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Information on EC 2.4.1.238 - delphinidin 3,5-di-O-glucoside 3'-O-glucosyltransferase and Organism(s) Gentiana triflora and UniProt Accession Q8H0F2

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IUBMB Comments
Isolated from the plant Gentiana triflora (clustered gentian).
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This record set is specific for:
Gentiana triflora
UNIPROT: Q8H0F2
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The taxonomic range for the selected organisms is: Gentiana triflora
The expected taxonomic range for this enzyme is: Gentiana triflora
Synonyms
3'GT, anthocyanin 3'-O-beta-D-glucosyltransferase, UDP-glucose:anthocyanin 3'-O-glucosyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
UDP-glucose:anthocyanin 3'-O-glucosyltransferase
-
anthocyanin 3'-O-beta-D-glucosyltransferase
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-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + delphinidin 3,5-di-O-beta-D-glucoside = UDP + delphinidin 3,3',5-tri-O-beta-D-glucoside
show the reaction diagram
this enzyme specifically glucosylates the 3'-hydroxy group of delphinidin-3-O-glucosyl-5-O-(6-O-caffeoylglucosyl)-3'-O-(6-O-caffeoylglucoside)-type anthocyanins containing glucose groups at the 3 and 5 positions, acts on delphinidin 3,5-di-O-glucoside in gentian, Gentiana triflora
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer of glycosyl group
-
SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:delphinidin-3,5-di-O-beta-D-glucoside 3'-O-glucosyltransferase
Isolated from the plant Gentiana triflora (clustered gentian).
CAS REGISTRY NUMBER
COMMENTARY hide
380231-41-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside)
UDP + delphinidin 3-O,3'-O-di-beta-D-glucosyl-5-O-(6-O-caffeoyl-beta-D-glucoside)
show the reaction diagram
in vitro, 50.9% of the activity with delphinidin 3,5-diglucoside
-
-
?
UDP-glucose + an anthocyanidin
UDP + an anthocyanidin 3'-O-beta-D-glucoside
show the reaction diagram
strict substrate specificity, 3'GT specifically glucosylates the 3'-hydroxy group of delphinidin-type anthocyanins containing glucose groups at the 3 and 5 positions and specifically uses UDP-glucose as the sugar donor, 3’GT strictly requires three hydroxy groups on the B-ring for its substrates
-
-
?
UDP-glucose + delphinidin
UDP + delphinidin 3'-glucoside
show the reaction diagram
poor substrate, 3.3% of the activity with delphinidin 3,5-diglucoside
-
-
?
UDP-glucose + delphinidin 3,5-di-O-beta-D-glucoside
UDP + delphinidin 3,5,7-tri-O-beta-D-glucoside
show the reaction diagram
3'GT glucosylates the 3'-hydroxy group of delphinidin 3,5-diglucoside, 2fold higher activity than with delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside) as substrate
-
-
?
UDP-glucose + delphinidin 3-glucoside
UDP + delphinidin 3,3'-diglucoside
show the reaction diagram
poor substrate, 2.9% of the activity with delphinidin 3,5-diglucoside
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
key enzyme for blue anthocyanin biosynthesis, biosynthesis of gentiodelphin
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Al3+
80% inhibition
Co2+
69% inhibition
UDP
competitive inhibition
Zn2+
26% inhibition
additional information
not inhibited by Mg2+, Ca2+, Mn2+, EDTA or EGTA
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
delphinidin 3,5-diglucoside
pH 8.5, 30°C, recombinant 3'GT
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.76
UDP
pH 8.5, 30°C, recombinant 3'GT
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var. japonica
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
blue, from flowers at a pre-anthesis stage, 3'GT mRNA is abundant in petals at the first three developmental stages, but dramatically decreased in fully opened flowers
Manually annotated by BRENDA team
additional information
3'GT mRNA is barely detectable in leaves
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
recombinant 3'GT expressed in Escherichia coli JM105
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ANGT_GENTR
482
0
54041
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54037
x * 55000, SDS-PAGE, x * 54037, sequence calculation
55000
x * 55000, SDS-PAGE, x * 54037, sequence calculation
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 55000, SDS-PAGE, x * 54037, sequence calculation
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native 3'GT from petals, recombinant 3'GT expressed in Escherichia coli JM105
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in transgenic Petunia hybrida and in Escherichia coli JM105, sequencing
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
3'GT cDNA is a useful molecular tool for modification of flower color using genetic engineering, production of blue anthocyanins
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fukuchi-Mizutani, M.; Okuhara, H.; Fukui, Y.; Nakao, M.; Katsumoto, Y.; Yonekura-Sakakibara, K.; Kusumi, T.; Hase, T.; Tanaka, Y.
Biochemical and molecular characterization of a novel UDP-glucose:anthocyanin 3'-O-glucosyltransferase, a key enzyme for blue anthocyanin biosynthesis, from gentian
Plant Physiol.
132
1652-1663
2003
Gentiana triflora (Q8H0F2)
Manually annotated by BRENDA team