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IUBMB Comments The enzyme does not phosphorylate α,α-trehalose, kojibiose, nigerose, or maltose. In the reverse phosphorolysis reaction the enzyme is specific for L -rhamnose as acceptor and β-D -glucose 1-phosphate as donor.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms cphy1019 , more
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cphy1019
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3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate = L-rhamnopyranose + beta-D-glucose 1-phosphate
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3-O-alpha-D-glucopyranosyl-L-rhamnoopyranose:phosphate beta-D-glucosyltransferase
The enzyme does not phosphorylate alpha,alpha-trehalose, kojibiose, nigerose, or maltose. In the reverse phosphorolysis reaction the enzyme is specific for L-rhamnose as acceptor and beta-D-glucose 1-phosphate as donor.
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3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate
L-rhamnose + beta-D-glucose 1-phosphate
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
L-rhamnose + beta-D-glucose 1-phosphate
3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate
additional information
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3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate
L-rhamnose + beta-D-glucose 1-phosphate
Substrates: - Products: -
r
3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate
L-rhamnose + beta-D-glucose 1-phosphate
Substrates: - Products: -
r
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
Substrates: phosphorolysis reaction Products: -
r
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
Substrates: phosphorolysis reaction Products: -
r
L-rhamnose + beta-D-glucose 1-phosphate
3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate
Substrates: - Products: -
r
L-rhamnose + beta-D-glucose 1-phosphate
3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate
Substrates: - Products: -
r
additional information
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Substrates: The enzyme does not phosphorylyze alpha,alpha-trehalose, kojibiose, nigerose, maltose. In the reverse phosphorolysis reaction the enzyme is specific for L-rhamnose as acceptor and beta-D-glucose 1-phosphate as donor. Products: -
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additional information
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Substrates: The enzyme does not phosphorylyze alpha,alpha-trehalose, kojibiose, nigerose, maltose. In the reverse phosphorolysis reaction the enzyme is specific for L-rhamnose as acceptor and beta-D-glucose 1-phosphate as donor. Products: -
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additional information
?
-
Substrates: The enzyme does not phosphorylyze alpha,alpha-trehalose, kojibiose, nigerose, maltose. In the reverse phosphorolysis reaction the enzyme is specific for L-rhamnose as acceptor and beta-D-glucose 1-phosphate as donor. Products: -
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4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
Substrates: phosphorolysis reaction Products: -
r
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
Substrates: phosphorolysis reaction Products: -
r
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9.6
beta-D-glucose 1-phosphate
pH 6.5, 30°C, reverse phosphorolysis reaction
2.7
L-rhamnose
pH 6.5, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
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2.5
beta-D-glucose 1-phosphate
pH 6.5, 30°C, reverse phosphorolysis reaction
1.6
L-rhamnose
pH 6.5, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
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UniProt
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UniProt
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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3OAGR_LACP7
Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
756
0
87991
Swiss-Prot
other Location (Reliability: 3 )
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87990
calculated from amino acid sequence
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expression in Escherichia coli
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Nihira, T.; Nakai, H.; Kitaoka, M.
3-O-alpha-D-glucopyranosyl-L-rhamnose posphorylase from Clostridium phytofermentans
Carbohydr. Res.
350
94-97
2012
Lachnoclostridium phytofermentans (A9KM56), Lachnoclostridium phytofermentans, Lachnoclostridium phytofermentans ATCC 700394 (A9KM56)
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