Any feedback?
No. of entries
Information on EC 2.4.1.30 - 1,3-beta-oligoglucan phosphorylase
for references in articles please use BRENDA:EC2.4.1.30
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.30 1,3-beta-oligoglucan phosphorylase
IUBMB Comments
Does not act on laminarin. Differs in specificity from EC 2.4.1.31 (laminaribiose phosphorylase) and EC 2.4.1.97 (1,3-β-D-glucan phosphorylase).
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
- 2.4.1.30
- phosphorylases
- gracilis
- synthesis
- gps
- laminaribiose
-
euglena
- oligosaccharide
- glycoside
- polymerization
-
glucosyltransferases
- 1-phosphates
- cellodextrin
-
phosphorolysis
Reaction Schemes
showSynonyms
gh149, pro_7066, laminaridextrin phosphorylase, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
algal beta-1,3-glucan phosphorylase
ANT_17590
AtbetaOGP
beta-1,3-D-glucan phosphorylase
beta-1,3-oligoglucan phosphorylase
beta-1,3-oligoglucan:orthophosphate glucosyltransferase II
-
-
-
-
betaOGP
BOP
laminaridextrin phosphorylase
LOPase
phosphorylase, 1,3-beta-oligoglucan
-
-
-
-
Pro_7066
additional information
beta-1,3-oligoglucan phosphorylase
-
-
-
-
additional information
-
EC 2.4.1.30 and EC 2.4.1.31 catalyze the same reaction but with different quantitative specificity, EC 2.4.1.30 phosphorolyzes laminaritriose and higher homologues at a greater rate than laminaribiose, while the opposite behavior is observed with EC 2.4.1.31 i.e. laminaribiose phosphorylase
additional information
-
EC 2.4.1.30 and EC 2.4.1.31 catalyze the same reaction but with different quantitative specificity, EC 2.4.1.30 phosphorolyzes laminaritriose and higher homologues at a greater rate than laminaribiose, while the opposite behavior is observed with EC 2.4.1.31 i.e. laminaribiose phosphorylase
additional information
-
not identical with laminaribiose phosphorylase, which catalyzes the same reaction
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[(1->3)-beta-D-glucosyl]n + phosphate = [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate = [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
inversion mechanism
-
[(1->3)-beta-D-glucosyl]n + phosphate = [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
(1->3)-beta-D-glucan:phosphate alpha-D-glucosyltransferase
Does not act on laminarin. Differs in specificity from EC 2.4.1.31 (laminaribiose phosphorylase) and EC 2.4.1.97 (1,3-beta-D-glucan phosphorylase).
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
37257-28-6
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-glucose + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
6-deoxy-D-glucose + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
alpha-methylglucoside + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
alpha-phenylglucoside + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosyl-alpha-phenylglucoside + phosphate
-
Substrates: very low activity
Products: -
Products: -
r
arbutin + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosylarbutin + phosphate
-
Substrates: -
Products: -
Products: -
r
beta-D-Glc-(1->3)-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-galactose 1-phosphate
beta-D-Gal-(1->3)-beta-D-Glc-(1->3)-beta-D-Glc-(1->4)-D-Glc + phosphate
-
Substrates: -
Products: -
Products: -
?
beta-D-Glc-(1->4)-beta-D-Glc-(1->3)-D-Glc + alpha-D-galactose 1-phosphate
beta-D-Gal-(1->3)-beta-D-Glc-(1->4)-beta-D-Glc-(1->3)-D-Glc + phosphate
-
Substrates: -
Products: -
Products: -
?
beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-D-Glc + alpha-D-galactose 1-phosphate
beta-D-Gal-(1->3)-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-D-Glc + phosphate
-
Substrates: -
Products: -
Products: -
?
beta-methylglucoside + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosyl-beta-methylglucoside + phosphate
-
Substrates: -
Products: -
Products: -
r
beta-phenylglucoside + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosyl-beta-phenylglucoside + phosphate
-
Substrates: -
Products: -
Products: -
r
cellobiose + alpha-D-galactose 1-phosphate
beta-D-Gal-(1->3)-beta-D-Glc-(1->4)-D-Glc + phosphate
-
Substrates: 0.1% activity compared to alpha-D-glucose 1-phosphate
Products: -
Products: -
?
cellobiose + alpha-D-glucosamine 1-phosphate
beta-D-GlcN-(1->3)-beta-D-Glc-(1->4)-D-Glc + phosphate
-
Substrates: 24% activity compared to alpha-D-glucose 1-phosphate
Products: -
Products: -
?
cellobiose + alpha-D-mannose 1-phosphate
beta-D-Man-(1->3)-beta-D-Glc-(1->4)-D-Glc + phosphate
-
Substrates: 0.4% activity compared to alpha-D-glucose 1-phosphate
Products: -
Products: -
?
fructose + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
gentiobiose + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosylgentiobiose + phosphate
-
Substrates: -
Products: -
Products: -
r
gentiobiose + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
L-arabinose + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
L-idose + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
L-sorbose + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
laminaribiose + alpha-D-galactose 1-phosphate
beta-D-Gal-(1->3)-beta-D-Glc-(1->3)-D-Glc + phosphate
-
Substrates: 0.5% activity compared to alpha-D-glucose 1-phosphate
Products: -
Products: -
?
laminaribiose + alpha-D-glucosamine 1-phosphate
?
-
Substrates: 60% activity compared to alpha-D-glucose 1-phosphate
Products: -
Products: -
?
laminaribiose + alpha-D-mannose 1-phosphate
?
-
Substrates: 2% activity compared to alpha-D-glucose 1-phosphate
Products: -
Products: -
?
laminaribiose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
laminaribiosyl-beta-1,4-glucose + alpha-D-glucose 1-phosphate
laminaritriosyl-beta-1,4-glucose + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaribiosyl-p-hydroquinone + alpha-D-glucose 1-phosphate
laminaritriosyl-p-hydroquinone + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaribiosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritriosyl-saligenin + phosphate
laminaridextrins + alpha-D-glucose 1-phosphate
?
-
Substrates: -
Products: -
Products: -
r
laminariheptaose + alpha-D-glucose 1-phosphate
laminarioctaose + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaripentaose + phosphate
laminaritetraose + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
laminaritetraose + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
laminaritetraosyl-saligenin + alpha-D-glucose 1-phosphate
laminaripentaosyl-saligenin + phosphate
laminaritriose + phosphate
laminaribiose + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
laminaritriosyl-p-hydroquinone + alpha-D-glucose 1-phosphate
laminaritetraosyl-p-hydroquinone + phosphate
laminaritriosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritetraosyl-saligenin + phosphate
phenyl-beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
salicin + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosylsalicin + phosphate
-
Substrates: -
Products: -
Products: -
r
salicin + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
sucrose + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n+1 + phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
Substrates: -
Products: -
Products: -
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
Substrates: role in utilization of the storage polysaccharide paramylon, i.e. beta-1,3-glucan
Products: -
Products: -
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
Substrates: -
Products: -
Products: -
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
Substrates: -
Products: -
Products: -
r
beta-methylglucoside + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
beta-methylglucoside + alpha-D-glucose 1-phosphate
? + phosphate
-
Substrates: -
Products: -
Products: -
r
cellobiose + alpha-D-glucose 1-phosphate
?
-
Substrates: 100% activity
Products: -
Products: -
?
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
Substrates: -
Products: -
Products: -
r
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
-
Substrates: -
Products: -
Products: -
r
cellopentaose + alpha-D-glucose 1-phosphate
cellohexaose + phosphate
Substrates: -
Products: -
Products: -
r
cellopentaose + alpha-D-glucose 1-phosphate
cellohexaose + phosphate
-
Substrates: -
Products: -
Products: -
r
cellotetraose + alpha-D-glucose 1-phosphate
cellopentaose + phosphate
Substrates: -
Products: -
Products: -
r
cellotetraose + alpha-D-glucose 1-phosphate
cellopentaose + phosphate
-
Substrates: -
Products: -
Products: -
r
cellotriose + alpha-D-glucose 1-phosphate
cellotetraose + phosphate
Substrates: -
Products: -
Products: -
r
cellotriose + alpha-D-glucose 1-phosphate
cellotetraose + phosphate
-
Substrates: -
Products: -
Products: -
r
chrysolaminarin + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
chrysolaminarin + alpha-D-glucose 1-phosphate
? + phosphate
-
Substrates: -
Products: -
Products: -
r
D-glucose + alpha-D-glucose 1-phosphate
laminaribiose + phosphate
Substrates: -
Products: -
Products: -
r
D-glucose + alpha-D-glucose 1-phosphate
laminaribiose + phosphate
Substrates: -
Products: -
Products: -
r
D-glucose + alpha-D-glucose 1-phosphate
laminaribiose + phosphate
Anaerolinea thermophila JCM 11388
Substrates: -
Products: -
Products: -
r
D-glucose + alpha-D-glucose 1-phosphate
laminaribiose + phosphate
Anaerolinea thermophila NBRC 100420
Substrates: -
Products: -
Products: -
r
D-glucose + alpha-D-glucose 1-phosphate
laminaribiose + phosphate
Substrates: -
Products: -
Products: -
r
D-glucose + alpha-D-glucose 1-phosphate
laminaribiose + phosphate
-
Substrates: -
Products: -
Products: -
r
D-glucose + alpha-D-glucose 1-phosphate
laminaribiose + phosphate
Substrates: -
Products: -
Products: -
r
D-glucose + alpha-D-glucose 1-phosphate
laminaribiose + phosphate
Substrates: -
Products: -
Products: -
r
D-glucose + alpha-D-glucose 1-phosphate
laminaribiose + phosphate
-
Substrates: -
Products: -
Products: -
r
D-maltose + alpha-D-glucose 1-phosphate
maltotriose + phosphate
Substrates: -
Products: -
Products: -
r
D-maltose + alpha-D-glucose 1-phosphate
maltotriose + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaribiose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaribiose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
-
Substrates: -
Products: product analysis method
Products: product analysis method
r
laminaribiose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
Substrates: -
Products: -
Products: -
r
laminaribiose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
-
Substrates: 100% activity
Products: -
Products: -
?
laminaribiose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaribiosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritriosyl-saligenin + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaribiosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritriosyl-saligenin + phosphate
-
Substrates: best acceptor
Products: -
Products: -
r
laminarihexaose + alpha-D-glucose 1-phosphate
laminariheptaose + phosphate
Substrates: -
Products: -
Products: -
r
laminarihexaose + alpha-D-glucose 1-phosphate
laminariheptaose + phosphate
-
Substrates: -
Products: -
Products: -
r
laminarin + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
laminarin + alpha-D-glucose 1-phosphate
? + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaripentaose + alpha-D-glucose 1-phosphate
laminarihexaose + phosphate
-
Substrates: low activity
Products: -
Products: -
r
laminaripentaose + alpha-D-glucose 1-phosphate
laminarihexaose + phosphate
Substrates: -
Products: -
Products: -
r
laminaripentaose + alpha-D-glucose 1-phosphate
laminarihexaose + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaritetraose + alpha-D-glucose 1-phosphate
laminaripentaose + phosphate
-
Substrates: low activity
Products: -
Products: -
r
laminaritetraose + alpha-D-glucose 1-phosphate
laminaripentaose + phosphate
Substrates: -
Products: -
Products: -
r
laminaritetraose + alpha-D-glucose 1-phosphate
laminaripentaose + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaritetraosyl-saligenin + alpha-D-glucose 1-phosphate
laminaripentaosyl-saligenin + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaritetraosyl-saligenin + alpha-D-glucose 1-phosphate
laminaripentaosyl-saligenin + phosphate
-
Substrates: low activity
Products: -
Products: -
r
laminaritriose + alpha-D-glucose 1-phosphate
laminaritetraose + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaritriose + alpha-D-glucose 1-phosphate
laminaritetraose + phosphate
Substrates: -
Products: -
Products: -
r
laminaritriose + alpha-D-glucose 1-phosphate
laminaritetraose + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaritriosyl-p-hydroquinone + alpha-D-glucose 1-phosphate
laminaritetraosyl-p-hydroquinone + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaritriosyl-p-hydroquinone + alpha-D-glucose 1-phosphate
laminaritetraosyl-p-hydroquinone + phosphate
-
Substrates: low activity
Products: -
Products: -
r
laminaritriosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritetraosyl-saligenin + phosphate
-
Substrates: -
Products: -
Products: -
r
laminaritriosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritetraosyl-saligenin + phosphate
-
Substrates: low activity
Products: -
Products: -
?
sophorose + alpha-D-glucose 1-phosphate
? + phosphate
Substrates: -
Products: -
Products: -
r
sophorose + alpha-D-glucose 1-phosphate
? + phosphate
-
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Anaerolinea thermophila JCM 11388
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Anaerolinea thermophila NBRC 100420
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
?
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
Anaerolinea thermophila JCM 11388
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
Anaerolinea thermophila NBRC 100420
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
-
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
-
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
-
Substrates: -
Products: -
Products: -
r
additional information
?
-
Substrates: analysis of product profile. Specific activity of AtbetaOGP toward alternative acceptor substrates sucrose and fructose in a coupled reaction with sucrose phosphorylase (SP), overview. Bifidobacterium SP (BaSP) and couples its activity to that of AtbetaOGP. BaSP converts sucrose into alphaG1P in the presence of inorganic phosphate (Pi), while AtbetaOGP utilizes the formed glucosyl phosphate to iteratively elongate growing beta-1,3-(oligo)glucan chains, thereby releasing Pi and enabling its recycling
Products: -
Products: -
-
additional information
?
-
Substrates: analysis of product profile. Specific activity of AtbetaOGP toward alternative acceptor substrates sucrose and fructose in a coupled reaction with sucrose phosphorylase (SP), overview. Bifidobacterium SP (BaSP) and couples its activity to that of AtbetaOGP. BaSP converts sucrose into alphaG1P in the presence of inorganic phosphate (Pi), while AtbetaOGP utilizes the formed glucosyl phosphate to iteratively elongate growing beta-1,3-(oligo)glucan chains, thereby releasing Pi and enabling its recycling
Products: -
Products: -
-
additional information
?
-
Anaerolinea thermophila JCM 11388
Substrates: analysis of product profile. Specific activity of AtbetaOGP toward alternative acceptor substrates sucrose and fructose in a coupled reaction with sucrose phosphorylase (SP), overview. Bifidobacterium SP (BaSP) and couples its activity to that of AtbetaOGP. BaSP converts sucrose into alphaG1P in the presence of inorganic phosphate (Pi), while AtbetaOGP utilizes the formed glucosyl phosphate to iteratively elongate growing beta-1,3-(oligo)glucan chains, thereby releasing Pi and enabling its recycling
Products: -
Products: -
-
additional information
?
-
Anaerolinea thermophila NBRC 100420
Substrates: analysis of product profile. Specific activity of AtbetaOGP toward alternative acceptor substrates sucrose and fructose in a coupled reaction with sucrose phosphorylase (SP), overview. Bifidobacterium SP (BaSP) and couples its activity to that of AtbetaOGP. BaSP converts sucrose into alphaG1P in the presence of inorganic phosphate (Pi), while AtbetaOGP utilizes the formed glucosyl phosphate to iteratively elongate growing beta-1,3-(oligo)glucan chains, thereby releasing Pi and enabling its recycling
Products: -
Products: -
-
additional information
?
-
Substrates: analysis of product profile. Specific activity of AtbetaOGP toward alternative acceptor substrates sucrose and fructose in a coupled reaction with sucrose phosphorylase (SP), overview. Bifidobacterium SP (BaSP) and couples its activity to that of AtbetaOGP. BaSP converts sucrose into alphaG1P in the presence of inorganic phosphate (Pi), while AtbetaOGP utilizes the formed glucosyl phosphate to iteratively elongate growing beta-1,3-(oligo)glucan chains, thereby releasing Pi and enabling its recycling
Products: -
Products: -
-
additional information
?
-
-
Substrates: does not act on laminarin and paramylon
Products: -
Products: -
?
additional information
?
-
-
Substrates: EC 2.4.1.30 and EC 2.4.1.31 catalyze the same reaction but with different quantitative specificities, EC 2.4.1.30 phosphorolyzes laminaritriose and higher homologues at a greater rate than laminaribiose, while the opposite behavior is observed with EC 2.4.1.31 i.e. laminaribiose phosphorylase
Products: -
Products: -
?
additional information
?
-
-
Substrates: alpha-methylglucoside and laminarin are no acceptors
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme has a broad acceptor specificity. Substrate are D-glucose, laminaribiose (Lam2), Lam3, Lam4, Lam5, and Lam6, as well as laminarin, allose, mannose, galactose, L-idose, beta-methyl-D-glucoside, 1,5-anhydro-D-glucitol, 2-deoxy-D-glucose, glucosamine, N-acetylglucosamine, D- or L-xylose, sophorose, cellobiose, gentiobiose, phenyl-beta-D-glucoside, O-nitrophenyl-beta-D-glucoside, m-nitrophenyl-beta-D-glucoside, p-nitrophenyl-beta-D-glucoside, salicin, maltose, paramylon treated with KOH, alpha-methylglucoside, beta-methylglucoside, 6-deoxyglucose, lyxose, ribose, L-arabinose, fructose, L-sorbose, sucrose
Products: -
Products: -
-
additional information
?
-
Substrates: crystalline oligosaccharides (DP 30) are produced by a partially purified beta-1,3-D-glucan phosphorylase extract from the microalga Euglena gracilis
Products: -
Products: -
-
additional information
?
-
-
Substrates: the enzyme can synthesize carbohydrates of DP 2-30 with beta-1,3 linkages. Substrates are laminaribiose (Lam2), Lam3, Lam4, Lam5, Lam6, laminarin, cellotriose (Cel3), Cel4, Cel5, sophorose, methyl beta-D-glucopyranoside, and p-nitrophenyl beta-D-glucopyranoside
Products: -
Products: -
-
additional information
?
-
-
Substrates: recombinant enzyme PapP shows neither synthetic activity in the presence of D-Glc and alpha-D-Glc-1P, nor phosphorolysis activity towards Glc-Glc disaccharides with different linkages. Nonetheless, phosphorolysis assays with DP3 to DP6 reveal its preference for longer substrates. These results strongly suggest that PapP can only operate on beta-1,3 linear oligosaccharide acceptors with DP greater than 2, evidencing its different specificity from GH149 glucan phosphorylases
Products: -
Products: -
-
additional information
?
-
-
Substrates: the enzyme can utilize the substrates laminarin, chrysolaminarin, cellobiose, glucose, maltose, and beta-methylglucoside
Products: -
Products: -
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
[(1->3)-beta-D-glucosyl]n + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n+1 + phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
Substrates: -
Products: -
Products: -
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
Substrates: role in utilization of the storage polysaccharide paramylon, i.e. beta-1,3-glucan
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Anaerolinea thermophila JCM 11388
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Anaerolinea thermophila NBRC 100420
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
?
[(1->3)-beta-D-glucosyl]n + phosphate
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
Anaerolinea thermophila JCM 11388
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
Anaerolinea thermophila NBRC 100420
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
Substrates: -
Products: -
Products: -
r
[(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate
-
Substrates: -
Products: -
Products: -
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
Michaelis-Menten kinetics
-
31.6
alpha-D-galactose 1-phosphate
-
with laminatriose as acceptor, at pH 7.0 and 37°C
136.4
alpha-D-galactose 1-phosphate
-
with cellobiose as acceptor, at pH 7.0 and 37°C
0.04
alpha-D-glucosamine 1-phosphate
-
with cellobiose as acceptor, at pH 7.0 and 37°C
0.05
alpha-D-glucosamine 1-phosphate
-
with laminatriose as acceptor, at pH 7.0 and 37°C
2.5
alpha-D-glucose 1-phosphate
-
with cellobiose as acceptor, at pH 7.0 and 37°C
3.1
alpha-D-glucose 1-phosphate
-
with laminatriose as acceptor, at pH 7.0 and 37°C
1.7
alpha-D-mannose 1-phosphate
-
with cellobiose as acceptor, at pH 7.0 and 37°C
2.5
alpha-D-mannose 1-phosphate
-
with laminatriose as acceptor, at pH 7.0 and 37°C
0.56
D-glucose
pH 7.0, 30°C recombinant enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.46
alpha-D-galactose 1-phosphate
-
with laminatriose as acceptor, at pH 7.0 and 37°C
0.77
alpha-D-galactose 1-phosphate
-
with cellobiose as acceptor, at pH 7.0 and 37°C
0.04
alpha-D-glucosamine 1-phosphate
-
with cellobiose as acceptor, at pH 7.0 and 37°C
0.09
alpha-D-glucosamine 1-phosphate
-
with laminatriose as acceptor, at pH 7.0 and 37°C
9.3
alpha-D-glucose 1-phosphate
-
with laminatriose as acceptor, at pH 7.0 and 37°C
10.6
alpha-D-glucose 1-phosphate
-
with cellobiose as acceptor, at pH 7.0 and 37°C
0.03
alpha-D-mannose 1-phosphate
-
with cellobiose as acceptor, at pH 7.0 and 37°C
0.15
alpha-D-mannose 1-phosphate
-
with laminatriose as acceptor, at pH 7.0 and 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.006
alpha-D-galactose 1-phosphate
-
with cellobiose as acceptor, at pH 7.0 and 37°C
0.015
alpha-D-galactose 1-phosphate
-
with laminatriose as acceptor, at pH 7.0 and 37°C
1
alpha-D-glucosamine 1-phosphate
-
with cellobiose as acceptor, at pH 7.0 and 37°C
1.8
alpha-D-glucosamine 1-phosphate
-
with laminatriose as acceptor, at pH 7.0 and 37°C
3
alpha-D-glucose 1-phosphate
-
with laminatriose as acceptor, at pH 7.0 and 37°C
4.2
alpha-D-glucose 1-phosphate
-
with cellobiose as acceptor, at pH 7.0 and 37°C
0.018
alpha-D-mannose 1-phosphate
-
with cellobiose as acceptor, at pH 7.0 and 37°C
0.06
alpha-D-mannose 1-phosphate
-
with laminatriose as acceptor, at pH 7.0 and 37°C
1.99
D-glucose
pH 7.0, 30°C recombinant enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 8.5
activity range, profile overview, over 80% of maximal activity at pH 6.5-7.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45 - 75
activity range, profile overview, inactivation at 80°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
metabolism
physiological function
additional information
evolution
-
enzyme BOP belongs to the glycoside family 94, GH94
evolution
the enzyme belongs to glycoside family 149, GH149
evolution
-
the enzyme belongs to glycoside family 149, GH149. Although the overall domain organisation is similar to GH94, Pro_7066 enzyme contains two additional distinct domains flanking its catalytic region and a surface oligosaccharide binding site where laminarihexaose is accommodated, which is distant from the catalytic site and may be involved in the recognition of longer substrates
metabolism
-
beta-glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of beta-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of beta-glucans as major bioactive compounds. Three different phosphorylase specificities have been described that involve the disaccharide laminaribiose or beta-1,3-glucans, i.e., laminaribiose phosphorylase (LBP, EC 2.4.1.31), beta-1,3-oligoglucan phosphorylase (BOP, EC 2.4.1.30), and beta-1,3-polyglucan or laminarin phosphorylase (BGP, EC 2.4.1.97). Although all can degrade the characteristic beta-1,3-glycosidic linkage, they exhibit a different preference for the chain length of their substrate
metabolism
-
beta-glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of beta-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of beta-glucans as major bioactive compounds. Three different phosphorylase specificities have been described that involve the disaccharide laminaribiose or beta-1,3-glucans, i.e., laminaribiose phosphorylase (LBP, EC 2.4.1.31), beta-1,3-oligoglucan phosphorylase (BOP, EC 2.4.1.30), and beta-1,3-polyglucan or laminarin phosphorylase (BGP, EC 2.4.1.97). Although all can degrade the characteristic beta-1,3-glycosidic linkage, they exhibit a different preference for the chain length of their substrate
metabolism
beta-glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of beta-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of beta-glucans as major bioactive compounds. Three different phosphorylase specificities have been described that involve the disaccharide laminaribiose or beta-1,3-glucans, i.e., laminaribiose phosphorylase (LBP, EC 2.4.1.31), beta-1,3-oligoglucan phosphorylase (BOP, EC 2.4.1.30), and beta-1,3-polyglucan or laminarin phosphorylase (BGP, EC 2.4.1.97). Although all can degrade the characteristic beta-1,3-glycosidic linkage, they exhibit a different preference for the chain length of their substrate
physiological function
thermostable beta-1,3-oligoglucan phosphorylase can convert bulk sugars into functional fibers. The enzyme displays a 20fold higher catalytic efficiency than any betaOGP or betaGP reported to date, and it conveniently synthesizes insoluble beta-1,3-glucans up to DP 75 from glucose as a priming substrate
physiological function
thermostable beta-1,3-oligoglucan phosphorylase can convert bulk sugars into functional fibers. The enzyme displays a 20fold higher catalytic efficiency than any betaOGP or betaGP reported to date, and it conveniently synthesizes insoluble beta-1,3-glucans up to DP 75 from glucose as a priming substrate
physiological function
-
thermostable beta-1,3-oligoglucan phosphorylase can convert bulk sugars into functional fibers. The enzyme displays a 20fold higher catalytic efficiency than any betaOGP or betaGP reported to date, and it conveniently synthesizes insoluble beta-1,3-glucans up to DP 75 from glucose as a priming substrate
-
physiological function
Anaerolinea thermophila JCM 11388
-
thermostable beta-1,3-oligoglucan phosphorylase can convert bulk sugars into functional fibers. The enzyme displays a 20fold higher catalytic efficiency than any betaOGP or betaGP reported to date, and it conveniently synthesizes insoluble beta-1,3-glucans up to DP 75 from glucose as a priming substrate
-
physiological function
Anaerolinea thermophila NBRC 100420
-
thermostable beta-1,3-oligoglucan phosphorylase can convert bulk sugars into functional fibers. The enzyme displays a 20fold higher catalytic efficiency than any betaOGP or betaGP reported to date, and it conveniently synthesizes insoluble beta-1,3-glucans up to DP 75 from glucose as a priming substrate
-
physiological function
-
thermostable beta-1,3-oligoglucan phosphorylase can convert bulk sugars into functional fibers. The enzyme displays a 20fold higher catalytic efficiency than any betaOGP or betaGP reported to date, and it conveniently synthesizes insoluble beta-1,3-glucans up to DP 75 from glucose as a priming substrate
-
additional information
comparisons of the beta-1,3-(oligo)glucan product profiles of betaOGPs from Anaerolinea thermophila (AtbetaOGP) and Euglena gracilis (EgbetaOGP)
additional information
comparisons of the beta-1,3-(oligo)glucan product profiles of betaOGPs from Anaerolinea thermophila (AtbetatOGP) and Euglena gracilis (EgbetaOGP)
additional information
-
comparisons of the beta-1,3-(oligo)glucan product profiles of betaOGPs from Anaerolinea thermophila (AtbetaOGP) and Euglena gracilis (EgbetaOGP)
-
additional information
Anaerolinea thermophila JCM 11388
-
comparisons of the beta-1,3-(oligo)glucan product profiles of betaOGPs from Anaerolinea thermophila (AtbetaOGP) and Euglena gracilis (EgbetaOGP)
-
additional information
Anaerolinea thermophila NBRC 100420
-
comparisons of the beta-1,3-(oligo)glucan product profiles of betaOGPs from Anaerolinea thermophila (AtbetaOGP) and Euglena gracilis (EgbetaOGP)
-
additional information
-
comparisons of the beta-1,3-(oligo)glucan product profiles of betaOGPs from Anaerolinea thermophila (AtbetaOGP) and Euglena gracilis (EgbetaOGP)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A2I6SUQ9_EUGGR
1154
0
127402
TrEMBL
-
A0A8D4WWT7_EUGGR
1150
0
127104
TrEMBL
Secretory Pathway (Reliability: 1)
E8N5S1_ANATU
Anaerolinea thermophila (strain DSM 14523 / JCM 11388 / NBRC 100420 / UNI-1)
1161
0
132282
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme crystal structure in the absence of substrate (PDB ID 6HQ6) and in complex with laminarihexaose (PDB ID 6HQ8)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
coupling of enzye AtbetaOGP with a thermostable variant of Bifidobacterium adolescentis sucrose phosphorylase enables the efficient production of tailorable beta-1,3-(oligo)glucans from sucrose, with a near-complete conversion of over 99 mol%
additional information
-
coupling of enzye AtbetaOGP with a thermostable variant of Bifidobacterium adolescentis sucrose phosphorylase enables the efficient production of tailorable beta-1,3-(oligo)glucans from sucrose, with a near-complete conversion of over 99 mol%
-
additional information
Anaerolinea thermophila JCM 11388
-
coupling of enzye AtbetaOGP with a thermostable variant of Bifidobacterium adolescentis sucrose phosphorylase enables the efficient production of tailorable beta-1,3-(oligo)glucans from sucrose, with a near-complete conversion of over 99 mol%
-
additional information
Anaerolinea thermophila NBRC 100420
-
coupling of enzye AtbetaOGP with a thermostable variant of Bifidobacterium adolescentis sucrose phosphorylase enables the efficient production of tailorable beta-1,3-(oligo)glucans from sucrose, with a near-complete conversion of over 99 mol%
-
additional information
-
coupling of enzye AtbetaOGP with a thermostable variant of Bifidobacterium adolescentis sucrose phosphorylase enables the efficient production of tailorable beta-1,3-(oligo)glucans from sucrose, with a near-complete conversion of over 99 mol%
-
additional information
the inability of some beta-1,3-oligoglucan phosphorylases (BOPs) and beta-1,3-polyglucan (or laminarin) phosphorylases (BGPs, EC 2.4.1.97) to use glucose as an acceptor for beta-glucan synthesis can be overcome through coupled reactions with laminaribiose phosphorylases (EC 2.4.1.31)
additional information
coupling of enzye AtbetaOGP with a thermostable variant of Bifidobacterium adolescentis sucrose phosphorylase enables the efficient production of tailorable beta-1,3-(oligo)glucans from sucrose, with a near-complete conversion of over 99 mol%
additional information
-
the inability of some beta-1,3-oligoglucan phosphorylases (BOPs) and beta-1,3-polyglucan (or laminarin) phosphorylases (BGPs, EC 2.4.1.97) to use glucose as an acceptor for beta-glucan synthesis can be overcome through coupled reactions with laminaribiose phosphorylases (EC 2.4.1.31)
additional information
-
the inability of some beta-1,3-oligoglucan phosphorylases (BOPs) and beta-1,3-polyglucan (or laminarin) phosphorylases (BGPs, EC 2.4.1.97) to use glucose as an acceptor for beta-glucan synthesis can be overcome through coupled reactions with laminaribiose phosphorylases (EC 2.4.1.31)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
without 2-mercaptoethanol: loss of 80% activity after 2 weeks and over 95% after 8 weeks in enzyme extracts, with 2-mercaptoethanol: loss of 50% activity after 8 weeks
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, dialysis, and ultrafiltration
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant expression of His-tagged enzyme in Escherichia coli
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
synthesis
-
synthesis of laminarioligosaccharides, degree of polymerization is dependent on the glucose/glucose 1-phosphate ratio, overview
synthesis
-
synthesis of laminarioligosaccharides by combined action of laminaribiose phosphorylase EC 2.4.1.31 and beta-1,3-oligoglucan phosphorylase EC 2.4.1.30
synthesis
-
beta-1,3-glucans draw considerable attention for their proven beneficial effects on immunomodulation, cholesterol levels, and glycemic control, and their use as additives in food or moisturizing personal care products. Usage of the enzyme for beta-1,3-glucan synthesis
synthesis
-
beta-1,3-glucans draw considerable attention for their proven beneficial effects on immunomodulation, cholesterol levels, and glycemic control, and their use as additives in food or moisturizing personal care products. Usage of the enzyme for beta-1,3-glucan synthesis
synthesis
beta-1,3-glucans draw considerable attention for their proven beneficial effects on immunomodulation, cholesterol levels, and glycemic control, and their use as additives in food or moisturizing personal care products. Usage of the enzyme for beta-1,3-glucan synthesis
synthesis
glycoside phosphorylases are promising catalysts for the bottom-up synthesis of beta-1,3-(oligo)glucans since they combine strict regioselectivity with a cheap donor substrate (i.e., alpha-glucose 1-phosphate). But the need for an expensive priming substrate (e.g., laminaribiose) and the tendency to produce shorter oligosaccharides still major bottlenecks. The thermostable beta-1,3-oligoglucan phosphorylase can be useful in converting bulk sugars into functional fibers. Coupling of enzye AtbetaOGP with a thermostable variant of Bifidobacterium adolescentis sucrose phosphorylase enables the efficient production of tailorable beta-1,3-(oligo)glucans from sucrose, with a near-complete conversion of over 99 mol%
synthesis
glycoside phosphorylases are promising catalysts for the bottom-up synthesis of beta-1,3-(oligo)glucans since they combine strict regioselectivity with a cheap donor substrate (i.e., alpha-glucose 1-phosphate). But the need for an expensive priming substrate (e.g., laminaribiose) and the tendency to produce shorter oligosaccharides still major bottlenecks. The thermostable beta-1,3-oligoglucan phosphorylase can be useful in converting bulk sugars into functional fibers. Coupling of enzyme AtbetaOGP with a thermostable variant of Bifidobacterium adolescentis sucrose phosphorylase enables the efficient production of tailorable beta-1,3-(oligo)glucans from sucrose, with a near-complete conversion of over 99 mol%
synthesis
-
synthesis of laminarioligosaccharides by combined action of laminaribiose phosphorylase EC 2.4.1.31 and beta-1,3-oligoglucan phosphorylase EC 2.4.1.30
-
synthesis
-
glycoside phosphorylases are promising catalysts for the bottom-up synthesis of beta-1,3-(oligo)glucans since they combine strict regioselectivity with a cheap donor substrate (i.e., alpha-glucose 1-phosphate). But the need for an expensive priming substrate (e.g., laminaribiose) and the tendency to produce shorter oligosaccharides still major bottlenecks. The thermostable beta-1,3-oligoglucan phosphorylase can be useful in converting bulk sugars into functional fibers. Coupling of enzye AtbetaOGP with a thermostable variant of Bifidobacterium adolescentis sucrose phosphorylase enables the efficient production of tailorable beta-1,3-(oligo)glucans from sucrose, with a near-complete conversion of over 99 mol%
-
synthesis
Anaerolinea thermophila JCM 11388
-
glycoside phosphorylases are promising catalysts for the bottom-up synthesis of beta-1,3-(oligo)glucans since they combine strict regioselectivity with a cheap donor substrate (i.e., alpha-glucose 1-phosphate). But the need for an expensive priming substrate (e.g., laminaribiose) and the tendency to produce shorter oligosaccharides still major bottlenecks. The thermostable beta-1,3-oligoglucan phosphorylase can be useful in converting bulk sugars into functional fibers. Coupling of enzye AtbetaOGP with a thermostable variant of Bifidobacterium adolescentis sucrose phosphorylase enables the efficient production of tailorable beta-1,3-(oligo)glucans from sucrose, with a near-complete conversion of over 99 mol%
-
synthesis
Anaerolinea thermophila NBRC 100420
-
glycoside phosphorylases are promising catalysts for the bottom-up synthesis of beta-1,3-(oligo)glucans since they combine strict regioselectivity with a cheap donor substrate (i.e., alpha-glucose 1-phosphate). But the need for an expensive priming substrate (e.g., laminaribiose) and the tendency to produce shorter oligosaccharides still major bottlenecks. The thermostable beta-1,3-oligoglucan phosphorylase can be useful in converting bulk sugars into functional fibers. Coupling of enzye AtbetaOGP with a thermostable variant of Bifidobacterium adolescentis sucrose phosphorylase enables the efficient production of tailorable beta-1,3-(oligo)glucans from sucrose, with a near-complete conversion of over 99 mol%
-
synthesis
-
glycoside phosphorylases are promising catalysts for the bottom-up synthesis of beta-1,3-(oligo)glucans since they combine strict regioselectivity with a cheap donor substrate (i.e., alpha-glucose 1-phosphate). But the need for an expensive priming substrate (e.g., laminaribiose) and the tendency to produce shorter oligosaccharides still major bottlenecks. The thermostable beta-1,3-oligoglucan phosphorylase can be useful in converting bulk sugars into functional fibers. Coupling of enzye AtbetaOGP with a thermostable variant of Bifidobacterium adolescentis sucrose phosphorylase enables the efficient production of tailorable beta-1,3-(oligo)glucans from sucrose, with a near-complete conversion of over 99 mol%
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Marechal, L.R.
beta-1,3-Oligoglucan:orthophosphate glucosyltransferases from Euglena gracilis. I. Isolation and some properties of a beta-1,3-oligoglucan phosphorylase
Biochim. Biophys. Acta
146
417-430
1967
Euglena gracilis
brenda
Marechal, L.R.
beta-1,3-Oligoglucan: orthophosphate glucosyltransferases from Euglena gracilis. II. Comparative studies between laminaribiose- and beta-1,3-oligoglucan phosphorylase
Biochim. Biophys. Acta
146
431-442
1967
Euglena gracilis
brenda
Kitaoka, M.; Sasaki, T.; Taniguchi, H.
Synthesis of laminarioligosaccharides using a crude extract of Euglena gracilis z cells
Agric. Biol. Chem.
55
1431-1432
1991
Euglena gracilis, Euglena gracilis Z
-
brenda
Kitaoka, M.; Hayashi, K.
Carbohydrate-processing phosphorolytic enzymes
Trends Glycosci. Glycotechnol.
14
35-50
2002
Euglena gracilis, Poterioochromonas malhamensis
-
brenda
Singh, R.P.; Pergolizzi, G.; Nepogodiev, S.A.; de Andrade, P.; Kuhaudomlarp, S.; Field, R.A.
Preparative and kinetic analysis of beta-1,4- and beta-1,3-glucan phosphorylases informs access to human milk oligosaccharide fragments and analogues thereof
ChemBioChem
21
1043-1049
2020
Homo sapiens
brenda
Ubiparip, Z.; De Doncker, M.; Beerens, K.; Franceus, J.; Desmet, T.
beta-Glucan phosphorylases in carbohydrate synthesis
Appl. Microbiol. Biotechnol.
105
4073-4087
2021
Euglena gracilis (A0A8D4WWT7), Ochromonas danica, Poterioochromonas malhamensis
brenda
Bulmer, G.; de Andrade, P.; Field, R.; van Munster, J.
Recent advances in enzymatic synthesis of beta-glucan and cellulose
Carbohydr. Res.
508
108411
2021
Euglena gracilis (A0A8D4WWT7), Ochromonas sp., Paenibacillus polymyxa, uncultured bacterium
brenda
De Doncker, M.; Vleminckx, S.; Franceus, J.; Vercauteren, R.; Desmet, T.
Converting bulk sugars into functional fibers discovery and application of a thermostable beta-1,3-oligoglucan phosphorylase
J. Agric. Food Chem.
72
10497-10505
2024
Anaerolinea thermophila (E8N5S1), Euglena gracilis var. saccharophila (A0A2I6SUQ9), Anaerolinea thermophila DSM 14523 (E8N5S1), Anaerolinea thermophila JCM 11388 (E8N5S1), Anaerolinea thermophila NBRC 100420 (E8N5S1), Anaerolinea thermophila UNI-1 (E8N5S1)
brenda
EXTERNAL LINKS (specific for EC-Number 2.4.1.30)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
