O-antigen in the outer membrane of Gram-negative bacteria contains many repeats of an oligosaccharide unit. WfeD catalyzes a step of the repeating-unit assembly in Shigella boydii B14
Products: -
Products: -
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
Substrates: the O-antigen in the outer membrane of Gram-negative bacteria contains many repeats of an oligosaccharide unit. WfeD catalyzes a step of the repeating-unit assembly in Shigella boydii B14
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
5 mM, highest stimulation of activity in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective
Mg2+
-
5 mM, highest stimulation of activityN in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective
Mn2+
-
5 mM, highest stimulation of activity in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective
Ni2+
-
5 mM, highest stimulation of activity in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective
Pb2+
-
5 mM, highest stimulation of activity in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Triton X-100
-
as the concentration of Triton X-100 increases from 0 to 0.5% in the reaction mixture, the activity of the enzyme decreases but is maintained at about 60% of the original activity, up to 6% Triton X-100
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
E101A
-
mutant exhibits minimal enzyme activity, greatly reduced enzyme activity in the presence of either Mn2+ or Pb2+
K211A
-
greatly reduced activity, 60fold higher activity in the presence of Pb2+ than in the presence of Mn2+
E101A
-
mutant exhibits minimal enzyme activity, greatly reduced enzyme activity in the presence of either Mn2+ or Pb2+
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, the crude enzyme preparation retains at least 60% of the initial activity after storage for up to 9 weeks
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Xu, C.; Liu, B.; Hu, B.; Han, Y.; Feng, L.; Allingham, J.S.; Szarek, W.A.
Wang, L.; Brockhausen, I.: Biochemical characterization of UDP-Gal:GlcNAc-pyrophosphate-lipid beta-1,4-Galactosyltransferase WfeD, a new enzyme from Shigella boydii type 14 that catalyzes the second step in O-antigen repeating-unit synthesis
J. Bacteriol.
193
449-459
2010
Shigella boydii, Shigella boydii B14
brenda
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Release 2026.1 (March 2026)
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