We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 2.4.1.229 - [Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase for references in articles please use BRENDA:EC2.4.1.229Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The enzyme appears in viruses and cellular organisms
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
[Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-N-acetyl-alpha-D-glucosamine + [Skp1-protein]-trans-4-hydroxy-L-proline = UDP + [Skp1-protein]-O-(N-acetyl-alpha-D-glucosaminyl)-trans-4-hydroxy-L-proline
; Requires dithiothreitol and a divalent cation for activity. This enzyme commences the building up of a pentasaccharide (Galalpha1-6Galalpha1-L-Fucalpha1-2Galbeta1-3GlcNAc) on Hyp-143 of the Dictyostelium protein Skp1, which is required for the ubiquitination of cell-cycle regulatory proteins and transcription factors. The fucose residue is probably in the alpha configuration. The specificity of the enzyme for Skp1-Hyp-143 and its high affinity for this substrate suggests that it is the GlcNAc-transferase that modifies Skp1 in vivo
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hexosyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-N-acetyl-alpha-D-glucosamine:[Skp1-protein]-trans-4-hydroxy-L-proline N-acetyl-alpha-D-glucosaminyl-transferase
Skp1 is a cytoplasmic and nuclear protein required for the ubiquitination of cell cycle regulatory proteins and transcriptional factors. In Dictyostelium Skp1 is modified by the linear pentasaccharide Galalpha1-6Galalpha1-L-Fucalpha1-2Galbeta1-3GlcNAc, which is attached to a hydroxyproline residue at position 143. This enzyme catalyses the first step in the building up of the pentasaccharide by attaching an N-acetylglucosaminyl group to the hydroxyproline residue. It requires dithiothreitol and a divalent cation for activity.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-glycoprotein serine/threonine
-
-
-
-
acetylglucosaminyltransferase, uridine diphosphoacetylglucose-protein Skp1 hydroxyproline
-
-
-
-
hydroxyproline-capping enzyme
Skp1 alpha-GlcNAcT1-like enzyme
Skp1-HyPro GlcNAc-transferase
-
-
-
-
UDP-GlcNAc:hydroxyproline polypeptide GlcNAc-transferase
-
-
-
-
UDP-GlcNAc:Skp1-hydroxyproline GlcNAc-transferase
-
-
-
-
UDP-N-acetylglucosamine (GlcNAc):hydroxyproline polypeptide GlcNAc-transferase
-
-
-
-
GnT1
-
-
GnT51
-
hydroxyproline-capping enzyme
-
-
hydroxyproline-capping enzyme
-
-
Skp1 alpha-GlcNAcT1-like enzyme
-
-
Skp1 alpha-GlcNAcT1-like enzyme
-
-
Skp1 alpha-GlcNAcT1-like enzyme
-
-
Skp1 alpha-GlcNAcT1-like enzyme
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
SwissProt
brenda
-
-
-
brenda
gene GnT51
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
metabolism
-
a hypoxia inducible factor-alpha (HIFR) type prolyl 4-hydroxylase, P4H1, and an alpha-N-acetylglucosaminyltransferase Gnt1, sequentially modify proline 143 of Skp1, a subunit of the Skp1/Cullin/F-box protein, SCF, class of E3 ubiquitin ligases
metabolism
-
a hypoxia inducible factor-alpha (HIFR) type prolyl 4-hydroxylase, P4H1, and an alpha-N-acetylglucosaminyltransferase Gnt1, sequentially modify proline 143 of Skp1, a subunit of the Skp1/Cullin/F-box protein, SCF, class of E3 ubiquitin ligases
additional information
-
Gnt1 from Dictyostelium discoideum contains a stretch of 80 Asn residues and other Asn-rich inserts
additional information
-
Gnt1 from Dictyostelium purureum lacks a stretch of 80 Asn residues and other Asn-rich inserts
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-N-acetylglucosamine + hydroxyproline-peptide
UDP + (N-acetyl-D-glucosaminyl)hydroxyproline peptide
UDP-N-acetylglucosamine + polypeptide-hydroxyamino acid
UDP + polypeptide-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyamino acid
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyproline
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
additional information
?
-
UDP-N-acetylglucosamine + hydroxyproline-peptide
UDP + (N-acetyl-D-glucosaminyl)hydroxyproline peptide
-
synthetic peptide substrate
-
?
UDP-N-acetylglucosamine + hydroxyproline-peptide
UDP + (N-acetyl-D-glucosaminyl)hydroxyproline peptide
-
synthetic peptide substrate
-
?
UDP-N-acetylglucosamine + polypeptide-hydroxyamino acid
UDP + polypeptide-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyamino acid
-
-
-
-
?
UDP-N-acetylglucosamine + polypeptide-hydroxyamino acid
UDP + polypeptide-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyamino acid
-
-
-
-
?
UDP-N-acetylglucosamine + polypeptide-hydroxyamino acid
UDP + polypeptide-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyamino acid
-
-
-
-
?
UDP-N-acetylglucosamine + polypeptide-hydroxyamino acid
UDP + polypeptide-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyamino acid
-
-
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyproline
enzyme is involved in O-glycosylation of Skp1-protein and mucin formation
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyproline
enzyme is highly specific for the Skp1-protein
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
-
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
enzyme attaches the first sugar of pentasaccharide chain
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
enzyme attaches the first sugar of pentasaccharide chain
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
hydroxyproline143
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
hydroxyproline143
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
transfer to the hydroxyproline, at the Thr-Pro143 dipeptide, preceded by a coil and succeeded by an alpha-helix in Skp1
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
-
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
transfer to the hydroxyproline, at the Thr-Pro143 dipeptide, preceded by a coil and succeeded by an alpha-helix in Skp1
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
hydroxyproline143
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
hydroxyproline143
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
high affinity for UDP-GlcNAc
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
recombinant protein substrate, deficient in GlcNAc
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
recombinant protein substrate, deficient in GlcNAc
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
part of complex O-linked glycosylation, which is necessary for targeting of Skp-1-protein to the nucleus
-
?
additional information
?
-
-
substrate specificity of recombinant Gnt1 hydrid enzyme, although synthetic 13-mer and 23-mer hydroxyproline peptides are substrates, their activities are much lower even at 5000fold higher concentration, the 40-mer hydroxyproline peptide is also a poor substrate, overview
-
-
-
additional information
?
-
-
substrate specificity of recombinant Gnt1 hydrid enzyme, although synthetic 13-mer and 23-mer hydroxyproline peptides are substrates than Skyp1A, their activities are much lower even at 5000fold higher concentration, the 40-mer hydroxyproline peptide is also a poor substrate, overview
-
-
-
additional information
?
-
-
no activity with nonhydroxylated Skp-1-protein or -peptide
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-N-acetylglucosamine + polypeptide-hydroxyamino acid
UDP + polypeptide-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyamino acid
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyproline
Q8MZN0
enzyme is involved in O-glycosylation of Skp1-protein and mucin formation
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
UDP-N-acetylglucosamine + polypeptide-hydroxyamino acid
UDP + polypeptide-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyamino acid
-
-
-
-
?
UDP-N-acetylglucosamine + polypeptide-hydroxyamino acid
UDP + polypeptide-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyamino acid
-
-
-
-
?
UDP-N-acetylglucosamine + polypeptide-hydroxyamino acid
UDP + polypeptide-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyamino acid
-
-
-
-
?
UDP-N-acetylglucosamine + polypeptide-hydroxyamino acid
UDP + polypeptide-O-(alpha-N-acetyl-D-glucosaminyl)hydroxyamino acid
-
-
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
-
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
Q8T1C6
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
-
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
-
-
?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
part of complex O-linked glycosylation, which is necessary for targeting of Skp-1-protein to the nucleus
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mg2+
-
activates, best at 10 mM
Mn2+
absolutely dependent on
additional information
-
enzyme prefers low salt conditions, absolutely dependent on divalent cations
additional information
enzyme prefers low salt conditions
additional information
-
divalent cations required
additional information
-
enzyme prefers low salt conditions, absolutely dependent on divalent cations
additional information
-
enzyme prefers low salt conditions, absolutely dependent on divalent cations
additional information
-
enzyme prefers low salt conditions, absolutely dependent on divalent cations
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EDTA
-
complete inhibition at 5 mM
Na+
-
inhibitory at concentrations above 0.05 M
additional information
-
Skp-1-protein isoform containing unhydroxylated proline143 is probably inhibitory
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Brij-35
-
slightly stimulating
dithiothreitol
-
activates, best at 5 mM
DTT
absolutely dependent on
NP-40
-
slightly stimulating
Triton X-100
-
slightly stimulating
Tween 20
-
stimulates, less efficient than Tween 80
Uracil
-
stimulates the purified enzyme at 0.1 mM
additional information
-
requires reducing condition
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.6
hydroxyproline-peptide
-
pH 7.8, 30°C
-
0.00056
Skp-1-protein
-
pH 7.8, 30°C
-
0.00016
UDP-GlcNAc
-
pH 7.8, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00023
-
purified enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
neutral pH-optimum
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
brenda
-
-
brenda
additional information
-
no activity in the particulate fraction
-
brenda
additional information
-
away from membranes
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
29600
-
x * 29600, mutant GnT51(1-282), SDS-PAGE
44000
-
x * 44000, mutant GnT51(1-369+), SDS-PAGE
47500
-
x * 47500, wild-type enzyme and mutants K23R, D102A, H104D, and L276S, SDS-PAGE
51000
-
1 * 51000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 29600, mutant GnT51(1-282), SDS-PAGE; x * 44000, mutant GnT51(1-369+), SDS-PAGE; x * 47500, wild-type enzyme and mutants K23R, D102A, H104D, and L276S, SDS-PAGE
monomer
-
1 * 51000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4
-
partially purified enzyme, unstable
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Tween 20 and Tween 80 stabilize
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
recombinant His-tagged hybrid Gnt1, formed of Dictyostelium discoideum Gnt1/Dictyostelium purpureum Gnt1, from Escherichia coli by nickel affinity chromatography
-
recombinant wild-type enzyme and mutants from Escherichia coli as CBD-intein tagged proteins, to near homogeneity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cloning of a hybrid Gnt1, the coding sequence is generated from the 16 amino acid exon 1 of Dictyostelium discoideum Gnt1 and exon 2 of Dictyostelium purpureum Gnt1, and expression in Escherichia coli as His-tagged protein
gene GnT51, DNA sequence determination and analysis, functional expression of wild-type and mutants as fusion proteins with CBD-intein, in Escherichia coli strain ER2566, autocleavage of tag moiety
-
cloning of a hybrid Gnt1, the coding sequence is generated from the 16 amino acid exon 1 of Dictyostelium discoideum Gnt1 and exon 2 of Dictyostelium purpureum Gnt1, and expression in Escherichia coli as His-tagged protein
-
cloning of a hybrid Gnt1, the coding sequence is generated from the 16 amino acid exon 1 of Dictyostelium discoideum Gnt1 and exon 2 of Dictyostelium purpureum Gnt1, and expression in Escherichia coli as His-tagged protein
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D102A
-
site-directed mutagenesis, no remaining activity
H104D
-
site-directed mutagenesis, no remaining activity
K23R
-
site-directed mutagenesis, unaltered enzyme activity
L276S
-
site-directed mutagenesis, 7% remaining activity compared to the wild-type enzyme
additional information
-
C-terminal deletion mutants GnT51(1-282) and GnT51(1-369+) show no remaining activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GNT1_DICDI
423
49069
Swiss-Prot
A0A084G6T6_9PEZI
527
52393
TrEMBL
M5D7M4_STEMA
371
42082
TrEMBL
A0A074TA52_HAMHA
1560
169571
TrEMBL
A0A084G2U2_9PEZI
280
29973
TrEMBL
S8EQK9_TOXGM
Toxoplasma gondii (strain ATCC 50611 / Me49)
1510
164651
TrEMBL
D9T0D8_MICAI
Micromonospora aurantiaca (strain ATCC 27029 / DSM 43813 / BCRC 12538 / CBS 129.76 / JCM 10878 / NBRC 16125 / NRRL B-16091 / INA 9442)
299
34142
TrEMBL
D7FNT7_ECTSI
691
74405
TrEMBL
A0A086MBY1_TOXGO
1510
164628
TrEMBL
A0A125YWN5_TOXGV
Toxoplasma gondii (strain ATCC 50861 / VEG)
1510
164651
TrEMBL
A0A086LID8_TOXGO
1510
164619
TrEMBL
A0A084GED3_9PEZI
358
37160
TrEMBL
A0A084G8P3_9PEZI
689
70457
TrEMBL
D7G8J8_ECTSI
516
59056
TrEMBL
D7FQK5_ECTSI
440
50080
TrEMBL
A0A139XNB9_TOXGO
1510
164682
TrEMBL
A0A086KY81_TOXGO
1510
164610
TrEMBL
F0VNY2_NEOCL
Neospora caninum (strain Liverpool)
1094
117398
TrEMBL
A0A084GGR3_9PEZI
439
42811
TrEMBL
A0A086JX78_TOXGO
1510
164651
TrEMBL
A0A086R070_TOXGO
1510
164664
TrEMBL
A0A2T6IMW7_TOXGO
1510
164619
TrEMBL
S7UM38_TOXGG
Toxoplasma gondii (strain ATCC 50853 / GT1)
1510
164640
TrEMBL
A0A084GCU8_9PEZI
1044
113845
TrEMBL
A0A084G8F0_9PEZI
562
60483
TrEMBL
A0A086PNR1_TOXGO
1510
164443
TrEMBL
A0A2G8Y0S4_TOXGO
1510
164489
TrEMBL
Q8MZN0_DICDI
380
43967
TrEMBL
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
van der Wel, H.; Morris, H.R.; Panico, M.; Paxton, T.; Dell, A.; Kaplan, L.; West, C.M.
Molecular cloning and expression of a UDP-N-acetylglucosamine (GlcNAc):hydroxyproline polypeptide GlcNAc-transferase that modifies Skp1 in the cytoplasm of Dictyostelium
J. Biol. Chem.
277
46328-46337
2002
Dictyostelium discoideum (Q8T1C6)
brenda
Teng-umnuay, P.; van der Wel, H.; West, C.M.
Identification of a UDP-GlcNAc:Skp1-hydroxyproline GlcNAc-transferase in the cytoplasm of Dictyostelium
J. Biol. Chem.
274
36392-36402
1999
Dictyostelium sp.
brenda
West, C.M.; van der Wel, H.; Gaucher, E.A.
Complex glycosylation of Skp1 in Dictyostelium: implications for the modification of other eukaryotic cytoplasmic and nuclear proteins
Glycobiology
12
17R-27R
2002
Dictyostelium discoideum (Q8T1C6), Dictyostelium sp.
brenda
West, C.M.; van der Wel, H.; Sassi, S.; Gaucher, E.A.
Cytoplasmic glycosylation of protein-hydroxyproline and its relationship to other glycosylation pathways
Biochim. Biophys. Acta
1673
29-44
2004
Chlamydomonas reinhardtii, Dictyostelium discoideum (Q8MZN0), Phytophthora sojae, Thalassiosira pseudonana, Toxoplasma gondii
brenda
van der Wel, H.; Johnson, J.M.; Xu, Y.; Karunaratne, C.V.; Wilson, K.D.; Vohra, Y.; Boons, G.J.; Taylor, C.M.; Bendiak, B.; West, C.M.
Requirements for Skp1 processing by cytosolic prolyl 4(trans)-hydroxylase and alpha-N-acetylglucosaminyltransferase enzymes involved in O2 signaling in dictyostelium
Biochemistry
50
1700-1713
2011
Dictyostelium discoideum, Dictyostelium purpureum
brenda
html completed