Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.4.1.280 - N,N'-diacetylchitobiose phosphorylase and Organism(s) Vibrio proteolyticus and UniProt Accession Q76IQ9

for references in articles please use BRENDA:EC2.4.1.280
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.280 N,N'-diacetylchitobiose phosphorylase
IUBMB Comments
The enzyme is specific for N,N'-diacetylchitobiose and does not phosphorylate other N-acetylchitooligosaccharides, cellobiose, trehalose, lactose, maltose or sucrose.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Vibrio proteolyticus
UNIPROT: Q76IQ9
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Vibrio proteolyticus
The enzyme appears in selected viruses and cellular organisms
Synonyms
chitobiose phosphorylase, n,n'-diacetylchitobiose phosphorylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
chitobiose phosphorylase
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer of phosphate
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
N,N'-diacetylchitobiose:phosphate N-acetyl-D-glucosaminyltransferase
The enzyme is specific for N,N'-diacetylchitobiose and does not phosphorylate other N-acetylchitooligosaccharides, cellobiose, trehalose, lactose, maltose or sucrose.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
show the reaction diagram
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
4-methylumbelliferyl N,N'-diacetylchitobioside + phosphate
show the reaction diagram
reverse phosphorolysis, reaction at 20% the rate of N-acetyl-D-glucosamine
-
-
r
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-beta-D-glucosaminide
4-nitrophenyl N,N'-diacetylchitobioside + phosphate
show the reaction diagram
reverse phosphorolysis, reaction at 10% the rate of N-acetyl-D-glucosamine
-
-
r
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
N,N'-diacetylchitobiose + phosphate
show the reaction diagram
reverse phosphorolysis reaction
-
-
r
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
N,N'-diacetylchitobiose
pH 7.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CHBP_VIBPR
801
0
90361
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
150000
gel filtration
90000
2 * 90000, SDS-PAGE
90360
calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 90000, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystals obtained at 4°C using the hanging-drop vapor interactions diffusion method by mixing 5 microl of a protein solution with 5 microl of a reservoir solution comprising 0.1 M HEPES Na-OH (pH 8.0), 0.17 M CaCl2, and 30%-40% (v/v) PEG400
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.1
-
714078
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Honda, Y.; Kitaoka, M.;, Hayashi, K.
Reaction mechanism of chitobiose phosphorylase from Vibrio proteolyticus: identification of family 36 glycosyltransferase in Vibrio
Biochem. J.
377
225-232
2004
Vibrio proteolyticus (Q76IQ9), Vibrio proteolyticus
Manually annotated by BRENDA team
Hidaka, M.; Honda, Y.; Kitaoka, M.; Nirasawa, S.; Hayashi, K.; Wakagi, T.; Shoun, H.; Fushinobu, S.
Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold
Structure
12
937-947
2004
Vibrio proteolyticus (Q76IQ9), Vibrio proteolyticus
Manually annotated by BRENDA team