This entry covers the former separate entry for EC 2.4.1.3 (amylomaltase). The plant enzyme has been termed D-enzyme. An enzymic activity of this nature forms part of the mammalian and yeast glycogen debranching system (see EC 3.2.1.33 amylo-alpha-1,6-glucosidase).
This entry covers the former separate entry for EC 2.4.1.3 (amylomaltase). The plant enzyme has been termed D-enzyme. An enzymic activity of this nature forms part of the mammalian and yeast glycogen debranching system (see EC 3.2.1.33 amylo-alpha-1,6-glucosidase).
chain length distribution of the transfer products ranges from DP2 to about DP20, which is the upper size limit of oligosaccharides resolved with the TLC system used, but presumably even larger oligomers are also formed. Glucose is not observed as a product. The transfer products exclusively contain 1,4-glucosidic linkages
chain length distribution of the transfer products ranges from DP2 to about DP20, which is the upper size limit of oligosaccharides resolved with the TLC system used, but presumably even larger oligomers are also formed. Glucose is not observed as a product. The transfer products exclusively contain 1,4-glucosidic linkages
incubation of the enzyme with starch or its constituents, i.e. amylose and amylopectin, leads to the formation of a set of multiples of maltose (i.e. maltose, maltotetraose, maltohexaose etc.)
incubation of the enzyme with starch or its constituents, i.e. amylose and amylopectin, leads to the formation of a set of multiples of maltose (i.e. maltose, maltotetraose, maltohexaose etc.)
incubation of the enzyme with starch or its constituents, i.e. amylose and amylopectin, leads to the formation of a set of multiples of maltose (i.e. maltose, maltotetraose, maltohexaose etc.)
4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-maltotrioside, 4-methylumbelliferyl-alpha-D-glucopyranoside, and 5-bromo-4-chloro-3-indolyl-alpha-D-glucopyranoside are acceptors for GTase
maltose and maltotriose are not disproportionated, glucose does not function as an acceptor sugar in transfer reactions, glucose also never appears as a reaction product
alpha-D-glucose, beta-D-fructose, D-ribose, D-arabinose, D-xylose, isomaltose, D-trehalose, D-cellobiose, lactose, sucrose, raffinose and N-acetyl-D-glucosamine cannot participate as acceptor sugars in glucanosyl transfer from amylose
polysaccharides, such as soluble starch, amylose and amylopectin, and maltooligosaccharides longer than maltose can be effective maltosyl group donors for the enzyme. All maltooligosaccharides are able to act as acceptor molecules in 4-alpha-glucanotransferase-mediated transfer of glucan segments from amylose
modification of granular corn starch with 4-alpha-glucanotransferase from Thermotoga maritima without induction of gelatinization, analysis of the morphology of the modified starches with light and scanning electron microscopy, the granule integrity is mostly maintained after enzyme treatment, although some granules are partially fragmented, amylose and amylopectin levels can be altered, solubility and paste clarity of themodified starches are much higher than those of raw starch, and it shows thermoreversibility between 4 and 75°C, X-ray diffraction and relative crystallinity, overview
modification of granular corn starch with 4-alpha-glucanotransferase from Thermotoga maritima without induction of gelatinization, analysis of the morphology of the modified starches with light and scanning electron microscopy, the granule integrity is mostly maintained after enzyme treatment, although some granules are partially fragmented, amylose and amylopectin levels can be altered, solubility and paste clarity of themodified starches are much higher than those of raw starch, and it shows thermoreversibility between 4 and 75°C, X-ray diffraction and relative crystallinity, overview
the enzyme catalyses the transfer of maltosyl units from alpha-1,4-linked glucans or malto-oligosaccharides to other alpha-1,4-linked glucans, maltooligosaccharides or glucose
the enzyme catalyses the transfer of maltosyl units from alpha-1,4-linked glucans or malto-oligosaccharides to other alpha-1,4-linked glucans, maltooligosaccharides or glucose
the enzyme is highly specialised on the transfer of maltosyl residues from aalpha-1,4-linked glucans or malto-oligosaccharides to other alpha-1,4-linked glucans, malto-oligosaccharides, or glucose
the enzyme is highly specialised on the transfer of maltosyl residues from aalpha-1,4-linked glucans or malto-oligosaccharides to other alpha-1,4-linked glucans, malto-oligosaccharides, or glucose
Gtase, hanging drop method, inhibitor complex,crystals belong to space group I222, unit-cell dimensions a = 92.6 A, b = 180.3 A, c = 199.2 A, free Gtase crystals a = 94.5 A, b = 181.4 A, c = 197.3 A
TmalphaGT can be used to produce granular corn starch, which contains amylose and amylopectin having lower molecular weights and a thermoreversible gelation property
Molecular analysis of a Clostridium butyricum NCIMB 7423 gene encoding 4-alpha-glucanotransferase and characterization of the recombinant enzyme produced in Escherichia coli