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Information on EC 2.4.1.255 - protein O-GlcNAc transferase and Organism(s) Mus musculus and UniProt Accession Q8CGY8

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.255 protein O-GlcNAc transferase
IUBMB Comments
Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. EC 2.4.1.255 (protein O-GlcNAc transferase) transfers GlcNAc onto substrate proteins and EC 3.2.1.169 (protein O-GlcNAcase) cleaves GlcNAc from the modified proteins.
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This record set is specific for:
Mus musculus
UNIPROT: Q8CGY8
Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The taxonomic range for the selected organisms is: Mus musculus
Synonyms
beta-N-acetylglucosaminidase, EGF domain-specific O-GlcNAc transferase, EGF domain-specific O-linked N-acetylglucosamine transferase, EOGT, EOGT1, glycosyltransferase OGT, Gtf1, GtfA, hOGT, HsOGT, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
EGF domain-specific O-GlcNAc transferase
290558
-
EOGT
290558
-
EOGT1
290558
-
ncOGT
244
-
O-GlcNAc transferase
O-GlcNAc-transferase
244
-
O-linked beta-N-acetylglucosamine transferase
244
-
O-linked beta-N-acetylglucosaminyltransferase
244
-
O-linked GlcNAc transferase
290558
-
O-linked N-acetylglucosamine transferase
290558
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:protein-O-beta-N-acetyl-D-glucosaminyl transferase
Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. EC 2.4.1.255 (protein O-GlcNAc transferase) transfers GlcNAc onto substrate proteins and EC 3.2.1.169 (protein O-GlcNAcase) cleaves GlcNAc from the modified proteins.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-GlcNAc + Abi2 protein
UDP + N-acetly-D-glucosaminyl-[Abi2 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Abl2 protein
UDP + N-acetly-D-glucosaminyl-[Ab12 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Ablim2 protein
UDP + N-acetly-D-glucosaminyl-[Ablim2 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Add1 protein
UDP + N-acetly-D-glucosaminyl-[Add1 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Amot protein
UDP + N-acetly-D-glucosaminyl-[Amot protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Arhgap32 protein
UDP + N-acetly-D-glucosaminyl-[Arhgap32 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Atf2 protein
UDP + N-acetly-D-glucosaminyl-[Atf2 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Clasp2 protein
UDP + N-acetly-D-glucosaminyl-[Clasp2 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Clip1 protein
UDP + N-acetly-D-glucosaminyl-[Clip1 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Cnot4 protein
UDP + N-acetly-D-glucosaminyl-[Cnot4 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Cnskr2 protein
UDP + N-acetly-D-glucosaminyl-[Cnskr2 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Corp1b protein
UDP + N-acetly-D-glucosaminyl-[Corp1b protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Crtc1 protein
UDP + N-acetly-D-glucosaminyl-[Crtc1 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Dlgap2 protein
UDP + N-acetly-D-glucosaminyl-[Dlgap2 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Dvl1 protein
UDP + N-acetly-D-glucosaminyl-[Dv11 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Enah protein
UDP + N-acetly-D-glucosaminyl-[Enah protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Foxk2 protein
UDP + N-acetly-D-glucosaminyl-[Foxk2 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Foxp1 protein
UDP + N-acetly-D-glucosaminyl-[Foxp1 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Frs3 protein
UDP + N-acetly-D-glucosaminyl-[Frs3 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Mamld1 protein
UDP + N-acetly-D-glucosaminyl-[Mamld1 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Nav1 protein
UDP + N-acetly-D-glucosaminyl-[Nav1 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Ncoa1 protein
UDP + N-acetly-D-glucosaminyl-[Ncoa1 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Ncoa2 protein
UDP + N-acetly-D-glucosaminyl-[Ncoa2 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Ncoa5 protein
UDP + N-acetly-D-glucosaminyl-[Ncoa5 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Nfia protein
UDP + N-acetly-D-glucosaminyl-[Nfia protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Notch epidermal growth factor 20 repeat
UDP + N-acetyl-D-gluosaminyl-[Notch epidermal growth factor 20 repeat]
show the reaction diagram
-
-
-
?
UDP-GlcNAc + Nr3c1 protein
UDP + N-acetly-D-glucosaminyl-[Nr3c1 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Plec protein
UDP + N-acetly-D-glucosaminyl-[Plec protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Psd3 protein
UDP + N-acetly-D-glucosaminyl-[Psd3 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Rapgef2 protein
UDP + N-acetly-D-glucosaminyl-[Rapgef2 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Rasgrf2 protein
UDP + N-acetly-D-glucosaminyl-[Rasgrf2 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Sirt2 protein
UDP + N-acetly-D-glucosaminyl-[Sirt2 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Ss18l1 protein
UDP + N-acetly-D-glucosaminyl-[Ss1811 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Stat3 protein
UDP + N-acetly-D-glucosaminyl-[Stat3 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Tab1 protein
UDP + N-acetly-D-glucosaminyl-[Tab1 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Tbr1 protein
UDP + N-acetly-D-glucosaminyl-[Tbr1 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Tle4 protein
UDP + N-acetly-D-glucosaminyl-[Tle4 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Tnik protein
UDP + N-acetly-D-glucosaminyl-[Tnik protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + Znf532 protein
UDP + N-acetly-D-glucosaminyl-[Znf532 protein]
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + glutathione S-transferase
glutathione S-transferase-GlcNAc + UDP
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + glutathione S-transferase-CARM1
glutathione S-transferase-CARM1-GlcNAc + UDP
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + glutathione S-transferase-MYPT1
glutathione S-transferase-MYPT1-GlcNAc + UDP
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + O-GlcNAcase
O-GlcNAcase-GlcNAc + UDP
show the reaction diagram
-
-
-
-
?
UDP-GlcNAc + TRAK1 protein
UDP + N-acetyl-D-glucosaminyl-[TRAK1 protein]
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
enzyme catalyzes the addition of O-linked beta-N-acetylglucosamine (O-GlcNAc) onto serine and threonine residues in response to stimuli or stress analogous to phosphorylation by Ser/Thr-kinases
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activates
Mg2+
-
activates
Mn2+
-
activates, highest activity in the presence of 1 mM Mn2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
not inhibited by EDTA
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Insulin
-
tyrosine phosphorylation of OGT increases significantly after a 10 min insulin treatment
-
UDP-N-acetyl-D-glucosamine
-
-
vanadate
-
tyrosine phosphatase inhibitor sodium vanadate, treatment increases OGT activity. When insulin is added to cells preincubated with sodium vanadate, there is a further increase in OGT activity versus inhibitor alone
additional information
-
insulin signaling stimulates tyrosine phosphorylation of OGT, increases its catalytic activity, affects OGT localization, and results in the O-GlcNAc modification of STAT3, a key kinase and transcription factor in 3T3-L1 adipocytes
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
highest expression
Manually annotated by BRENDA team
-
lowest expression
Manually annotated by BRENDA team
contains very little of the ogt transcripts
Manually annotated by BRENDA team
-
neuro-2a murine neuroblastoma cell, ATCC
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
siRNA-mediated knockdown of the enyzme enhances 26S proteasome assembly and activity
metabolism
-
enzyme activation of by UDP-GlcNAc blocks compound C-induced increases in 26S proteasome assembly and activation
physiological function
-
the enzyme plays a major role in AMPK regulation of the proteasome
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
OGT1_MOUSE
1046
0
116952
Swiss-Prot
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expressed in HEK-293T cells
-
modified with both O-GlcNAc and tyrosine phosphorylation when overexpressed in insect cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Whelan, S.A.; Lane, M.D.; Hart, G.W.
Regulation of the O-linked beta-N-acetylglucosamine transferase by insulin signaling
J. Biol. Chem.
283
21411-21417
2008
Mus musculus
Manually annotated by BRENDA team
Hanover, J.A.; Yu, S.; Lubas, W.B.; Shin, S.H.; Ragano-Caracciola, M.; Kochran, J.; Love, D.C.
Mitochondrial and nucleocytoplasmic isoforms of O-linked GlcNAc transferase encoded by a single mammalian gene
Arch. Biochem. Biophys.
409
287-297
2003
Homo sapiens, Mus musculus, Mus musculus (Q8CGY8), Rattus norvegicus
Manually annotated by BRENDA team
Cheung, W.D.; Sakabe, K.; Housley, M.P.; Dias, W.B.; Hart, G.W.
O-linked beta-N-acetylglucosaminyltransferase substrate specificity is regulated by myosin phosphatase targeting and other interacting proteins
J. Biol. Chem.
283
33935-33941
2008
Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Sakaidani, Y.; Ichiyanagi, N.; Saito, C.; Nomura, T.; Ito, M.; Nishio, Y.; Nadano, D.; Matsuda, T.; Furukawa, K.; Okajima, T.
O-linked-N-acetylglucosamine modification of mammalian Notch receptors by an atypical O-GlcNAc transferase Eogt1
Biochem. Biophys. Res. Commun.
419
14-19
2012
Mus musculus, Mus musculus (Q8CGY8)
Manually annotated by BRENDA team
Xu, J.; Wang, S.; Viollet, B.; Zou, M.H.
Regulation of the proteasome by AMPK in endothelial cells: the role of O-GlcNAc transferase (OGT)
PLoS ONE
7
e36717
2012
Mus musculus
Manually annotated by BRENDA team
Alfaro, J.F.; Gong, C.X.; Monroe, M.E.; Aldrich, J.T.; Clauss, T.R.; Purvine, S.O.; Wang, Z.; Camp, D.G.; Shabanowitz, J.; Stanley, P.; Hart, G.W.; Hunt, D.F.; Yang, F.; Smith, R.D.
Tandem mass spectrometry identifies many mouse brain O-GlcNAcylated proteins including EGF domain-specific O-GlcNAc transferase targets
Proc. Natl. Acad. Sci. USA
109
7280-7285
2012
Mus musculus, Mus musculus (Q8CGY8)
Manually annotated by BRENDA team
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