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Information on EC 2.4.1.255 - protein O-GlcNAc transferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q96301
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2.4.1.255 protein O-GlcNAc transferaseIUBMB Comments
Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. EC 2.4.1.255 (protein O-GlcNAc transferase) transfers GlcNAc onto substrate proteins and EC 3.2.1.169 (protein O-GlcNAcase) cleaves GlcNAc from the modified proteins.
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
o-linked n-acetylglucosamine transferase, ogt protein, o-glcnac protein, n-acetylglucosamine transferase, o-glcnac-transferase, human ogt, secret agent, ncogt, o-linked glcnac transferase, o-linked n-acetylglucosaminyltransferase, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
SPY
OGT
SEC
SECRET AGENT
spindly
SPY, protein is similar to animal O-linked N-acetylglucosamine transferase (OGT)
SPY
SPY
SPY shows the same level of similarity with SEC and animal OGTs. SEC and SPY have an overlapping and essential function during embryogenesis
SEC
SEC protein is more similar to animal OGTs than to SPY. SEC and SPY have an overlapping and essential function during embryogenesis
SECRET AGENT
SEC, protein is similar to animal O-linked N-acetylglucosamine transferase (OGT)
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:protein-O-beta-N-acetyl-D-glucosaminyl transferase
Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. EC 2.4.1.255 (protein O-GlcNAc transferase) transfers GlcNAc onto substrate proteins and EC 3.2.1.169 (protein O-GlcNAcase) cleaves GlcNAc from the modified proteins.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
capsid protein of Plum pox virus + UDP-N-acetyl-D-glucosamine
UDP + N-acetyl-D-glucosaminyl-[capsid protein of Plum pox virus]
capsid protein of Plum pox virus + UDP-N-acetyl-D-glucosamine
UDP + N-acetyl-D-glucosaminyl-[capsid protein of Plum pox virus]
capsid protein of Plum pox virus + UDP-N-acetyl-D-glucosamine
UDP + N-acetyl-D-glucosaminyl-[capsid protein of Plum pox virus]
-
-
-
?
capsid protein of Plum pox virus + UDP-N-acetyl-D-glucosamine
UDP + N-acetyl-D-glucosaminyl-[capsid protein of Plum pox virus]
-
-
-
?
capsid protein of Plum pox virus + UDP-N-acetyl-D-glucosamine
UDP + N-acetyl-D-glucosaminyl-[capsid protein of Plum pox virus]
-
-
-
?
capsid protein of Plum pox virus + UDP-N-acetyl-D-glucosamine
UDP + N-acetyl-D-glucosaminyl-[capsid protein of Plum pox virus]
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-
-
?
additional information
?
-
enzyme modifies the Plum pox virus capsid protein
-
-
?
additional information
?
-
enzyme modifies the Plum pox virus capsid protein
-
-
?
additional information
?
-
-
enzyme modifies the Plum pox virus capsid protein
-
-
?
additional information
?
-
participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
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-
?
additional information
?
-
participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
-
-
?
additional information
?
-
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participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
-
-
?
additional information
?
-
catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to Ser/Thr residues of proteins
-
-
?
additional information
?
-
-
catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to Ser/Thr residues of proteins
-
-
?
additional information
?
-
-
enzyme adds a single GlcNAc to hydroxyl groups of serine and threonine residues. In the plant Arabidopsis, OGT serves to attenuate the gibberillin pathway
-
-
?
additional information
?
-
enzyme modifies the Plum pox virus capsid protein
-
-
?
additional information
?
-
enzyme modifies the Plum pox virus capsid protein
-
-
?
additional information
?
-
-
enzyme modifies the Plum pox virus capsid protein
-
-
?
additional information
?
-
participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
-
-
?
additional information
?
-
participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
-
-
?
additional information
?
-
-
participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
enzyme modifies the Plum pox virus capsid protein
-
-
?
additional information
?
-
enzyme modifies the Plum pox virus capsid protein
-
-
?
additional information
?
-
-
enzyme modifies the Plum pox virus capsid protein
-
-
?
additional information
?
-
participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
-
-
?
additional information
?
-
participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
-
-
?
additional information
?
-
-
participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
-
-
?
additional information
?
-
catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to Ser/Thr residues of proteins
-
-
?
additional information
?
-
-
catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to Ser/Thr residues of proteins
-
-
?
additional information
?
-
-
enzyme adds a single GlcNAc to hydroxyl groups of serine and threonine residues. In the plant Arabidopsis, OGT serves to attenuate the gibberillin pathway
-
-
?
additional information
?
-
enzyme modifies the Plum pox virus capsid protein
-
-
?
additional information
?
-
enzyme modifies the Plum pox virus capsid protein
-
-
?
additional information
?
-
-
enzyme modifies the Plum pox virus capsid protein
-
-
?
additional information
?
-
participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
-
-
?
additional information
?
-
participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
-
-
?
additional information
?
-
-
participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
-
-
?
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
loss of function of SECRET AGENT, leads to an early flowering phenotype. This results from reduced histone H3 lysine 4 trimethylation (H3K4me3) of FLOWERING LOCUS C (FLC) locus, which encodes a key negative regulator of flowering
physiological function
the enzyme catalyzes the post-translational modification of proteins. the enzyme (SEC) activates ARABIDOPSIS HOMOLOG OF TRITHORAX1 (ATX1), a histone lysine methyltransferase (HKMT), through O-GlcNAc modification to augment ATX1-mediated H3K4me3 histone modification at FLC locus. SEC transfers an O-GlcNAc group on Ser947 of ATX1, which resides in the SET domain, thereby activating ATX1
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). SPY_ARATH
914
0
101430
Swiss-Prot
other Location (Reliability: 5)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
three-dimensional structure of the protein domains I and II, conserved amino acid sequences
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
three-dimensional structure of the protein domains I and II, conserved amino acid sequences
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
spy-3
Columbia (Col-0) background, has a Gly-to-Ser substitution in the C-terminal region of the protein. The spy plants have defects in a number of processes, including gibberellin and cytokinin responses, flowering, circadian regulation, and light inhibition of hypocotyls elongation
sec spy double mutant
double-mutant embryos aborted at various stages of development and no double-mutant seedlings are obtained. These results indicate that OGT activity is required during gametogenesis and embryogenesis with lethality occurring when parentally derived SEC, SPY, and/or O-GlcNAcylated proteins become limiting
sec-1
has a tDNA insertion within the exon encoding the ninth tetratricopeptide repeat, no obvious phenotype. Two SEC tDNA insertional mutants are identified and analyzed. sec mutant plants do not exhibit obvious phenotypes, sec and spy mutations have a synthetic lethal interaction
sec-2
contains an insertion within an intron adjacent to exons encoding the putative catalytic portion of the protein, no obvious phenotype. Two SEC tDNA insertional mutants are identified and analyzed. sec mutant plants do not exhibit obvious phenotypes, sec and spy mutations have a synthetic lethal interaction
additional information
additional information
plum pox virus is able to infect the Arabidopsis OGT mutants sec-1, sec-2, and spy-3, but at early times of the infection, both rate of virus spread and accumulation are reduced in sec-1 and sec-2 relative to spy-3 and wild-type plants
additional information
plum pox virus is able to infect the Arabidopsis OGT mutants sec-1, sec-2, and spy-3, but at early times of the infection, both rate of virus spread and accumulation are reduced in sec-1 and sec-2 relative to spy-3 and wild-type plants
additional information
-
plum pox virus is able to infect the Arabidopsis OGT mutants sec-1, sec-2, and spy-3, but at early times of the infection, both rate of virus spread and accumulation are reduced in sec-1 and sec-2 relative to spy-3 and wild-type plants
additional information
-
deletional or disrupting mutation of the Spindly SPY locus affects the enzyme and the plant signaling, e.g. increased gibberillin signaling, altered phenotypes
additional information
deletion mapping and site-directed mutagenesis identified three threonine and a serine located near the N-terminus of PPV-CP that are modified by SEC
additional information
deletion mapping and site-directed mutagenesis identified three threonine and a serine located near the N-terminus of PPV-CP that are modified by SEC
additional information
-
deletion mapping and site-directed mutagenesis identified three threonine and a serine located near the N-terminus of PPV-CP that are modified by SEC
additional information
plum pox virus is able to infect the Arabidopsis OGT mutants sec-1, sec-2, and spy-3, but at early times of the infection, both rate of virus spread and accumulation are reduced in sec-1 and sec-2 relative to spy-3 and wild-type plants
additional information
plum pox virus is able to infect the Arabidopsis OGT mutants sec-1, sec-2, and spy-3, but at early times of the infection, both rate of virus spread and accumulation are reduced in sec-1 and sec-2 relative to spy-3 and wild-type plants
additional information
-
plum pox virus is able to infect the Arabidopsis OGT mutants sec-1, sec-2, and spy-3, but at early times of the infection, both rate of virus spread and accumulation are reduced in sec-1 and sec-2 relative to spy-3 and wild-type plants
additional information
sec-1, Wassilewskija background, T-DNA is inserted into the tetratricopeptide repeat domain, no obvious phenotype
additional information
sec-1, Wassilewskija background, T-DNA is inserted into the tetratricopeptide repeat domain, no obvious phenotype
additional information
-
sec-1, Wassilewskija background, T-DNA is inserted into the tetratricopeptide repeat domain, no obvious phenotype
additional information
sec-2, Columbia (Col-0) background. T-DNA is inserted into an intron adjacent to exons coding for the putative catalytic region of the protein, no obvious phenotype
additional information
sec-2, Columbia (Col-0) background. T-DNA is inserted into an intron adjacent to exons coding for the putative catalytic region of the protein, no obvious phenotype
additional information
-
sec-2, Columbia (Col-0) background. T-DNA is inserted into an intron adjacent to exons coding for the putative catalytic region of the protein, no obvious phenotype
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Roos, M.D.; Hanover, J.A.
Structure of O-linked GlcNAc transferase: Mediator of glycan-dependent signaling
Biochem. Biophys. Res. Commun.
271
275-280
2000
Arabidopsis thaliana, Homo sapiens, no activity in Saccharomyces cerevisiae, no activity in Escherichia coli, Rhodobacter sp.
Chen, D.; Juarez, S.; Hartweck, L.; Alamillo, J.M.; Simon-Mateo, C.; Perez, J.J.; Fernandez-Fernandez, M.R.; Olszewski, N.E.; Garcia, J.A.
Identification of secret agent as the O-GlcNAc transferase that participates in Plum pox virus infection
J. Virol.
79
9381-9387
2005
Arabidopsis thaliana (Q96301), Arabidopsis thaliana (Q9M8Y0), Arabidopsis thaliana, Nicotiana benthamiana
Scott, C.L.; Hartweck, L.M.; de Jesus Perez, J.; Chen, D.; Garcia, J.A.; Olszewski, N.E.
SECRET AGENT, an Arabidopsis thaliana O-GlcNAc transferase, modifies the Plum pox virus capsid protein
FEBS Lett.
580
5829-5835
2006
Arabidopsis thaliana (Q96301), Arabidopsis thaliana (Q9M8Y0), Arabidopsis thaliana
Hartweck, L.M.; Scott, C.L.; Olszewski, N.E.
Two O-linked N-acetylglucosamine transferase genes of Arabidopsis thaliana L. Heynh. have overlapping functions necessary for gamete and seed development
Genetics
161
1279-1291
2002
Arabidopsis thaliana (Q9M8Y0), Arabidopsis thaliana
EXTERNAL LINKS (specific for EC-Number 2.4.1.255)
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