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Information on EC 2.4.1.280 - N,N'-diacetylchitobiose phosphorylase and Organism(s) Vibrio furnissii and UniProt Accession Q9F8X1

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.280 N,N'-diacetylchitobiose phosphorylase
IUBMB Comments
The enzyme is specific for N,N'-diacetylchitobiose and does not phosphorylate other N-acetylchitooligosaccharides, cellobiose, trehalose, lactose, maltose or sucrose.
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This record set is specific for:
Vibrio furnissii
UNIPROT: Q9F8X1
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Word Map
The taxonomic range for the selected organisms is: Vibrio furnissii
The enzyme appears in selected viruses and cellular organisms
Synonyms
chitobiose phosphorylase, n,n'-diacetylchitobiose phosphorylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer of phosphate
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
N,N'-diacetylchitobiose:phosphate N-acetyl-D-glucosaminyltransferase
The enzyme is specific for N,N'-diacetylchitobiose and does not phosphorylate other N-acetylchitooligosaccharides, cellobiose, trehalose, lactose, maltose or sucrose.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl N,N'-diacetylchitobioside + phosphate
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-D-glucosaminide
show the reaction diagram
reaction at 8.5% the rate of N,N'-diacetylchitobiose
-
-
r
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
show the reaction diagram
-
inversion of the anomeric configuration
-
r
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-beta-D-glucosaminide
4-nitrophenyl N,N'-diacetylchitobioside + phosphate
show the reaction diagram
reverse phosphorolysis, reaction at 36% the rate of N-acetyl-D-glucosamine
-
-
r
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
N,N'-diacetylchitobiose + phosphate
show the reaction diagram
reverse phosphorolysis reaction
-
-
r
additional information
?
-
no substrates are N-acetylchitooligosaccharides, cellobiose, trehalose, lactose, maltose or sucrose
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
show the reaction diagram
-
inversion of the anomeric configuration
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
no requirement for divalent cations
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.58
N,N'-diacetylchitobiose
pH 7.0, 30°C
0.93
N-acetyl-alpha-D-glucosamine 1-phosphate
pH 7.0, 30°C, reverse phosphorolysis reaction
1
N-acetyl-D-glucosamine
pH 7.0, 30°C, reverse phosphorolysis reaction
0.51
phosphate
pH 7.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CHBP_VIBFU
800
0
89667
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
89670
calculated from amino acid sequence
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 37
1 h stable
50
1 h 50% loss of activity
70
1 h compete inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or -80°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Park, J.K.; Keyhani, N.O.; Roseman, S.
Chitin catabolism in the marine bacterium Vibrio furnissii. Identification, molecular cloning, and characterization of A N,N'-diacetylchitobiose phosphorylase
J. Biol. Chem.
275
33077-33083
2000
Vibrio furnissii (Q9F8X1)
Manually annotated by BRENDA team