Information on EC 2.4.1.262 - soyasapogenol glucuronosyltransferase

Substrates: enzyme preferentially selects UDP-glucuronic acid as the sugar donor
Products: -

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Ca2+

the effectiveness of divalent cations at 10 mM in activating UGASGT is as follows: Mg2+>Mn2+>Ca2+

Mg2+

the optimum concentration of Mg2+ is between 10 and 25 mM. UGASGT shows an absolute requirement for divalent cations. The effectiveness of divalent cations at 10 mM in activating UGASGT is as follows: Mg2+>Mn2+>Ca2+

Mn2+

the effectiveness of divalent cations at 10 mM in activating UGASGT is as follows: Mg2+>Mn2+>Ca2+

additional information

Na+, K+, Co2+, Fe2+, Zn2+, Cu2+, Al3+, and Fe3+, activate UGASGT only slightly or not at all

show all columns Go to Inhibitor Search

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

Mega-10

the detergent inactivates UGASGT during solubilization

SM-1000

the detergent inactivates UGASGT during solubilization

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.00403

soyasapogenol A

in 100 mM Tris-HCl (pH 8.0), at 40°C

0.01722

soyasapogenol B

in 100 mM Tris-HCl (pH 8.0), at 40°C

0.04194

soyasapogenol E

in 100 mM Tris-HCl (pH 8.0), at 40°C

0.02394

UDP-D-glucuronate

using soyasapogenol B as the sugar acceptor, in 100 mM Tris-HCl (pH 8.0), at 40°C

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.342

crude extract, in 100 mM Tris-HCl (pH 8.0), at 40°C

after 204.68fold purification, in 100 mM Tris-HCl (pH 8.0), at 40°C

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

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pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

5 - 9

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

in Tris-HCl buffer (pH 8.0)

show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

brenda

UniProt

brenda

Lotus japonicus

A0A7I6P8A1

UniProt

brenda

brenda

UniProt

brenda

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

brenda

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

brenda

5783

brenda

brenda

predicted to be a transmembrane protein with two transmembrane helices at the N terminus and three or more at the C terminus

16020

brenda

membrane

predicted to be a transmembrane protein with two transmembrane helices at the N terminus and three or more at the C terminus

brenda

predicted to be a transmembrane protein with two transmembrane helices at the N terminus and three or more at the C terminus

brenda

brenda

brenda

Highest Expressing Human Cell Lines

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Cell Line Links

Gene Links

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

A0A7I6P8A1 pBLAST

A0A7I6P8A1_LOTJA

Lotus japonicus

694

78725

TrEMBL

Show Sequence

other Location (Reliability: 4)

A0A7I6P8N6 pBLAST

A0A7I6P8N6_GLYUR

695

79099

TrEMBL

Show Sequence

I1KFY7 pBLAST

I1KFY7_SOYBN

699

79573

TrEMBL

Show Sequence

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

50000

the 66000 Da and/or the 50000 Da proteins are the UGASGT protein as determined by SDS-PAGE

66000

the 66000 Da and/or the 50000 Da proteins are the UGASGT protein as determined by SDS-PAGE

Go to General Stability Search

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

CHAPS > deoxy-BIGCHAP > n-octyl-beta-D-thioglucoside. Sodium cholate, SM-1200, and n-dodecyl-beta-D-maltoside do not effectively solubilize UGASGT

the effectiveness of detergents at the CMC in solubilizing UGASGT is as follows: MEGA10 > SM-1000 >

Go to Purification (commentary) Search

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

HiTrap Q column chromatography, Superdex 200 gel filtration, and HiTrap Blue column chromatography column chromatography

Go to Expression Search

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EXPRESSION

ORGANISM

UNIPROT

LITERATURE

expression pattern strongly correlates with that of genes involved in soyasaponin biosynthesis

3 entries

UDP-D-glucuronic acid: soyasapogenol B glucuronosyltransferase activity in the methyl jasmonate-treated cells is 2times higher than that in the control cells

Glycyrrhiza glabra

716490

expression pattern strongly correlates with that of genes involved in soyasaponin biosynthesis

I1KFY7

expression pattern strongly correlates with that of genes involved in soyasaponin biosynthesis

expression pattern strongly correlates with that of genes involved in soyasaponin biosynthesis

Lotus japonicus

A0A7I6P8A1

top print hide References Search

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

716490

Hayashi, H.; Huang, P.; Inoue, K.

Up-regulation of soyasaponin biosynthesis by methyl jasmonate in cultured cells of Glycyrrhiza glabra

Plant Cell Physiol.

404-411

2003

Glycyrrhiza glabra

12721381

brenda

Kurosawa, Y.; Takahara, H.; Shiraiwa, M.

UDP-glucuronic acid:soyasapogenol glucuronosyltransferase involved in saponin biosynthesis in germinating soybean seeds

Planta

215

620-629

2002

Glycine max

12172845

brenda