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Information on EC 2.4.1.289 - N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase and Organism(s) Escherichia coli and UniProt Accession P36667

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IUBMB Comments
Requires Mn2+ or Mg2+. Isolated from Mycobacterium smegmatis and Mycobacterium tuberculosis . The enzyme catalyses the addition of a rhamnosyl unit to N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol, completing the synthesis of the linkage unit that attaches the arabinogalactan moiety to the peptidoglycan moiety in Mycobacterial cell wall.
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This record set is specific for:
Escherichia coli
UNIPROT: P36667
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
lmo1080, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase
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WbbL
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-
-
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PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
dTDP-6-deoxy-beta-L-mannose:N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 3-alpha-L-rhamnosyltransferase
Requires Mn2+ or Mg2+. Isolated from Mycobacterium smegmatis [1] and Mycobacterium tuberculosis [2]. The enzyme catalyses the addition of a rhamnosyl unit to N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol, completing the synthesis of the linkage unit that attaches the arabinogalactan moiety to the peptidoglycan moiety in Mycobacterial cell wall.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene wbbL
UniProt
Manually annotated by BRENDA team
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
the Escherichia coli mutant strain K12 EMG2 contains an insertionally inactivated wbbL and is devoid of any O-antigen and shows no WbbL activity, complementation by expression of WbbL from Mycobacterium tuberculosis, a plasmid encoding the putative rhamnosyltransferase gene from Mycobacterium tuberculosis also results in O-antigen synthesis
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene wbbL, expression in and complementation of Mycobacterium smegmatis strain mc2155 temperature-sensitive mutant 2-20/32
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mills, J.A.; Motichka, K.; Jucker, M.; Wu, H.P.; Uhlik, B.C.; Stern, R.J.; Scherman, M.S.; Vissa, V.D.; Pan, F.; Kundu, M.; Ma, Y.F.; McNeil, M.
Inactivation of the mycobacterial rhamnosyltransferase, which is needed for the formation of the arabinogalactan-peptidoglycan linker, leads to irreversible loss of viability
J. Biol. Chem.
279
43540-43546
2004
Escherichia coli (P36667), Escherichia coli WG1 (P36667), Mycobacterium tuberculosis (P9WMY3), Mycobacterium tuberculosis H37Rv (P9WMY3), Mycolicibacterium smegmatis (Q9RN49), Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 (Q9RN49)
Manually annotated by BRENDA team