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IUBMB CommentsThe enzyme is specific for N,N'-diacetylchitobiose and does not phosphorylate other N-acetylchitooligosaccharides, cellobiose, trehalose, lactose, maltose or sucrose.
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N,N'-diacetylchitobiose:phosphate N-acetyl-D-glucosaminyltransferase
The enzyme is specific for N,N'-diacetylchitobiose and does not phosphorylate other N-acetylchitooligosaccharides, cellobiose, trehalose, lactose, maltose or sucrose.
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4-nitrophenyl N,N'-diacetylchitobioside + phosphate
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-D-glucosaminide
reaction at 8.5% the rate of N,N'-diacetylchitobiose
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r
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
4-methylumbelliferyl N,N'-diacetylchitobioside + phosphate
reverse phosphorolysis, reaction at 20% the rate of N-acetyl-D-glucosamine
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r
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-beta-D-glucosaminide
4-nitrophenyl N,N'-diacetylchitobioside + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
N,N'-diacetylchitobiose + phosphate
additional information
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N,N'-diacetylchitobiose + phosphate

N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
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inversion of the anomeric configuration
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r
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
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r
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
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r
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
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inversion of the anomeric configuration
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r
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
the enzymatic phosphorolysis of ChBP begins with the direct nucleophilic attack to the glycosidic bond with the aid of D492, which donates a proton to the glycosidic oxygen atom, and then proceeds through an oxocarbenium cation activated by ion-like transition state
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r
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-beta-D-glucosaminide

4-nitrophenyl N,N'-diacetylchitobioside + phosphate
reverse phosphorolysis, reaction at 36% the rate of N-acetyl-D-glucosamine
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r
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-beta-D-glucosaminide
4-nitrophenyl N,N'-diacetylchitobioside + phosphate
reverse phosphorolysis, reaction at 10% the rate of N-acetyl-D-glucosamine
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r
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate

N,N'-diacetylchitobiose + phosphate
reverse phosphorolysis reaction
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r
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
N,N'-diacetylchitobiose + phosphate
reverse phosphorolysis reaction
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r
additional information

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no substrates are N-acetylchitooligosaccharides, cellobiose, trehalose, lactose, maltose or sucrose
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?
additional information
?
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no substrates are N-acetylchitooligosaccharides
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?
additional information
?
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no substrates are N-acetylchitooligosaccharides
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?
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N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
N,N'-diacetylchitobiose + phosphate

N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
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inversion of the anomeric configuration
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r
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
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-
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r
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
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r
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
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inversion of the anomeric configuration
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r
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
the enzymatic phosphorolysis of ChBP begins with the direct nucleophilic attack to the glycosidic bond with the aid of D492, which donates a proton to the glycosidic oxygen atom, and then proceeds through an oxocarbenium cation activated by ion-like transition state
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r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.