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Information on EC 2.4.1.232 - initiation-specific alpha-1,6-mannosyltransferase and Organism(s) Schizosaccharomyces pombe and UniProt Accession Q9UTR6

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.232 initiation-specific alpha-1,6-mannosyltransferase
IUBMB Comments
Requires Mn2+. In Saccharomyces cerevisiae, this enzyme catalyses an essential step in the outer chain elongation of N-linked oligosaccharides. Man8GlcNAc and Man9GlcNAc are equally good substrates.
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This record set is specific for:
Schizosaccharomyces pombe
UNIPROT: Q9UTR6
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The taxonomic range for the selected organisms is: Schizosaccharomyces pombe
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
och1p, mnn9p, alpha-1,6-mannosyltransferase, alpha-mannosyltransferase, anp1p, spoch1p, hpoch1, yloch1p, tbalg12, vdoch1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-1,6-mannosyltransferase
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GDP-mannose:glycolipid 1,6-alpha-D-mannosyltransferase
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glycolipid 6-alpha-mannosyltransferase
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glycoprotein alpha1-6-mannosyltransferase
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mannosyltransferase, guanosine diphosphomannose-glycoprotein alpha1-6-
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SpOch1p
och1+ gene product, Schizosaccharomyces pombe
alpha-1,6-mannosyltransferase
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GDP-mannose:glycolipid 1,6-alpha-D-mannosyltransferase
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GDP-mannose:oligosaccharide 1,6-alpha-D-mannosyltransferase
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-
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glycolipid 6-alpha-mannosyltransferase
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-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->6)-D-mannosyl-D-mannose linkage
show the reaction diagram
requires Mn2+, in Saccharomyces cerevisiae, this enzyme catalyses an essential step in the outer chain elongation of N-linked oligosaccharides, Man8GlcNAc and Man9GlcNAc are equally good substrates
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycosyl group transfer
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hexosyl group transfer
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hexosyl group transfer
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-
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
GDP-mannose:oligosaccharide 6-alpha-D-mannosyltransferase
Requires Mn2+. In Saccharomyces cerevisiae, this enzyme catalyses an essential step in the outer chain elongation of N-linked oligosaccharides. Man8GlcNAc and Man9GlcNAc are equally good substrates.
CAS REGISTRY NUMBER
COMMENTARY hide
135622-87-6
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346003-17-6
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346003-17-6
Schizosaccharomyces pombe gene och1+
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-mannose + pyridylaminated Man5GlcNAc2
GDP + pyridylaminated Man6GlcNAc2
show the reaction diagram
incorporates alpha-1,6-linked mannose, poor substrate
-
?
GDP-mannose + pyridylaminated Man6GlcNAc2
GDP + pyridylaminated Man7GlcNAc2
show the reaction diagram
incorporates alpha-1,6-linked mannose
-
?
GDP-mannose + pyridylaminated Man7GlcNAc2
GDP + pyridylaminated Man8GlcNAc2
show the reaction diagram
incorporates alpha-1,6-linked mannose
-
?
GDP-mannose + pyridylaminated Man8GlcNAc2
GDP + pyridylaminated Man9GlcNAc2
show the reaction diagram
incorporates alpha-1,6-linked mannose
-
?
GDP-mannose + pyridylaminated Man9GlcNAc2
GDP + pyridylaminated Man10GlcNAc2
show the reaction diagram
incorporates alpha-1,6-linked mannose
-
?
GDP-mannose + pyridylaminated oligosaccharide acceptor
GDP + pyridylaminated alpha-mannosyl-1,6-oligosaccharide
show the reaction diagram
incorporates alpha-1,6-linked mannose, substrate specificity
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?
GDP-mannose + Man9GlcNAc2-PA
GDP + Man10GlcNAc2-PA
show the reaction diagram
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enzyme incorporates alpha-1,6-linked mannose into the substrate
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-
?
GDP-mannose + pyridylaminated oligosaccharide acceptor
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
och1+ gene expression is induced by NaCl and KCl through the transcription factor Atf1p
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strains JY741 and L972
SwissProt
Manually annotated by BRENDA team
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
W266stop
inactive mutant strain 7X4 with a nonsense mutation resulting in a truncated Och1p, temperature- and hygromycin B-sensitive, no synthesis of outer chains on acid phosphatase
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
och1+ gene, sequencing
och1+ gene encodes alpha-1,6-mannosyltransferase, recombinant expression and cloning, och1+ gene knockout fission yeast
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transcriptional control of the och1+ gene, gene expression is not regulated during the cell cycle, but is induced by NaCl and KCl through the transcription factor Atf1p
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yamamoto, K.; Okamoto, M.; Yoko-o, T.; Jigami, Y.
Salt stress induces the expression of the Schizosaccharomyces pombe och1+, which encodes an initiation-specific alpha-1,6-mannosyltransferase for N-linked outer chain synthesis of cell wall mannoproteins
Biosci. Biotechnol. Biochem.
67
927-929
2003
Schizosaccharomyces pombe, Schizosaccharomyces pombe Och1p
Manually annotated by BRENDA team
Tsukahara, K.; Watanabe, T.; Yokoo, T.; Chigami, Y.
Schizosaccharomyces pombe och1+ gene encoding alpha-1,6-mannosyltransferase and use of och1+ gene knockout fission yeast for production of glycoprteins with reduced glycosylation
Jpn. Kokai Tokkyo Koho
11pp
2001
Schizosaccharomyces pombe, Schizosaccharomyces pombe Och1p
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Manually annotated by BRENDA team
Yoko-o, T.; Tsukahara, K.; Watanabe, T.; Hata-Sugi, N.; Yoshimatsu, K.; Nagasu, T.; Jigami, Y.
Schizosaccharomyces pombe och1+ encodes alpha-1,6-mannosyltransferase that is involved in outer chain elongation of N-linked oligosaccharides
FEBS Lett.
489
75-80
2001
Schizosaccharomyces pombe (Q9UTR6), Schizosaccharomyces pombe
Manually annotated by BRENDA team